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    • 1. 发明申请
    • Sequential Method
    • 顺序方法
    • US20090215196A1
    • 2009-08-27
    • US11572316
    • 2005-07-15
    • Bo EkMats HolmquistKarolina OsterlundMats Inganas
    • Bo EkMats HolmquistKarolina OsterlundMats Inganas
    • G01N33/566
    • G01N33/54306B01L3/5027G01N33/54366
    • A method for determination of an analyte in an original liquid sample by performing an inhibition affinity assay in a microchannel structure of a microfluidic device. The main characteristic feature is the steps of: (i) providing the microfluidic device in a form where the microchannel structure comprises a reaction microcavity which a) an inlet end, and b) contains a solid phase with binding sites (BS) for an analyte or an analyte-related entity, (ii) providing a liquid sample (1) containing the analyte or the analyte-related entity within the microchannel structure at the inlet end of the reaction microcavity, and flowing sample (1) through the reaction microcavity under conditions such that the analyte or the analyte-related entity is captured by BS leaving a portion of BS unoccupied (free BS), (iii) providing a liquid sample (2) which contains an analytically detectable analogue of the analyte or of the analyte-related entity, and flowing sample (2) through said reaction microcavity to capture the analogue by free BS, and (iv) measuring the amount of analyte analogue captured in step (iii) and relating this amount to the amount of analyte in an original sample.
    • 一种通过在微流体装置的微通道结构中进行抑制亲和力测定来测定原始液体样品中的分析物的方法。 主要特征是以下步骤:(i)以微通道结构包含反应微腔的形式提供微流体装置,其中a)入口端和b)包含具有用于分析物的结合位点(BS)的固相 或分析物相关实体,(ii)在反应微腔的入口端处提供在微通道结构内含有分析物或分析物相关实体的液体样品(1),并将样品(1)流过反应微腔下 条件,使得分析物或分析物相关实体被BS捕获,留下一部分BS未占用(自由BS),(iii)提供含有分析物或分析物 - 分析物的可分析检测的类似物的液体样品(2) 相关实体和流动样品(2)通过所述反应微腔以通过游离BS捕获类似物,和(iv)测量步骤(iii)中捕获的分析物类似物的量,并将该量与在 原始样本