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1. 发明授权

US08796026B2 Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor 有权
标题翻译：从苏云金芽孢杆菌分泌的杀虫蛋白并用于其
公开(公告)号：US08796026B2
公开(公告)日：2014-08-05
申请号：US10563270
申请日：2004-07-06
申请人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
发明人： James A. Baum , Judith C. Donovan , William P. Donovan , James T. Engleman , Karina Krasomil-Osterfeld , John W. Pitkin , James K. Roberts
IPC分类号： C12N15/82 , C12N15/32
CPC分类号： A01N63/02 , C07K14/32
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译：本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

2. 发明申请

US20110061128A1 IN PLANTA RNAi CONTROL OF FUNGI 审中-公开
标题翻译：真菌的PLANTA RNAi控制
公开(公告)号：US20110061128A1
公开(公告)日：2011-03-10
申请号：US12850544
申请日：2010-08-04
申请人： James K. Roberts , John W. Pitkin , Thomas H. Adams
发明人： James K. Roberts , John W. Pitkin , Thomas H. Adams
IPC分类号： C12N15/82 , C07H21/00 , C07H21/02 , C12N1/00 , C12N5/10 , A01H5/00 , A01H5/10 , A01N57/16 , C07K1/00 , C11B1/00 , C12Q1/68 , A01P3/00 , A23L1/00 , A23K1/00
CPC分类号： C12N15/8282
摘要： The present invention relates to control of fungal and oomycete plant pathogens by inhibiting one or more biological functions. The invention provides methods and compositions for such control. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pathogen, a reduction in disease may be obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic agents for use in protecting plants from pathogen infection.
摘要翻译：本发明涉及通过抑制一种或多种生物学功能来控制真菌和卵菌纲植物病原体。本发明提供了用于这种控制的方法和组合物。通过将本发明提供的一种或多种重组双链RNA分子送入病原体，可以通过抑制基因表达来获得疾病的减少。本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受病原体感染的转基因试剂的特定组合的方法。

3. 发明申请

US20080201801A1 Invertebrate MicroRNAs 有权
标题翻译：无脊椎动物微RNA
公开(公告)号：US20080201801A1
公开(公告)日：2008-08-21
申请号：US12033178
申请日：2008-02-19
申请人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
发明人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
IPC分类号： C12N15/11 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.
摘要翻译：本发明提供具有抗无脊椎害虫的植物。更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

4. 发明授权

US08410334B2 Invertebrate microRNAs 有权
标题翻译：无脊椎动物微RNA
公开(公告)号：US08410334B2
公开(公告)日：2013-04-02
申请号：US12033178
申请日：2008-02-19
申请人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
发明人： Edwards Allen , William P. Donovan , Gregory R. Heck , James K. Roberts , Virginia Ursin , Yuanji Zhang
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/113 , C12N15/8285 , C12N2310/14 , C12N2310/141 , C12N2310/531 , Y02A40/162 , Y02A40/164
摘要： This invention provides a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest, recombinant DNA constructs for expression of at least one invertebrate miRNA in planta and methods of use thereof, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention.
摘要翻译：本发明提供了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫有关的共生体的靶基因，用于将至少一种无脊椎动物miRNA表达的重组DNA构建体植物及其使用方法，含有本发明的非天然转基因植物细胞的非天然转基因植物，从本发明的非天然转基因植物细胞生长的非天然转基因植物和非天然转基因种子由非天然转基因植物产生，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。

5. 发明授权

US08946510B2 Compositions and methods for control of insect infestations in plants 有权
公开(公告)号：US08946510B2
公开(公告)日：2015-02-03
申请号：US11547764
申请日：2005-04-08
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC分类号： C12N15/82 , C12N15/87 , A01H1/00 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

