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1. 发明授权

US08361169B2 Methods for forming dyed microspheres and populations of dyed microspheres 有权
标题翻译：形成染色微球体和染色微球体群体的方法
公开(公告)号：US08361169B2
公开(公告)日：2013-01-29
申请号：US13246486
申请日：2011-09-27
申请人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
发明人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
IPC分类号： C07C247/00
CPC分类号： C09B69/10 , C08F8/00 , C09B67/0097 , D06P1/008 , G01N33/585 , C08F212/08
摘要： Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.
摘要翻译：提供了形成染色微球的各种方法。一种方法包括使用热或光活化与染料偶联的化学结构，以在微球存在下形成反应中间体。反应中间体共价连接到微球的聚合物上，从而将染料偶联到聚合物上并形成染色的微球。提供用于形成耦合到分子的染色微球的其它方法。除了在染色的微球的外表面上合成分子之外，这些方法包括如上所述染色微球。还提供了一批染色微球。群体的每个染色的微球包括通过化学结构连接到每个染色微球的聚合物上的染料。由染料染色的微球体的染料特性的变异系数小于约10％。

2. 发明授权

US08124943B1 Methods and systems for altering fluorescent intensities of a plurality of particles 有权
标题翻译：用于改变多个颗粒的荧光强度的方法和系统
公开(公告)号：US08124943B1
公开(公告)日：2012-02-28
申请号：US11696771
申请日：2007-04-05
申请人： Ananda G. Lugade , Bruce J. C. Bernard , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Bruce J. C. Bernard , Kurt D. Hoffacker
IPC分类号： G01J1/58 , G01N21/64 , G01N15/02 , G01N21/00
CPC分类号： G01N15/1456 , G01N2021/6439
摘要： A method for altering fluorescent emissions of particles includes setting the particles in motion and exposing the moving particles to light such that fluorescent intensities of the particles are lessened isotropically and substantially simultaneously. Another method includes measuring fluorescent emissions of particles, determining the measured fluorescent emissions do not collectively fit within a first predetermined range of fluorescent values, and exposing the particles to one or more incidents of light that are configured to cooperatively alter the fluorescent emissions of the particles to be within a second predetermined range of fluorescent values. An embodiment of an apparatus includes a vessel configured to contain a plurality of particles and a means for setting the particles in motion. The apparatus further includes an illumination subsystem configured to direct light toward the vessel and at a spectral window (i.e., wavelength or band of wavelengths) which is configured to isotropically and substantially simultaneously lessen the fluorescent emissions of each of the particles.
摘要翻译：用于改变颗粒的荧光发射的方法包括使颗粒运动并使可移动的颗粒暴露于光，使得颗粒的荧光强度各向同性地并且基本上同时减少。另一种方法包括测量颗粒的荧光发射，确定所测量的荧光发射不共同适合于荧光值的第一预定范围内，并将颗粒暴露于被配置为协调地改变颗粒的荧光发射的一种或多种光的事件在荧光值的第二预定范围内。装置的实施例包括构造成容纳多个颗粒的容器和用于设置颗粒运动的装置。该装置还包括被配置为将光引向容器并且被配置为各向同性地并且基本上同时减少每个颗粒的荧光发射的光谱窗（即波长或波长带）的照明子系统。

