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1. 发明授权

US10287542B2 Apparatus for automatically preparing cell-free proteins and method for preparing proteins using same 有权
公开(公告)号：US10287542B2
公开(公告)日：2019-05-14
申请号：US14648891
申请日：2013-11-29
申请人： BIONEER CORPORATION
发明人： Han Oh Park , Jong Kab Kim , Ji Won Han , You Sang Cho , Min Jung Kim , Ha Neul Kim , Yang Won Lee , Nam Il Kim
IPC分类号： C07K1/14 , C12M1/00 , C12M1/24 , C12M1/32 , C12M1/26 , C12M1/34 , C12P21/00 , C07K1/02 , C07K1/04 , C07K1/22 , C07K1/36
摘要： An automated cell-free protein production system comprises: a protein expression reaction unit comprising a reaction vessel that includes a plurality of dialysis tubes, each including a dialysis membrane and being open at its top; a reaction temperature control unit configured to heat or cool the reaction vessel; a pipette array comprising a plurality of pipettes and configured to suck or discharge solutions using the pipettes; a pipette array moving unit configured to move the pipette array in an upward and downward direction, a forward and backward direction or a left and right direction so as to move solutions; a protein purification unit including a magnetic field application device; and a multi-well plate mounting unit having mounted therein a multi-well plate kit configured to supply solutions that are used for protein production.

2. 发明授权

US09765383B2 Apparatus and method for automatically analyzing biological samples 有权
公开(公告)号：US09765383B2
公开(公告)日：2017-09-19
申请号：US14377537
申请日：2013-02-07
申请人： BIONEER CORPORATION
发明人： Han Oh Park , Yang Won Lee , Jong-Kab Kim
IPC分类号： C12M1/00 , C12Q1/68 , G01N35/00 , B01L7/00 , G01N35/10
CPC分类号： C12Q1/6804 , B01L7/52 , G01N35/0099 , G01N35/1065
摘要： Provided are an apparatus and a method for automatically analyzing biological samples capable of performing the entire processes of dissolving the biological samples in protease and cell lysate to dissolve a nucleic acid in a solution, attaching the nucleic acid to magnetic particles, finally washing the magnetic particle to which the nucleic acid is attached with an organic solvent, drying the magnetic particles using a vacuum pump, eluting the target nucleic acid attached to the magnetic particles in an aqueous solution, adding and mixing the eluted target nucleic acid into a vessel containing a nucleic acid amplification reagent, real-time detecting amplification by irradiating excitation light to a reactor simultaneously with regulating a temperature to perform amplification to measure fluorescence, inactivating an amplified product using an ultraviolet lamp after amplification, obtaining an image through electrophoresis, and analyzing a molecular weight in a single apparatus.

3. 发明申请

US20150037803A1 APPARATUS AND METHOD FOR AUTOMATICALLY ANALYZING BIOLOGICAL SAMPLES 有权
标题翻译：用于自动分析生物样品的装置和方法
公开(公告)号：US20150037803A1
公开(公告)日：2015-02-05
申请号：US14377537
申请日：2013-02-07
申请人： BIONEER CORPORATION
发明人： Han Oh Park , Yang Won Lee , Jong-Kab Kim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , B01L7/52 , G01N35/0099 , G01N35/1065
摘要： Provided are an apparatus and a method for automatically analyzing biological samples capable of performing the entire processes of dissolving the biological samples in protease and cell lysate to dissolve a nucleic acid in a solution, attaching the nucleic acid to magnetic particles, finally washing the magnetic particle to which the nucleic acid is attached with an organic solvent, drying the magnetic particles using a vacuum pump, eluting the target nucleic acid attached to the magnetic particles in an aqueous solution, adding and mixing the eluted target nucleic acid into a vessel containing a nucleic acid amplification reagent, real-time detecting amplification by irradiating excitation light to a reactor simultaneously with regulating a temperature to perform amplification to measure fluorescence, inactivating an amplified product using an ultraviolet lamp after amplification, obtaining an image through electrophoresis, and analyzing a molecular weight in a single apparatus.
摘要翻译：提供了一种用于自动分析生物样品的装置和方法，其能够执行将生物样品溶解在蛋白酶和细胞裂解物中的整个过程，以将核酸溶解在溶液中，将核酸附着到磁性颗粒上，最后洗涤磁性颗粒核酸与有机溶剂连接在其上，使用真空泵干燥磁性颗粒，用水溶液洗脱附着在磁性颗粒上的靶核酸，加入并将洗脱的目标核酸混合到含有核酸的容器中酸扩增试剂，通过在调节温度的同时照射激发光同时进行扩增以测量荧光，扩增后的紫外线灯灭活扩增产物，通过电泳获得图像，分析分子量，实时检测扩增在一个单一的设备。

4. 发明申请

US20220176373A1 POLYMERASE CHAIN REACTION SYSTEM 有权
公开(公告)号：US20220176373A1
公开(公告)日：2022-06-09
申请号：US17602665
申请日：2020-04-09
申请人： Bioneer Corporation
发明人： Han Oh Park , Jong Kab Kim , Yang Won Lee , Sang Ryoung Park , Hye Jin Jang
IPC分类号： B01L3/00 , B01L7/00 , C12Q1/6806
摘要： The present invention relates to a system structure capable of performing real-time detection of nucleic acid extraction and amplification reactions and amplified results in a device for implementing a polymerase chain reaction (PCR). A PCR system includes a nucleic acid extraction cartridge configured to extract a nucleic acid of a biological sample via a nucleic acid extraction reagent stored therein and form a PCR preliminary mixture by being additionally mixed with a polymerase reaction dried product, a PCR plate inserted into the nucleic acid extraction cartridge, having a channel coupled to the nucleic acid extraction cartridge, and receives a nucleic acid solution or the PCR preliminary mixture extracted from the nucleic acid extraction cartridge and diversely accommodates a PCR reaction dried product containing a dried primer/probe or a primer/probe in at least one reaction well, a temperature control module disposed above the PCR plate 200 and including a pair of heating blocks 310 and 320 adjacent to the reaction well (W) to apply different temperatures, and a scanning module scanning a concentration of a reactant amplified in the reaction well (W).

5. 发明授权

US11175300B2 Biological sample processing apparatus 有权
公开(公告)号：US11175300B2
公开(公告)日：2021-11-16
申请号：US16338218
申请日：2017-09-28
申请人： BIONEER CORPORATION
发明人： Han Oh Park , Yang Won Lee , Jin Il Lee , Jung Do Kim , Dae Jin Jang , Jong Kab Kim
IPC分类号： G01N35/04 , G01N35/10 , G01N35/00 , G01N35/02 , B01L3/02
摘要： A biological sample processing apparatus, including: a pipette block with which a plurality of pipettes for sucking or discharging a biological sample in a multi-well plate in which wells are arranged in a matrix shape along row and column directions are detachably coupled; a pipette block forward and backward transfer unit configured to move the pipette block along a forward and backward direction along a process direction; a pipette block top and bottom transfer unit configured to move the pipette block along a vertical direction; a magnetic field applying unit disposed below the multi-well plate for applying a magnetic field to a well of the multi-well plate; and a heating unit disposed below the multi-well plate so as to be spaced apart from the magnetic field applying unit, for heating a well of the multi-well plate.

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