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1. 发明授权

US07767419B2 Calibrator for NT-proBNP immunoassay 有权
标题翻译：用于NT-proBNP免疫测定的校准物
公开(公告)号：US07767419B2
公开(公告)日：2010-08-03
申请号：US10359028
申请日：2003-02-04
申请人： Azhar Alvi , William Yajima , Roshana Sikora , George Jackowski , Mee-Ra Hong
发明人： Azhar Alvi , William Yajima , Roshana Sikora , George Jackowski , Mee-Ra Hong
IPC分类号： C12N15/12 , C12N15/63 , C12N15/70 , C12P19/34 , G01N33/531 , C07H21/04
CPC分类号： G01N33/74 , G01N2333/58 , Y10S436/811 , Y10S530/806 , Y10T436/10
摘要： The invention provides a method to efficiently express high levels of a recombinant untagged NT-proBNP for use as a calibrator in NT-proBNP immunoassays.
摘要翻译：本发明提供了一种在NT-proBNP免疫测定中有效表达高水平重组未标记的NT-proBNP用作校准物的方法。

2. 发明授权

US08293972B2 Recombinant antibodies to Sclerotinia antigens 有权
标题翻译：核盘菌抗原重组抗体
公开(公告)号：US08293972B2
公开(公告)日：2012-10-23
申请号：US12916078
申请日：2010-10-29
申请人： Nataraj Kav , William Yajima , Bo Yang
发明人： Nataraj Kav , William Yajima , Bo Yang
IPC分类号： A01H5/00 , A01H5/10 , C12N15/09 , C12N15/82
CPC分类号： G01N33/56961 , C07K16/14 , C07K2317/622 , C07K2317/76 , C12N15/8209 , C12N15/8258 , C12N15/8282
摘要： The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.
摘要翻译：本发明涉及与核盘菌核菌抗原结合并包含单链可变片段（scFv）的重组抗体。抗原可以选自SSPG1d或其一部分，天冬氨酰蛋白酶或其部分，或整个核盘菌核菌菌丝体。本发明还提供了与抗真菌多肽连接的抗体。本发明延伸到编码抗体的核酸序列和包含核酸序列的表达载体。本发明还涉及用表达载体转化的转基因植物，种子，组织或细胞。还提供了利用结合核盘菌属抗原的抗体的生物样品中产生对核盘菌的抗性并用于检测核盘菌的核酸的转基因植物的方法和用于其的免疫测定试剂盒。

3. 发明授权

US07910702B2 Recombinant antibodies to sclerotinia antigens 有权
标题翻译：核盘菌抗原的重组抗体
公开(公告)号：US07910702B2
公开(公告)日：2011-03-22
申请号：US11829513
申请日：2007-07-27
申请人： Nataraj Kav , William Yajima , Bo Yang
发明人： Nataraj Kav , William Yajima , Bo Yang
IPC分类号： C12P21/08 , G01N33/02
CPC分类号： G01N33/56961 , C07K16/14 , C07K2317/622 , C07K2317/76 , C12N15/8209 , C12N15/8258 , C12N15/8282
摘要： The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.
摘要翻译：本发明涉及与核盘菌核菌抗原结合并包含单链可变片段（scFv）的重组抗体。抗原可以选自SSPG1d或其一部分，天冬氨酰蛋白酶或其部分，或整个核盘菌核菌菌丝体。本发明还提供了与抗真菌多肽连接的抗体。本发明延伸到编码抗体的核酸序列和包含核酸序列的表达载体。本发明还涉及用表达载体转化的转基因植物，种子，组织或细胞。还提供了利用结合核盘菌属抗原的抗体的生物样品中产生对核盘菌的抗性并用于检测核盘菌的核酸的转基因植物的方法和用于其的免疫测定试剂盒。

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