3. 发明授权

US08088629B1 Methods for forming dyed microspheres and populations of microspheres 有权
标题翻译：形成染色微球体和微球体的方法
公开(公告)号：US08088629B1
公开(公告)日：2012-01-03
申请号：US11248039
申请日：2005-10-12
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： C08L101/00 , C09K3/00
CPC分类号： C09B67/0097 , C09B67/0013 , Y10T436/25 , Y10T436/255
摘要： Various methods for forming dyed microspheres are provided. One method includes attaching a hydrophilic dye to chemical groups to form a bubble. The bubble includes the chemical groups surrounding the hydrophilic dye and an aqueous solution. The chemical groups are soluble in aqueous and organic solvents. The method also includes disposing the bubble and a microsphere to be dyed in a solvent such that the bubble is incorporated into the microsphere thereby dyeing the microsphere. Another method includes adsorbing a hydrophilic dye on a surface of a hydrophobic polymer core of a microsphere to be dyed thereby dyeing the microsphere. The method also includes attaching chemical groups to the hydrophilic dye. The chemical groups are soluble in aqueous and organic solvents. The chemical groups attached to the hydrophilic dye form an enclosure surrounding the hydrophilic dye and an aqueous solution.
摘要翻译：提供了形成染色微球的各种方法。一种方法包括将亲水染料连接到化学基团以形成气泡。气泡包括亲水染料周围的化学基团和水溶液。化学基团可溶于水和有机溶剂。该方法还包括将泡沫和待染色的微球体置于溶剂中，使得气泡加入到微球体中，从而染色微球体。另一种方法包括将亲水染料吸附在待染色的微球的疏水聚合物芯的表面上，从而染色微球。该方法还包括将化学基团连接到亲水性染料上。化学基团可溶于水和有机溶剂。附着于亲水染料的化学基团形成围绕亲水性染料和水溶液的封闭物。

4. 发明授权

US07867774B2 Methods for altering surface characteristics of microspheres 有权
标题翻译：改变微球表面特性的方法
公开(公告)号：US07867774B2
公开(公告)日：2011-01-11
申请号：US11247943
申请日：2005-10-11
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： G01N21/75 , G01N37/00 , A61K9/16
CPC分类号： G01N21/645 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/586 , G01N2021/6421 , G01N2650/00 , Y10T436/13
摘要： Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.
摘要翻译：提供了改变微球表面特性的各种方法。一种方法包括将烯醇酸偶联到微球以改变微球的表面特性。表面特征可以包括电荷密度和/或pKa。可以通过烯醇酸将试剂偶联到微球。试剂可以包括生物分子。当试剂与微球耦合时，改性表面特性可增加试剂的稳定性。改进的表面特性还可以改善用微球进行的分析的性能。另一个实施方案涉及包含与微球的聚合物核心偶联的烯醇酸的微球，使得烯醇酸改性微球的表面特性。可以通过烯醇酸将试剂偶联到微球。

5. 发明申请

US20120123026A1 FUNCTIONALIZED COMPOSITIONS FOR IMPROVED IMMOBILIZATION 有权
标题翻译：功能化组合物，用于改善固化
公开(公告)号：US20120123026A1
公开(公告)日：2012-05-17
申请号：US12119203
申请日：2008-05-12
申请人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
发明人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
IPC分类号： C08F212/36 , C08K9/12 , C08H1/00 , B82Y5/00
CPC分类号： B82Y30/00 , C07B2200/11 , C07C243/28 , C07C303/22 , C07C303/32 , C07C315/04 , C07C319/18 , C07C319/22 , C07C381/02 , C07C2601/04 , C07D239/48 , C07K1/042 , C07K17/00 , G01N33/54353 , Y10S977/902 , C07C309/47 , C07C309/88 , C07C309/46 , C07C317/18 , C07C323/65 , C07C323/42 , C07C309/42
摘要： The present invention relates to improved covalent coupling of two or more entities such as biomolecules, polymer compositions, organic/inorganic molecules/materials, and the like, including their combinations, through one or more novel reactive groups attached to linker groups of 2-1000 atoms length. The present invention also contemplates the use of bifunctional bridge molecules to link two or more entities, wherein the functional groups of the bridge molecules are the novel reactive groups of the present invention.
摘要翻译：本发明涉及两个或多个实体如生物分子，聚合物组合物，有机/无机分子/材料等的改进的共价偶联，包括其组合，通过连接到2-1000的连接基团上的一个或多个新的反应性基团原子长度。本发明还考虑使用双官能桥分子连接两个或多个实体，其中桥分子的官能团是本发明的新的反应性基团。

