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    • 5. 发明授权
    • Array for rapid detection of a microorganism
    • 用于快速检测微生物的阵列
    • US08617874B2
    • 2013-12-31
    • US12464149
    • 2009-05-12
    • Stephanie Michelle MartinJohn Gavin MacDonaldJason LyeCurt SayreKimberlee Thompson
    • Stephanie Michelle MartinJohn Gavin MacDonaldJason LyeCurt SayreKimberlee Thompson
    • C12M1/34
    • C12Q1/04
    • A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as “addresses”) spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the array provides a distinct spectral response (e.g., pattern of colors) or “fingerprint” for a particular microorganism or class of microorganisms. For example, the array may provide a certain spectral response in the presence of one microorganism or class of microoryanisms (e.g., gram-negative bacteria), but provide a completely different spectral response in the presence of another microorganism or class of microorganisms (e.g., gram-positive bacteria). Detection of the spectral response provided by the array may thus allow for differentiation between microorganisms.
    • 提供了一种用于检测微生物或微生物类别的方法。 更具体地,该方法采用包含在固体支持体上以预定图案间隔开的多个离散区域(称为“地址”)的阵列。 选择地址使得阵列为特定微生物或微生物类提供不同的光谱响应(例如,颜色图案)或“指纹”。 例如,阵列可以在一种微生物或类微生物(例如,革兰氏阴性细菌)的存在下提供特定的光谱响应,但是在另一种微生物或一类微生物的存在下提供完全不同的光谱响应(例如, 革兰氏阳性细菌)。 由阵列提供的光谱响应的检测因此可以允许微生物之间的分化。
    • 6. 发明申请
    • Method for Detecting Candida on Skin
    • 检测皮肤上假丝酵母的方法
    • US20100291670A1
    • 2010-11-18
    • US12843922
    • 2010-07-27
    • Stephanie M. MartinAndrew M. LongJ. Gavin MacDonaldJason Lye
    • Stephanie M. MartinAndrew M. LongJ. Gavin MacDonaldJason Lye
    • C12M1/34
    • A61L15/56G01N2333/245G01N2333/31G01N2333/40
    • A method and system for rapidly detecting Candida on the skin of a host, such as an infant with diaper rash, is provided. The method includes contacting a dermal sample with a colorant that exhibits a certain spectral response (e.g., color change) in the presence of Candida. For example, the colorant may change from a first color to a second color, from colorless to a color, or from a color to colorless. The colorant is typically capable of differentiating between Candida (e.g., Candida albicans) and other microorganisms commonly associated with diaper rash, such as S. aureus and E. coli. Thus, when a dermal sample is placed into contact with the colorant, the color change may simply be observed to determine whether the infection is caused by Candida. If the color change occurs to a certain extent (e.g., from yellow to bright red), it may be determined that the test sample contains Candida. Likewise, if a color change occurs to a lesser extent (e.g., from yellow to faint orange) or not at all, it may be determined that the dermal sample contains other microorganisms (e.g., S. aureus or E. coli), no infection is present, or that the infection is simply due to other causes. Regardless, it will become readily apparent whether or not treatment for Candida is needed.
