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1. 发明授权

US07823470B2 Cantilever and cantilever manufacturing method 有权
标题翻译：悬臂和悬臂制造方法
公开(公告)号：US07823470B2
公开(公告)日：2010-11-02
申请号：US11709495
申请日：2007-02-22
申请人： Masatsugu Shigeno , Osamu Matsuzawa , Naoya Watanabe , Amiko Nihei , Akira Inoue , Yoshiharu Shirakawabe , Hiroshi Muramatsu , Yuji Yamamoto
发明人： Masatsugu Shigeno , Osamu Matsuzawa , Naoya Watanabe , Amiko Nihei , Akira Inoue , Yoshiharu Shirakawabe , Hiroshi Muramatsu , Yuji Yamamoto
IPC分类号： G01Q70/00 , G01Q70/10 , G01B21/30
CPC分类号： G01R1/06738 , B33Y80/00 , G01Q70/10 , G01Q70/16 , G01R1/06727 , G01R1/07342 , G01R3/00 , Y10T29/49007
摘要： A cantilever includes: a lever portion; a holder portion supporting the proximal end of the lever portion; a probe portion arranged at the distal end of the lever portion and having a spherical surface to face a sample; and a retaining portion fixed to the distal end of the lever portion and retaining the probe portion to surround a periphery of the probe portion. There is provided a cantilever allowing mounting of a probe portion with little effect in a short time without using any adhesive.
摘要翻译：悬臂包括：杠杆部分; 支撑杆部的近端的保持部; 探针部分，布置在所述杆部分的远端并且具有面对样品的球形表面; 以及保持部，其固定到所述杆部的前端部，并且保持所述探针部包围所述探针部的周边。提供了一种悬臂，允许在短时间内几乎没有使用任何粘合剂的情况下安装探针部分。

2. 发明授权

US07456400B2 Scanning probe microscope and scanning method 有权
标题翻译：扫描探针显微镜和扫描方法
公开(公告)号：US07456400B2
公开(公告)日：2008-11-25
申请号：US11235458
申请日：2005-09-26
申请人： Masatsugu Shigeno , Yoshiharu Shirakawabe , Amiko Nihei , Osamu Matsuzawa , Naoya Watanabe , Akira Inoue
发明人： Masatsugu Shigeno , Yoshiharu Shirakawabe , Amiko Nihei , Osamu Matsuzawa , Naoya Watanabe , Akira Inoue
IPC分类号： G12B21/00
CPC分类号： G01Q60/32 , G01Q10/06 , Y10S977/849 , Y10S977/851 , Y10S977/852 , Y10S977/86 , Y10S977/872
摘要： A scanning probe microscope has a probe needle and a control section that controls relative scanning movement between the probe needle and a surface of a sample in at least one direction parallel to the sample surface and controls relative movement between the probe needle and the sample surface in a direction perpendicular to the sample surface. A vibration source vibrates the probe needle at a vibrating frequency relative to the sample surface. An approach/separation drive section causes the probe needle to relatively approach to and separate from the sample surface at a predetermined distance while the probe needle is vibrated at the vibrating frequency relative to the sample surface by the vibration source. A detection section detects a rate of change in a vibration state of the probe needle in accordance with a distance between the probe needle and the sample surface. An observation section gathers observation data from the sample surface when the rate of change in the vibration state of the probe needle detected by the detection section has exceeded a preselected threshold value.
摘要翻译：扫描探针显微镜具有探针和控制部，其控制探针和样品表面之间的平行于样品表面的至少一个方向上的相对扫描运动，并且控制探针与样品表面之间的相对运动垂直于样品表面的方向。振动源以相对于样品表面的振动频率振动探针。接近/分离驱动部分使得探针相对于样品表面以预定距离相对接近和分离，同时探针以振动源相对于样品表面的振动频率振动。检测部根据探针与样本面的距离来检测探针的振动状态的变化率。当由检测部检测到的探针的振动状态的变化率超过预选阈值时，观察部分从样本表面收集观察数据。