6. 发明授权

US08153440B2 Methods for altering surface characteristics of microspheres 有权
标题翻译：改变微球表面特性的方法
公开(公告)号：US08153440B2
公开(公告)日：2012-04-10
申请号：US12969863
申请日：2010-12-16
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： G01N21/75 , G01N37/00 , A61K9/16
CPC分类号： G01N21/645 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/586 , G01N2021/6421 , G01N2650/00 , Y10T436/13
摘要： Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.
摘要翻译：提供了改变微球表面特性的各种方法。一种方法包括将烯醇酸偶联到微球以改变微球的表面特性。表面特征可以包括电荷密度和/或pKa。可以通过烯醇酸将试剂偶联到微球。试剂可以包括生物分子。当试剂与微球耦合时，改性表面特性可增加试剂的稳定性。改进的表面特性还可以改善用微球进行的分析的性能。另一个实施方案涉及包含与微球的聚合物核心偶联的烯醇酸的微球，使得烯醇酸改性微球的表面特性。可以通过烯醇酸将试剂偶联到微球。

7. 发明申请

US20120035328A1 METHODS FOR FORMING DYED MICROSPHERES AND POPULATIONS OF DYED MICROSPHERES 有权
标题翻译：用于形成染色微球的人造粒的方法
公开(公告)号：US20120035328A1
公开(公告)日：2012-02-09
申请号：US13246486
申请日：2011-09-27
申请人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
发明人： Kurt D. Hoffacker , Ananda G. Lugade , Ewald Terpetschnig
IPC分类号： C08F12/30 , C07D403/08 , C07C311/49
CPC分类号： C09B69/10 , C08F8/00 , C09B67/0097 , D06P1/008 , G01N33/585 , C08F212/08
摘要： Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.
摘要翻译：提供了形成染色微球的各种方法。一种方法包括使用热或光活化与染料偶联的化学结构，以在微球存在下形成反应中间体。反应中间体共价连接到微球的聚合物上，从而将染料偶联到聚合物上并形成染色的微球。提供用于形成耦合到分子的染色微球的其它方法。除了在染色的微球的外表面上合成分子之外，这些方法包括如上所述染色微球。还提供了一批染色微球。群体的每个染色的微球包括通过化学结构连接到每个染色微球的聚合物上的染料。由染料染色的微球体的染料特性的变异系数小于约10％。

8. 发明申请

US20110086431A1 Methods for Altering Surface Characteristics of Microspheres 有权
标题翻译：改变微球表面特性的方法
公开(公告)号：US20110086431A1
公开(公告)日：2011-04-14
申请号：US12969863
申请日：2010-12-16
申请人： Ananda G. Lugade , Kurt D. Hoffacker
发明人： Ananda G. Lugade , Kurt D. Hoffacker
IPC分类号： G01N21/75
CPC分类号： G01N21/645 , G01N33/54313 , G01N33/54353 , G01N33/54393 , G01N33/586 , G01N2021/6421 , G01N2650/00 , Y10T436/13
摘要： Various methods for altering surface characteristics of a microsphere are provided. One method includes coupling an enolic acid to the microsphere to modify the surface characteristics of the microsphere. The surface characteristics may include charge density and/or pKa. A reagent can be coupled to the microsphere via the enolic acid. The reagent may include a biomolecule. The modified surface characteristics may increase a stability of the reagent when the reagent is coupled to the microsphere. The modified surface characteristics may also improve performance of an assay carried out with the microsphere. Another embodiment relates to a microsphere that includes an enolic acid coupled to a polymer core of the microsphere such that the enolic acid modifies surface characteristics of the microsphere. A reagent can be coupled to the microsphere via the enolic acid.
摘要翻译：提供了改变微球表面特性的各种方法。一种方法包括将烯醇酸偶联到微球以改变微球的表面特性。表面特征可以包括电荷密度和/或pKa。可以通过烯醇酸将试剂偶联到微球。试剂可以包括生物分子。当试剂与微球耦合时，改性表面特性可增加试剂的稳定性。改进的表面特性还可以改善用微球进行的分析的性能。另一个实施方案涉及包含与微球的聚合物核心偶联的烯醇酸的微球，使得烯醇酸改性微球的表面特性。可以通过烯醇酸将试剂偶联到微球。

