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    • 5. 发明授权
    • Microbial process for detection of toxic substances
    • 用于检测有毒物质的微生物过程
    • US5672470A
    • 1997-09-30
    • US375818
    • 1995-01-20
    • Wolfgang HengstenbergRoman KolarMarko Scholz
    • Wolfgang HengstenbergRoman KolarMarko Scholz
    • C12Q1/18C12N1/20C12Q1/02C12Q1/48C12R1/19
    • C12Q1/48C12Q1/025C12R1/19
    • A microbial process for detection of toxic substances. The bacterial luminescence test so far used to detect toxic substances is extremely fast in its performance and reacts with very high sensitivity to heavy metals such as mercury and lead. However, that is not the case for other toxicologically relevant heavy metals such as cadmium and chromium. The inhibitory effect of toxic substances on the phosphotransferase system of bacteria, in particular, the E. coli mutant 1219 bgl+, is set forth as proof for the presence of toxic substances in samples. This test method responds with high sensitivity to the presence of the heavy metals cadmium and chromium. The test process constitutes a complement to the bacterial luminescence test and makes it possible to quickly exclude the presence of toxicologically relevant concentrations of cadmium and chromium in such cases where only a slight or no effect at all is indicated on the phosphotransferase system.
    • 用于检测有毒物质的微生物过程。 目前用于检测有毒物质的细菌发光测试性能非常快,对重金属(如汞和铅)具有极高的灵敏度。 然而,其他与毒物相关的重金属如镉和铬不是这种情况。 有毒物质对细菌的磷酸转移酶系统,特别是大肠杆菌突变体1219bgl +的抑制作用被证明是样品中有毒物质的存在的证明。 该测试方法对重金属镉和铬的存在具有高灵敏度的响应。 测试过程构成对细菌发光测试的补充,并且可以在磷酸转移酶系统中仅表现出轻微或没有影响的情况下快速排除毒理学相关浓度的镉和铬的存在。