3. 发明授权

US08859279B2 Cell detachment method 有权
标题翻译：细胞分离法
公开(公告)号：US08859279B2
公开(公告)日：2014-10-14
申请号：US11810768
申请日：2007-06-07
申请人： Amiko Nihei , Masatsugu Shigeno , Yoshiharu Shirakawabe , Akira Inoue , Osamu Matsuzawa , Naoya Watanabe
发明人： Amiko Nihei , Masatsugu Shigeno , Yoshiharu Shirakawabe , Akira Inoue , Osamu Matsuzawa , Naoya Watanabe
IPC分类号： C12N5/00 , C12M1/00
CPC分类号： C12M47/04 , C12M33/00
摘要： A cell detachment method for detaching only a desired cell from a plurality of cells cultured on a substrate under predetermined culture environment conditions by using a scanning probe microscope having a probe, comprising: observing the plural cells; specifying the cell to be detached; moving the probe onto the specified cell; and pressing the prove against the specified cell with a predetermined force so as to detach the cell from the substrate.
摘要翻译：一种细胞分离方法，其通过使用具有探针的扫描探针显微镜，在预定的培养环境条件下从仅在基质上培养的多个细胞中仅去除所需细胞，包括：观察所述多个细胞; 指定要分离的单元格; 将探针移动到指定的细胞上; 并用预定的力将证明压在指定的电池上，以将电池与基板分离。

4. 发明申请

US20070292946A1 Cell detachment method 有权
标题翻译：细胞分离法
公开(公告)号：US20070292946A1
公开(公告)日：2007-12-20
申请号：US11810768
申请日：2007-06-07
申请人： Amiko Nihei , Masatsugu Shigeno , Yoshiharu Shirakawabe , Akira Inoue , Osamu Matsuzawa , Naoya Watanabe
发明人： Amiko Nihei , Masatsugu Shigeno , Yoshiharu Shirakawabe , Akira Inoue , Osamu Matsuzawa , Naoya Watanabe
IPC分类号： C12M3/00 , C12N5/06
CPC分类号： C12M47/04 , C12M33/00
摘要： A cell detachment method for detaching only a desired cell from a plurality of cells cultured on a substrate under predetermined culture environment conditions by using a scanning probe microscope having a probe, comprising: observing the plural cells; specifying the cell to be detached; moving the probe onto the specified cell; and pressing the prove against the specified cell with a predetermined force so as to detach the cell from the substrate.
摘要翻译：一种细胞分离方法，其通过使用具有探针的扫描探针显微镜，在预定的培养环境条件下从仅在基质上培养的多个细胞中仅去除所需细胞，包括：观察所述多个细胞; 指定要分离的单元格; 将探针移动到指定的细胞上; 并用预定的力将证明压在指定的电池上，以将电池与基板分离。

5. 发明申请

US20070214875A1 Cantilever and cantilever manufacturing method 有权
标题翻译：悬臂和悬臂制造方法
公开(公告)号：US20070214875A1
公开(公告)日：2007-09-20
申请号：US11709495
申请日：2007-02-22
申请人： Masatsugu Shigeno , Osamu Matsuzawa , Naoya Watanabe , Amiko Nihei , Akira Inoue , Yoshiharu Shirakawabe , Hiroshi Muramatsu , Yuji Yamamoto
发明人： Masatsugu Shigeno , Osamu Matsuzawa , Naoya Watanabe , Amiko Nihei , Akira Inoue , Yoshiharu Shirakawabe , Hiroshi Muramatsu , Yuji Yamamoto
IPC分类号： G01B21/30 , G01R3/00 , H01L21/306 , G12B21/02
CPC分类号： G01R1/06738 , B33Y80/00 , G01Q70/10 , G01Q70/16 , G01R1/06727 , G01R1/07342 , G01R3/00 , Y10T29/49007
摘要： A cantilever includes: a lever portion; a holder portion supporting the proximal end of the lever portion; a probe portion arranged at the distal end of the lever portion and having a spherical surface to face a sample; and a retaining portion fixed to the distal end of the lever portion and retaining the probe portion to surround a periphery of the probe portion. There is provided a cantilever allowing mounting of a probe portion with little effect in a short time without using any adhesive.
摘要翻译：悬臂包括：杠杆部分; 支撑杆部的近端的保持部; 探针部分，布置在所述杆部分的远端并且具有面对样品的球形表面; 以及保持部，其固定到所述杆部的前端部，并且保持所述探针部包围所述探针部的周边。提供了一种悬臂，允许在短时间内几乎没有使用任何粘合剂的情况下安装探针部分。

