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1. 发明授权

US5731157A Two-site allergen immunoassay 失效
标题翻译：两站式过敏原免疫测定
公开(公告)号：US5731157A
公开(公告)日：1998-03-24
申请号：US175715
申请日：1993-12-30
申请人： Larry S. Miller , Balwant S. Bhullar , Richard S. Tuttle , Victor S. Moore
发明人： Larry S. Miller , Balwant S. Bhullar , Richard S. Tuttle , Victor S. Moore
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , Y10S435/963 , Y10S436/808 , Y10S436/826
摘要： An allergen immunoassay method features the use of a combination of a) closely controlled 1) elevated temperatures for assay reactions, 2) low temperatures for reagents and samples, 3) times for assay steps and especially assay reaction times, 4) reagent concentrations, and 5) reagent amounts; b) the use of a fast and accurate method of sample preparation that removes dust and contaminants; c) the stabilization of samples to avoid auto- and antibody degradation and unwanted effects of sample contaminants; and d) the formation of a colored product to determine the amount of a specific allergen. This combination provides an assay that can be completed in a few hours while retaining the precision, accuracy, sensitivity and response curve of previous methods requiring much longer periods of time. The assay is especially suitable for computer control using a robotic liquid distribution system and allows for the determination of four different specific allergens in one hundred sixty samples in duplicate with standards and controls in an eight hour period with a significant reduction in the number of steps and attended technician time over previous assays.
摘要翻译：变应原免疫测定方法的特征在于使用a）严格控制的1）升高的温度进行测定反应，2）试剂和样品的低温，3）测定步骤的时间，特别是测定反应时间，4）试剂浓度，和 5）试剂量; b）使用快速准确的样品制备方法去除灰尘和污染物; c）样品的稳定性，以避免自身和抗体降解以及样品污染物的不良影响; 和d）形成有色产品以确定特定变应原的量。这种组合提供了可以在几个小时内完成的测定，同时保持以前方法需要更长时间的精度，精度，灵敏度和响应曲线。该测定特别适用于使用机器人液体分配系统的计算机控制，并允许在八小时内测定一百六十个样品中的四种不同的特异性过敏原与标准品和对照物一式两份，显着降低步骤数量，在以前的测定中，技术人员的时间比较长。

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