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1. 发明申请

US20120040870A1 Method For Assembly Of Polynucleic Acid Sequences 有权
公开(公告)号：US20120040870A1
公开(公告)日：2012-02-16
申请号：US13140473
申请日：2009-12-18
申请人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty
发明人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty
IPC分类号： C12P19/34 , C40B50/06
CPC分类号： C12N15/66 , C12N15/1093 , C12N15/64
摘要： The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13 at the 3′-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25′ at the 5′-end of the nucleic acid sequence, wherein the 5′-end linker sequence L25′ of nucleic acid sequence N2 is complementary to the 3′-end linker sequence L13′ of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3′-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3′-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5′-end, wherein for the first additional nucleic acid sequence N3 the 5′-end linker sequence L35′ is complementary to the 3′-end linker sequence L23′ of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5′-end linker sequence is complementary to the 3′-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence.

2. 发明授权

US09777305B2 Method for the assembly of a polynucleic acid sequence 有权
公开(公告)号：US09777305B2
公开(公告)日：2017-10-03
申请号：US13806358
申请日：2011-06-23
申请人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty , Maiwenn Kersaudy Kerhoas , Farid Amalou , Will Shu
发明人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty , Maiwenn Kersaudy Kerhoas , Farid Amalou , Will Shu
IPC分类号： C12P19/34 , B01F11/00 , B01F11/02 , B01F13/00 , B01F13/08 , C12N15/10 , C12Q1/68 , C12N15/66 , B01L3/00 , B01L7/00
CPC分类号： C12P19/34 , B01F11/0051 , B01F11/0071 , B01F11/0241 , B01F13/0059 , B01F13/0818 , B01F2215/0454 , B01L3/502707 , B01L3/502715 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/025 , B01L2200/04 , B01L2300/044 , B01L2300/0867 , B01L2300/0887 , B01L2400/043 , B01L2400/0655 , C12N15/10 , C12N15/1031 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q2565/629 , C12Q2525/191 , C12Q2521/501
摘要： Provided herein are methods for the assembly of a polynucleic acid sequence that is at least partially carried out on a microfluidic device; methods for the preparation of a library of polynucleic acid sequences; microfluidic devices; methods for designing nucleic acid sequences; methods for planning the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences; systems comprising components for carrying out these methods; computer programs which, when run on a computer, implements these methods; and computer readable medium or carrier signals encoding such a computer program.

3. 发明授权

US08999679B2 Method for assembly of polynucleic acid sequences 有权
公开(公告)号：US08999679B2
公开(公告)日：2015-04-07
申请号：US13140473
申请日：2009-12-18
申请人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty
发明人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty
IPC分类号： C12N15/66 , C12N15/10 , C12N15/64
CPC分类号： C12N15/66 , C12N15/1093 , C12N15/64
摘要： The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13 at the 3′-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25′ at the 5′-end of the nucleic acid sequence, wherein the 5′-end linker sequence L25′ of nucleic acid sequence N2 is complementary to the 3′-end linker sequence L13′ of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3′-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3′-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5′-end, wherein for the first additional nucleic acid sequence N3 the 5′-end linker sequence L35′ is complementary to the 3′-end linker sequence L23′ of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5′-end linker sequence is complementary to the 3′-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence.

4. 发明申请

US20140030766A1 Method And Device 有权
公开(公告)号：US20140030766A1
公开(公告)日：2014-01-30
申请号：US13806358
申请日：2011-06-23
申请人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty , Maiwenn Kersaudy Kerhoas , Farid Amalou , Wenmiao Shu
发明人： Austin Che , Tom Knight , Barry Canton , Jason Kelly , Reshma Shetty , Maiwenn Kersaudy Kerhoas , Farid Amalou , Wenmiao Shu
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , B01F11/0051 , B01F11/0071 , B01F11/0241 , B01F13/0059 , B01F13/0818 , B01F2215/0454 , B01L3/502707 , B01L3/502715 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/025 , B01L2200/04 , B01L2300/044 , B01L2300/0867 , B01L2300/0887 , B01L2400/043 , B01L2400/0655 , C12N15/10 , C12N15/1031 , C12N15/1093 , C12N15/66 , C12Q1/6806 , C12Q2565/629 , C12Q2525/191 , C12Q2521/501
摘要： The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13′ at the 3′-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25′ at the 5′-end of the nucleic acid sequence, wherein the 5′-end linker sequence L25′ of nucleic acid sequence N2 is complementary to the 3′-end linker sequence L13′ of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L23′ at the 3′-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3′-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3′-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5′-end, wherein for the first additional nucleic acid sequence N3 the 5′-end linker sequence L35′ is complementary to the 3′-end linker sequence L23′ of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5′-end linker sequence is complementary to the 3′-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence; wherein at least step (iv) is carried out on a microfluidic device.

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