9. 发明申请

US20080103061A1 FUNCTIONALIZED COMPOSITIONS FOR IMPROVED IMMOBILIZATION 有权
标题翻译：功能化组合物，用于改善固化
公开(公告)号：US20080103061A1
公开(公告)日：2008-05-01
申请号：US11621209
申请日：2007-01-09
申请人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
发明人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
IPC分类号： C40B40/04 , C40B40/06 , C40B40/10
CPC分类号： B82Y30/00 , C07B2200/11 , C07C243/28 , C07C303/22 , C07C303/32 , C07C315/04 , C07C319/18 , C07C319/22 , C07C381/02 , C07C2601/04 , C07D239/48 , C07K1/042 , C07K17/00 , G01N33/54353 , Y10S977/902 , C07C309/47 , C07C309/88 , C07C309/46 , C07C317/18 , C07C323/65 , C07C323/42 , C07C309/42
摘要： The present invention relates to improved covalent coupling of two or more entities such as biomolecules, polymer compositions, organic/inorganic molecules/materials, and the like, including their combinations, through one or more novel reactive groups attached to linker groups of 2-1000 atoms length. The present invention also contemplates the use of bifunctional bridge molecules to link two or more entities, wherein the functional groups of the bridge molecules are the novel reactive groups of the present invention.
摘要翻译：本发明涉及两个或多个实体如生物分子，聚合物组合物，有机/无机分子/材料等的改进的共价偶联，包括其组合，通过连接到2-1000的连接基团上的一个或多个新的反应性基团原子长度。本发明还考虑使用双官能桥分子连接两个或多个实体，其中桥分子的官能团是本发明的新的反应性基团。

10. 发明授权

US08188269B1 Functionalized compositions for improved immobilization 有权
标题翻译：用于改善固定化的官能化组合物
公开(公告)号：US08188269B1
公开(公告)日：2012-05-29
申请号：US12119203
申请日：2008-05-12
申请人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
发明人： Ananda G. Lugade , Kurt D. Hoffacker , Adam J. Jenkins , Karri L. Michael-Ballard , Leonid Patsenker , Ewald Terpetschnig , Veronica D. Thomason , Ralph McDade
IPC分类号： C07D201/00
CPC分类号： B82Y30/00 , C07B2200/11 , C07C243/28 , C07C303/22 , C07C303/32 , C07C315/04 , C07C319/18 , C07C319/22 , C07C381/02 , C07C2601/04 , C07D239/48 , C07K1/042 , C07K17/00 , G01N33/54353 , Y10S977/902 , C07C309/47 , C07C309/88 , C07C309/46 , C07C317/18 , C07C323/65 , C07C323/42 , C07C309/42
摘要： The present invention relates to improved covalent coupling of two or more entities such as biomolecules, polymer compositions, organic/inorganic molecules/materials, and the like, including their combinations, through one or more novel reactive groups attached to linker groups of 2-1000 atoms length. The present invention also contemplates the use of bifunctional bridge molecules to link two or more entities, wherein the functional groups of the bridge molecules are the novel reactive groups of the present invention.
摘要翻译：本发明涉及两个或多个实体如生物分子，聚合物组合物，有机/无机分子/材料等的改进的共价偶联，包括其组合，通过连接到2-1000的连接基团上的一个或多个新的反应性基团原子长度。本发明还考虑使用双官能桥分子连接两个或多个实体，其中桥分子的官能团是本发明的新的反应性基团。

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