6. 发明申请

US20060113472A1 Scanning probe microscope and scanning method 有权
标题翻译：扫描探针显微镜和扫描方法
公开(公告)号：US20060113472A1
公开(公告)日：2006-06-01
申请号：US11235458
申请日：2005-09-26
申请人： Masatsugu Shigeno , Yoshiharu Shirakawabe , Amiko Nihei , Osamu Matsuzawa , Naoya Watanabe , Akira Inoue
发明人： Masatsugu Shigeno , Yoshiharu Shirakawabe , Amiko Nihei , Osamu Matsuzawa , Naoya Watanabe , Akira Inoue
IPC分类号： G21K7/00
CPC分类号： G01Q60/32 , G01Q10/06 , Y10S977/849 , Y10S977/851 , Y10S977/852 , Y10S977/86 , Y10S977/872
摘要： In order to provide a scanning probe microscope and a scanning method which are capable of accurately approaching or contacting a probe needle and a sample surface irrespective of an irregularities shape of the sample surface, it comprises a probe needle 2 for relatively performing, with respect to a sample surface S, scans in two directions parallel to the sample surface S and a movement in a perpendicular direction of the sample surface S, a detection means 4 for detecting a measurement amount changing in compliance with a distance between the probe needle 2 and the sample surface S, an observation means 6 for gathering an observation data in a point of time at which the probe needle 2 has approached to or contacted with the sample surface S, a control means 5 for controlling the sans in the two directions and the movement in the perpendicular direction and an approach/separation drive section 24 for causing the probe needle 2 to relatively approach to and separate from the sample surface S at a predetermined distance, wherein the detection means 4 detects a change rate of the measurement amount at an approach time and a separation time by the approach/separation drive section 24, and the observation means 6 gathers the observation data when the change rate of the observation amount exceeds a previously set threshold value.
摘要翻译：为了提供能够精确地接近或接触探针和样品表面的扫描探针显微镜和扫描方法，而与样品表面的不规则形状无关，其包括用于相对于相对于样品表面S在平行于样品表面S的两个方向上扫描并沿着样品表面S的垂直方向移动;检测装置4，用于检测测量量根据探针2和样本表面S，用于在探针2接近或接触样本表面S的时间点收集观察数据的观察装置6，用于控制两个方向上的无效的控制装置5和运动以及用于使探针2相对于样品su相对接近和分离的接近/分离驱动部24 表面S在预定距离处，其中检测装置4通过接近/分离驱动部分24检测接近时间的测量量和分离时间的变化率，并且观察装置6在变化率的观察量超过预先设定的阈值。