    • 提供了用于快速检测宿主皮肤上的假丝酵母的方法和系统,例如具有尿布疹的婴儿。 该方法包括使真皮样品与在假丝酵母存在下显示特定光谱响应(例如颜色变化)的着色剂接触。 例如,着色剂可以从第一颜色变为第二颜色,从无色到颜色,或从颜色变为无色。 着色剂通常能够区分假丝酵母(例如白色念珠菌)和通常与尿布疹相关的其它微生物,例如金黄色葡萄球菌和大肠杆菌。 因此,当真皮样品与着色剂接触时,可以简单地观察颜色变化以确定感染是否由念珠菌引起。 如果颜色变化发生一定程度(例如,从黄色到鲜红色),则可以确定测试样品含有念珠菌。 同样地,如果在较小程度上发生颜色变化(例如,从黄色到微弱橙色),或根本不发生变色,则可以确定真皮样品含有其他微生物(例如金黄色葡萄球菌或大肠杆菌),无感染 存在,或感染仅仅是由于其他原因。 无论是否需要对念珠菌的治疗,将变得显而易见。
    • 7. 发明申请
    • RAPID ASSESSMENT OF UPPER RESPIRATORY CONDITIONS
    • 快速评估上呼吸道条件
    • US20090111088A1
    • 2009-04-30
    • US11847569
    • 2007-08-30
    • Stephanie M. MartinJ. Gavin MacDonaldJason LyeCurtis SayreKimberlee Thompson
    • Stephanie M. MartinJ. Gavin MacDonaldJason LyeCurtis SayreKimberlee Thompson
    • C12Q1/70
    • G01N33/53C12Q1/04G01N2333/005G01N2333/195
    • A method for rapidly assessing upper respiratory conditions is provided. More specifically, the method involves contacting a sample obtained from the upper respiratory tract of a host with a test strip. The test strip contains an indicator that provides a broad spectrum response in the presence of bacteria, mold, yeast, or other microorganisms that is different than its response in the presence of viruses. This allows for a rapid and simple assessment as to whether the test sample is infected with a virus or some other microorganism. To help a clinician identify the proper course of treatment, it may also be desirable to obtain further information about the particular type of microorganism present. In this regard, the test strip contains any array of one or more differentiating indicators that provides a certain spectral response in the presence of different types of microorganisms. For example, the array may provide a certain spectral response in the presence of gram-negative bacteria, but a completely different spectral response in the presence of gram-positive bacteria. Likewise, the array may provide a certain spectral response in the presence of Rhinoviruses (associated with the common cold), but a different response in the presence of Influenza viruses. Detection of the spectral response provided by the indicators may thus allow for rapid differentiation between different types of microorganisms.
    • 提供了一种快速评估上呼吸道疾病的方法。 更具体地,该方法包括使从主体上呼吸道获得的样品与测试条接触。 测试条含有指示剂,其在细菌,霉菌,酵母或其它与病毒存在下的反应不同的微生物存在下提供广谱响应。 这允许对测试样品是否感染病毒或其他微生物进行快速和简单的评估。 为了帮助临床医生识别正确的治疗过程,还可能需要获得关于存在的微生物的特定类型的进一步信息。 在这方面,测试条含有任何阵列的一个或多个微分指示剂,在存在不同类型的微生物时提供一定的光谱响应。 例如,阵列可以在存在革兰氏阴性细菌的情况下提供一定的光谱响应,但是在存在革兰氏阳性细菌的情况下是完全不同的光谱响应。 同样,阵列可以在存在鼻病毒(与常见感冒相关))的情况下提供一定的光谱响应,但是在存在流感病毒的情况下可以提供不同的响应。 由指示剂提供的光谱响应的检测因此可以允许不同类型的微生物之间的快速分化。
    • 8. 发明授权
    • Method for detecting Candida on skin
    • 检测皮肤上假丝酵母的方法
    • US08361742B2
    • 2013-01-29
    • US12843922
    • 2010-07-27
    • Stephanie M. MartinAndrew M. Long, IIIJohn Gavin MacDonaldJason Lye
    • Stephanie M. MartinAndrew M. Long, IIIJohn Gavin MacDonaldJason Lye
    • C12Q1/22C12Q1/02
    • A61L15/56G01N2333/245G01N2333/31G01N2333/40
    • A method and system for rapidly detecting Candida on the skin of a host, such as an infant with diaper rash, is provided. The method includes contacting a dermal sample with a colorant that exhibits a certain spectral response (e.g., color change) in the presence of Candida. For example, the colorant may change from a first color to a second color, from colorless to a color, or from a color to colorless. The colorant is typically capable of differentiating between Candida (e.g., Candida albicans) and other microorganisms commonly associated with diaper rash, such as S. aureus and E. coli. Thus, when a dermal sample is placed into contact with the colorant, the color change may simply be observed to determine whether the infection is caused by Candida. If the color change occurs to a certain extent (e.g., from yellow to bright red), it may be determined that the test sample contains Candida. Likewise, if a color change occurs to a lesser extent (e.g., from yellow to faint orange) or not at all, it may be determined that the dermal sample contains other microorganisms (e.g., S. aureus or E. coli), no infection is present, or that the infection is simply due to other causes. Regardless, it will become readily apparent whether or not treatment for Candida is needed.
