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1. 发明申请

US20090205085A1 Nucleotide and amino acid sequences from Xenorhabdus bovienii strain Xs85831 and uses thereof 审中-公开
公开(公告)号：US20090205085A1
公开(公告)日：2009-08-13
申请号：US12382649
申请日：2009-03-20
申请人： Barry S. Goldman , Karina Krasomil-Osterfeld , Wei Wu
发明人： Barry S. Goldman , Karina Krasomil-Osterfeld , Wei Wu
IPC分类号： A01H5/00 , C12N15/31 , C07K14/00
CPC分类号： C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to nucleic acid sequences from Xenorhabdus, and, in particular, to genomic DNA sequences, and to nucleotide sequences that encode insecticidal proteins from Xenorhabdus bovienii, strain Xs85831. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's, and fragments of rRNA's. In addition, proteins and fragments thereof and antibodies capable of binding specifically to the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, protein fragments, RNA's, and antibodies, for example, for gene identification and analysis, preparation of constructs, and protecting plants from insect infestations.

2. 发明授权

US07629444B1 Nucleotide and amino acid sequences from Xenorhabdus bovienii strain Xs85831 and uses thereof 有权
标题翻译：来自Xenorhabdus bovienii菌株Xs85831的核苷酸和氨基酸序列及其用途
公开(公告)号：US07629444B1
公开(公告)日：2009-12-08
申请号：US11150804
申请日：2005-06-10
申请人： Barry S. Goldman , Karina Krasomil-Osterfeld , Wei Wu
发明人： Barry S. Goldman , Karina Krasomil-Osterfeld , Wei Wu
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to nucleic acid sequences from Xenorhabdus, and, in particular, to genomic DNA sequences, and to nucleotide sequences that encode insecticidal proteins from Xenorhabdus bovienii, strain Xs85831. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's, and fragments of rRNA's. In addition, proteins and fragments thereof and antibodies capable of binding specifically to the proteins are encompassed by the present invention. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, protein fragments, RNA's, and antibodies, for example, for gene identification and analysis, preparation of constructs, and protecting plants from insect infestations.
摘要翻译：本发明涉及来自Xenorhabdus，特别是基因组DNA序列的核酸序列，以及编码来自Xenorhabdus bovienii，Xs85831的杀虫蛋白的核苷酸序列。本发明包括非编码区中存在的核酸分子以及编码蛋白质，蛋白质片段，tRNA，tRNA片段，rRNA和rRNA片段的核酸分子。此外，本发明包括蛋白质及其片段和能够特异性结合蛋白质的抗体。本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段，RNA和抗体的方法，例如用于基因鉴定和分析，构建体的制备和保护植物免受昆虫侵袭。

3. 发明申请

US20090205084A1 Nucleotide and amino acid sequences from xenorhabdus and uses thereof 审中-公开
标题翻译：来自番茄的核苷酸和氨基酸序列及其用途
公开(公告)号：US20090205084A1
公开(公告)日：2009-08-13
申请号：US12382648
申请日：2009-03-20
申请人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
发明人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
IPC分类号： A01H5/00 , C12N15/31 , C07K14/195
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/24 , Y02A40/162
摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.
摘要翻译：本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白和包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

4. 发明授权

US07696340B2 Nucleotide and amino acid sequences from Xenorhabdus and uses thereof 有权
标题翻译：来自Xenorhabdus的核苷酸和氨基酸序列及其用途
公开(公告)号：US07696340B2
公开(公告)日：2010-04-13
申请号：US11998426
申请日：2007-11-30
申请人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
发明人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
IPC分类号： C07H21/04 , C07K17/00 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/24 , Y02A40/162
摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.
摘要翻译：本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白以及包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

5. 发明授权

US07319142B1 Nucleotide and amino acid sequences from Xenorhabdus and uses thereof 有权
标题翻译：来自Xenorhabdus的核苷酸和氨基酸序列及其用途
公开(公告)号：US07319142B1
公开(公告)日：2008-01-15
申请号：US11216782
申请日：2005-08-31
申请人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
发明人： Barry S. Goldman , Karina Krasomil-Osterfeld , Thomas M. Malvar , John W. Pitkin , Steven C. Slater , Wei Wu , Jiamin Zeng
IPC分类号： C07H21/04 , C07K14/00
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/24 , Y02A40/162
摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.
摘要翻译：本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白以及包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

6. 发明申请

US20080090999A1 Nucleotide and amino acid sequences from xenorhabdus and uses thereof 有权
标题翻译：来自番茄的核苷酸和氨基酸序列及其用途
公开(公告)号：US20080090999A1
公开(公告)日：2008-04-17
申请号：US11998426
申请日：2007-11-30
申请人： Barry Goldman , Karina Krasomil-Osterfeld , Thomas Malvat , John Pitkin , Steven Slater , Wei Wu , Jiamin Zeng
发明人： Barry Goldman , Karina Krasomil-Osterfeld , Thomas Malvat , John Pitkin , Steven Slater , Wei Wu , Jiamin Zeng
IPC分类号： C07H21/02
CPC分类号： C12N15/8286 , A01N63/00 , A01N63/02 , C07K14/24 , Y02A40/162
摘要： The invention provides isolated nucleotide sequences from Xenorhabdus nematophila species Xs86068, and, in particular, nucleotide sequences that encode insect inhibitory proteins, the insecticidal proteins, and compositions that comprise one or more of the insecticidal proteins for use in controlling insect infestation.
摘要翻译：本发明提供了来自杀线虫（Xenorhabdus nematophila）Xs86068的分离的核苷酸序列，特别是编码昆虫抑制蛋白的核苷酸序列，杀虫蛋白和包含一种或多种用于防治昆虫侵袭的杀虫蛋白的组合物。

7. 发明授权

US08796026B2 Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor 有权
标题翻译：从苏云金芽孢杆菌分泌的杀虫蛋白并用于其
公开(公告)号：US08796026B2
公开(公告)日：2014-08-05
申请号：US10563270
申请日：2004-07-06
申请人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
发明人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
IPC分类号： C12N15/82 , C12N15/32
CPC分类号： A01N63/02 , C07K14/32
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译：本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

8. 发明申请

US20060191034A1 Insecticidal proteins secreted from bacillus thuringiensis and uses therefor 有权
标题翻译：从苏云金芽孢杆菌分泌的杀虫蛋白，用于此
公开(公告)号：US20060191034A1
公开(公告)日：2006-08-24
申请号：US10563270
申请日：2004-07-06
申请人： James Baum , Judith Donovan , William Donovan , James Engleman , Karina Krasomil-Osterfeld , John Pitkin , James Roberts
发明人： James Baum , Judith Donovan , William Donovan , James Engleman , Karina Krasomil-Osterfeld , John Pitkin , James Roberts
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C07K14/325 , A01N63/00
CPC分类号： A01N63/02 , C07K14/32
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译：本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

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