6. 发明授权

US09121035B2 Insecticidal compositions and methods for making insect-resistant transgenic plants 有权
标题翻译：杀虫组合物和制备抗虫转基因植物的方法
公开(公告)号：US09121035B2
公开(公告)日：2015-09-01
申请号：US12064840
申请日：2006-08-30
申请人： James A. Baum , James K. Roberts , Bei Zhang , Heather Anderson , Catherine A. Chay
发明人： James A. Baum , James K. Roberts , Bei Zhang , Heather Anderson , Catherine A. Chay
IPC分类号： C12N15/82 , C07K14/325 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N15/8216 , C12N15/8257 , Y02A40/162
摘要： The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such as TIC 127, in plants. Methods of making and using the polynucleotide sequences and the proteins in the development of transgenic plant cells and transgenic plants exhibiting improved insect resistance against (1) Coleopteran insects including Western Corn Rootworm (Dibrotica virgifera), Southern Corn Rootworm (Dibrotica undecempunctata), Northern Corn Rootworm (Diabrotica barbed), Mexican Corn Rootworm (Diabrotica virgifera zeae), Brazilian Corn Rootworm (Diabrotica balteata) and Brazilian Corn Rootworm complex (Diabrotica viridula and Diabrotica speciosa), and against Hemipteran insects such as Lygus bugs, are disclosed.
摘要翻译：本发明提供编码来自苏云金芽孢杆菌的ET37，TIC810和TIC812蛋白质的分离的多核苷酸序列，以及用于表达TIC809，ET37，TIC810和TIC812的核苷酸序列，以及这些蛋白质如TIC 127在植物中的各种杀虫有效组合的融合。在转基因植物细胞和转基因植物的开发中制备和使用多核苷酸序列和蛋白质的方法和转基因植物对（1）鞘翅目昆虫包括西方玉米根虫（Dibrotica virgifera），南方玉米根虫（Dibrotica undecempunctata），北方玉米披露了根虫（Diabrotica barbed），墨西哥玉米根虫（Diabrotica virgifera zeae），巴西玉米根虫（Diabrotica balteata）和巴西玉米Rootworm综合体（Diabrotica viridula和Diabrotica speciosa），以及针对半翅目昆虫如Lygus虫。

7. 发明申请

US20090307803A1 Compositions and methods for control of insect infestations in plants 有权
标题翻译：控制植物昆虫侵袭的组成和方法
公开(公告)号：US20090307803A1
公开(公告)日：2009-12-10
申请号：US11547764
申请日：2005-04-08
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R.I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC分类号： A01H5/10 , A01N57/00 , A01N33/00 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
摘要翻译：本发明涉及通过抑制无脊椎害虫中的一种或多种生物学功能来控制害虫侵袭。本发明公开了通过将一种或多种不同的重组双链RNA分子加入害虫来控制害虫侵染的方法和组合物，以通过抑制基因表达来实现害虫侵染的减少。本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。

8. 发明授权

US09238822B2 Compositions and methods for control of insect infestations in plants 有权
公开(公告)号：US09238822B2
公开(公告)日：2016-01-19
申请号：US13226353
申请日：2011-09-06
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC分类号： C12N15/82 , C12N15/87 , A01H1/00 , A01N63/02
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

9. 发明申请

US20120164205A1 COMPOSITIONS AND METHODS FOR CONTROL OF INSECT INFESTATIONS IN PLANTS 有权
标题翻译：用于控制植物感染的组合物和方法
公开(公告)号：US20120164205A1
公开(公告)日：2012-06-28
申请号：US13226353
申请日：2011-09-06
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC分类号： A01N25/08 , C12N1/21 , C12N1/15 , A01P7/04 , A01N63/00 , A01N63/04 , A01N37/18 , A01N57/16 , C12N15/113 , C12N1/19
CPC分类号： C12N15/8286 , A01N63/02 , C12N15/8275 , C12N15/8279 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
摘要翻译：本发明涉及通过抑制无脊椎害虫中的一种或多种生物学功能来控制害虫侵袭。本发明公开了通过将一种或多种不同的重组双链RNA分子加入害虫来控制害虫侵染的方法和组合物，以通过抑制基因表达来实现害虫侵染的减少。本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。

10. 发明授权

US07812219B2 Compositions and methods for control of insect infestations in plants 有权
标题翻译：控制植物昆虫侵袭的组成和方法
公开(公告)号：US07812219B2
公开(公告)日：2010-10-12
申请号：US11948759
申请日：2007-11-30
申请人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
发明人： James A. Baum , Larry A. Gilbertson , David K. Kovalic , Thomas J. LaRosa , Maolong Lu , Tichafa R. I. Munyikwa , James K. Roberts , Wei Wu , Bei Zhang
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
摘要翻译：本发明涉及通过抑制无脊椎害虫中的一种或多种生物学功能来控制害虫侵袭。本发明公开了通过将一种或多种不同的重组双链RNA分子加入害虫来控制害虫侵染的方法和组合物，以通过抑制基因表达来实现害虫侵染的减少。本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。

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