    • 提供了用于快速检测宿主皮肤上的假丝酵母的方法和系统,例如具有尿布疹的婴儿。 该方法包括使真皮样品与在假丝酵母存在下显示特定光谱响应(例如颜色变化)的着色剂接触。 例如,着色剂可以从第一颜色变为第二颜色,从无色到颜色,或从颜色变为无色。 着色剂通常能够区分假丝酵母(例如白色念珠菌)和通常与尿布疹相关的其它微生物,例如金黄色葡萄球菌和大肠杆菌。 因此,当真皮样品与着色剂接触时,可以简单地观察颜色变化以确定感染是否由念珠菌引起。 如果颜色变化发生一定程度(例如,从黄色到鲜红色),则可以确定测试样品含有念珠菌。 同样地,如果在较小程度上发生颜色变化(例如,从黄色到微弱橙色),或根本不发生变色,则可以确定真皮样品含有其他微生物(例如金黄色葡萄球菌或大肠杆菌),无感染 存在,或感染仅仅是由于其他原因。 无论是否需要对念珠菌的治疗,将变得显而易见。
    • 10. 发明申请
    • Method for detecting Candida on skin
    • 检测皮肤上假丝酵母的方法
    • US20080057532A1
    • 2008-03-06
    • US11513500
    • 2006-08-31
    • Stephanie MartinAndrew M. LongJ. Gavin MacDonaldJason Lye
    • Stephanie MartinAndrew M. LongJ. Gavin MacDonaldJason Lye
    • C12Q1/04
    • A61L15/56G01N2333/245G01N2333/31G01N2333/40
    • A method and system for rapidly detecting Candida on the skin of a host, such as an infant with diaper rash, is provided. The method includes contacting a dermal sample with a colorant that exhibits a certain spectral response (e.g., color change) in the presence of Candida. For example, the colorant may change from a first color to a second color, from colorless to a color, or from a color to colorless. The colorant is typically capable of differentiating between Candida (e.g., Candida albicans) and other microorganisms commonly associated with diaper rash, such as S. aureus and E. coli. Thus, when a dermal sample is placed into contact with the colorant, the color change may simply be observed to determine whether the infection is caused by Candida. If the color change occurs to a certain extent (e.g., from yellow to bright red), it may be determined that the test sample contains Candida. Likewise, if a color change occurs to a lesser extent (e.g., from yellow to faint orange) or not at all, it may be determined that the dermal sample contains other microorganisms (e.g., S. aureus or E. coli), no infection is present, or that the infection is simply due to other causes. Regardless, it will become readily apparent whether or not treatment for Candida is needed.
    • 提供了用于快速检测宿主皮肤上的假丝酵母的方法和系统,例如具有尿布疹的婴儿。 该方法包括使真皮样品与在假丝酵母存在下显示特定光谱响应(例如颜色变化)的着色剂接触。 例如,着色剂可以从第一颜色变为第二颜色,从无色到颜色,或从颜色变为无色。 着色剂通常能够区分假丝酵母(例如白色念珠菌)和通常与尿布疹相关的其它微生物,例如金黄色葡萄球菌和大肠杆菌。 因此,当真皮样品与着色剂接触时,可以简单地观察颜色变化以确定感染是否由念珠菌引起。 如果颜色变化发生一定程度(例如,从黄色到鲜红色),则可以确定测试样品含有念珠菌。 同样地,如果在较小程度上发生颜色变化(例如,从黄色到微弱橙色),或根本不发生变色,则可以确定真皮样品含有其他微生物(例如金黄色葡萄球菌或大肠杆菌),无感染 存在,或感染仅仅是由于其他原因。 无论是否需要对念珠菌的治疗,将变得显而易见。