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    • 5. 发明申请
    • Biomarker of Renal Impairment
    • 肾损伤生物标志物
    • US20140221507A1
    • 2014-08-07
    • US14131212
    • 2012-07-02
    • Jochen SchwenkPeter NilssonMathias Uhlén
    • Jochen SchwenkPeter NilssonMathias Uhlén
    • G01N33/68
    • G01N33/6893G01N33/493G01N2800/347
    • There is provided a method of determining whether a subject belongs to a first or a second group of subjects, wherein the risk of having or developing of a renal impairment is higher in the first group than in the second group, comprising the steps of: a) measuring an amount of fibulin 1 in a sample from the subject to obtain a sample value; b) comparing the sample value to a reference value; and if the sample value is higher than the reference value, c1) concluding that the subject belongs to the first group; and if the sample value is lower than the reference value, c2) concluding that the subject belongs to the second group, wherein the sample is an optionally modified sample derived from urine or blood, such as an optionally diluted serum or plasma sample. Associated means are also provided.
    • 提供了确定受试者是否属于第一组或第二组受试者的方法,其中在第一组中具有或发展肾损伤的风险高于第二组,其包括以下步骤:a )测量来自受试者的样品中的纤维蛋白1的量以获得样品值; b)将样本值与参考值进行比较; 并且如果样本值高于参考值,则c1)认定该受试者属于第一组; 并且如果样品值低于参考值,则c2)断定受试者属于第二组,其中样品是衍生自尿液或血液的任选修饰的样品,例如任选稀释的血清或血浆样品。 还提供了相关的手段。
    • 7. 发明授权
    • Activating a reversibly inactivated immobilized enzyme by release from an immobilizing moiety
    • 通过从固定部分释放来活化可逆灭活的固定化酶
    • US06610479B1
    • 2003-08-26
    • US08952570
    • 1998-02-13
    • Joakim LundebergMathias Uhlén
    • Joakim LundebergMathias Uhlén
    • C12Q168
    • C12Q1/6848C07K14/315C07K14/70535C07K2319/00C12N9/1252C12Q1/686Y10S435/81C12Q2549/107C12Q2527/127
    • Enzymes or catalytic fragments thereof reversibly inactivated by attachment to an immobilizing moiety which may comprise a magnetic particle are activated by release from the immobilizing moiety. The enzyme or fragment may be in the form of a fusion protein that is attached to the immobilizing moiety via a pair of affinity binding partners such that the enzyme or fragment is reversibly inactivated, and release of the fusion protein from the immobilizing moiety activates the enzyme or fragment. Enzymes include DNA polymerases, DNA ligases, Reverse transcriptases and RNA polymerases. The enzyme or fragment may be thermostable, and the fusion protein can be bound to the immobilizing moiety via a heat-labile linkage. Activation of the reversibly inactivated immobilized enzyme or fragment has particular utility in PCR and analogous nucleic acid amplification techniques. A sample containing a nucleic acid is contacted with the immobilized fusion protein, and release of the fusion protein activates the enzyme or fragment to start a first cycle of an amplification reaction. Amplification reactions that may be carried out include Ligase chain reaction (LCR), Self-sustained Sequence Replication (3SR), Reverse transcriptase PCR (RT-PCR), Q-beta replicase amplification reaction and nucleic acid sequence-based amplification (NASBA). Kits for the amplifications may be prepared containing an appropriate reversibly immobilized enzyme in the form of a fusion protein, and primers and/or probe for performing the amplification.
    • 通过与固定部分连接可逆地失活的酶或其催化片段可以由固定化部分释放而被活化。 酶或片段可以是融合蛋白的形式,其通过一对亲和结合配偶体与固定化部分连接,使得酶或片段可逆地失活,并且融合蛋白从固定化部分的释放激活酶 或片段。 酶包括DNA聚合酶,DNA连接酶,逆转录酶和RNA聚合酶。 酶或片段可以是热稳定的,并且融合蛋白可以通过热不稳定连接键与固定部分结合。 可逆灭活的固定化酶或片段的活化在PCR和类似的核酸扩增技术中具有特别的用途。 将含有核酸的样品与固定化的融合蛋白接触,并且融合蛋白的释放激活酶或片段以开始扩增反应的第一循环。 可能进行的扩增反应包括连接酶链反应(LCR),自持序列复制(3SR),逆转录酶PCR(RT-PCR),Q-β复制酶扩增反应和基于核酸序列的扩增(NASBA)。 可以制备用于扩增的试剂盒,其含有融合蛋白形式的合适的可逆固定的酶,以及用于进行扩增的引物和/或探针。