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1. 发明授权

US07270013B2 Dispenser volume determination method 有权
标题翻译：分配器体积确定方法
公开(公告)号：US07270013B2
公开(公告)日：2007-09-18
申请号：US11095298
申请日：2005-03-31
申请人： Balwant S. Bhullar , Daniel T. Bourne
发明人： Balwant S. Bhullar , Daniel T. Bourne
IPC分类号： G01F25/00 , G01F13/00 , G01F22/00 , G01N21/84 , G01N21/93
CPC分类号： G01F1/661 , G01F22/00 , G01F25/0092
摘要： The present invention includes methods of determining the volume of liquid dispensed by a dispenser. Initially, a refractive index analysis is preformed on a first liquid that has been dispensed from the dispenser in question. From the refractive index analysis, information is obtained about the volume of first liquid dispensed. The invention also includes methods for verifying the volumetric accuracy of a dispenser. The methods include dispensing a first liquid having a known refractive index into a vessel having a second liquid with a known volume and a known refractive index to form a test mixture. The refractive index of the test mixture is measured and then correlated with the refractive index of the first liquid to obtain information about the volume of first liquid dispensed. The dispensed volume is then compared to the theoretical volume of liquid dispensed by the dispenser.
摘要翻译：本发明包括确定由分配器分配的液体的体积的方法。首先，在从所述分配器分配的第一液体上进行折射率分析。从折射率分析得到关于第一液体分配的体积的信息。本发明还包括用于验证分配器的体积精度的方法。所述方法包括将具有已知折射率的第一液体分配到具有已知体积和已知折射率的第二液体的容器中以形成测试混合物。测量测试混合物的折射率，然后与第一液体的折射率相关，以获得关于分配的第一液体的体积的信息。然后将分配的体积与由分配器分配的液体的理论体积进行比较。

2. 发明授权

US5731157A Two-site allergen immunoassay 失效
标题翻译：两站式过敏原免疫测定
公开(公告)号：US5731157A
公开(公告)日：1998-03-24
申请号：US175715
申请日：1993-12-30
申请人： Larry S. Miller , Balwant S. Bhullar , Richard S. Tuttle , Victor S. Moore
发明人： Larry S. Miller , Balwant S. Bhullar , Richard S. Tuttle , Victor S. Moore
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , Y10S435/963 , Y10S436/808 , Y10S436/826
摘要： An allergen immunoassay method features the use of a combination of a) closely controlled 1) elevated temperatures for assay reactions, 2) low temperatures for reagents and samples, 3) times for assay steps and especially assay reaction times, 4) reagent concentrations, and 5) reagent amounts; b) the use of a fast and accurate method of sample preparation that removes dust and contaminants; c) the stabilization of samples to avoid auto- and antibody degradation and unwanted effects of sample contaminants; and d) the formation of a colored product to determine the amount of a specific allergen. This combination provides an assay that can be completed in a few hours while retaining the precision, accuracy, sensitivity and response curve of previous methods requiring much longer periods of time. The assay is especially suitable for computer control using a robotic liquid distribution system and allows for the determination of four different specific allergens in one hundred sixty samples in duplicate with standards and controls in an eight hour period with a significant reduction in the number of steps and attended technician time over previous assays.
摘要翻译：变应原免疫测定方法的特征在于使用a）严格控制的1）升高的温度进行测定反应，2）试剂和样品的低温，3）测定步骤的时间，特别是测定反应时间，4）试剂浓度，和 5）试剂量; b）使用快速准确的样品制备方法去除灰尘和污染物; c）样品的稳定性，以避免自身和抗体降解以及样品污染物的不良影响; 和d）形成有色产品以确定特定变应原的量。这种组合提供了可以在几个小时内完成的测定，同时保持以前方法需要更长时间的精度，精度，灵敏度和响应曲线。该测定特别适用于使用机器人液体分配系统的计算机控制，并允许在八小时内测定一百六十个样品中的四种不同的特异性过敏原与标准品和对照物一式两份，显着降低步骤数量，在以前的测定中，技术人员的时间比较长。

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