7. 发明申请

US20090265819A1 SENSOR FOR OBSERVATIONS IN LIQUID ENVIRONMENTS AND OBSERVATION APPARATUS FOR USE IN LIQUID ENVIRONMENTS 有权
标题翻译：用于液体环境观测的传感器和用于液体环境的观察装置
公开(公告)号：US20090265819A1
公开(公告)日：2009-10-22
申请号：US12416634
申请日：2009-04-01
申请人： Naoya Watanabe , Masatsugu Shigeno , Masato Iyoki
发明人： Naoya Watanabe , Masatsugu Shigeno , Masato Iyoki
IPC分类号： G01N13/10
CPC分类号： G01Q20/04 , G01Q30/14
摘要： The sensor has the self-detecting probe including a body portion, an elongated belt-like flexible substrate, connecting members, a resinous portion, and external contacts formed at the ends of the flexible substrate brought out of liquid. The probe further includes a cantilever whose base end is supported to the body portion, a strain resistive element whose resistance value varies according to the amount of displacement of the cantilever, and interconnects electrically connected with the strain resistive element. A probe tip is formed at the front end of the cantilever. The flexible substrate has an interconnect pattern sandwiched between two insulating sheets. The flexible substrate supports the body portion while the cantilever protrudes outwardly. At least one end of the flexible substrate is brought out of liquid. The connecting members connect the interconnects with the interconnect pattern. The interconnects, connecting members, and the portions of the connecting members electrically connected with the interconnect pattern are coated with the resinous portion.
摘要翻译：传感器具有包括主体部分，细长带状柔性基板，连接部件，树脂部分和形成在柔性基板的端部的外部触点的自检探测器。探头还包括一个悬臂，其基端支撑在主体部分，一个应变阻力元件，其电阻值根据悬臂的位移量而变化，并且与应变电阻元件电连接。探针尖端形成在悬臂的前端。柔性基板具有夹在两个绝缘片之间的互连图案。柔性基板支撑主体部分，而悬臂向外突出。将柔性基板的至少一端从液体中取出。连接构件将互连件与互连图案连接。互连件，连接件以及与互连图形电连接的连接件的部分涂覆有树脂部分。

8. 发明授权

US08214915B2 Cantilever, cantilever system, scanning probe microscope, mass sensor apparatus, viscoelasticity measuring instrument, manipulation apparatus, displacement determination method of cantilever, vibration method of cantilever and deformation method of cantilever 有权
标题翻译：悬臂，悬臂系统，扫描探针显微镜，质量传感器装置，粘弹性测量仪器，操纵装置，悬臂位移测定方法，悬臂振动方法及悬臂变形方法
公开(公告)号：US08214915B2
公开(公告)日：2012-07-03
申请号：US12455556
申请日：2009-06-03
申请人： Masatsugu Shigeno , Kazutoshi Watanabe , Masato Iyoki , Naoya Watanabe
发明人： Masatsugu Shigeno , Kazutoshi Watanabe , Masato Iyoki , Naoya Watanabe
IPC分类号： G01Q20/00
CPC分类号： G01Q70/14 , G01G3/12 , G01G9/00 , G01Q10/045 , G01Q60/32
摘要： Provided is a cantilever that is capable of bending and deforming in an active manner by itself. The cantilever includes: a lever portion having a proximal end that is supported by a main body part; and a resistor member that is formed in the cantilever and generates heat when a voltage is applied, to thereby deform the lever portion by thermal expansion due to the heat.
摘要翻译：本发明提供一种能够以活跃的方式自身弯曲和变形的悬臂。悬臂包括：杠杆部分，其具有由主体部分支撑的近端; 以及形成在悬臂中并在施加电压时产生热量的电阻器构件，从而通过由于热量的热膨胀而使杆部变形。

9. 发明授权

US07945965B2 Sensor for observations in liquid environments and observation apparatus for use in liquid environments 有权
标题翻译：用于液体环境中观察的传感器和用于液体环境的观察设备
公开(公告)号：US07945965B2
公开(公告)日：2011-05-17
申请号：US12416634
申请日：2009-04-01
申请人： Naoya Watanabe , Masatsugu Shigeno , Masato Iyoki
发明人： Naoya Watanabe , Masatsugu Shigeno , Masato Iyoki
IPC分类号： H01J37/252
CPC分类号： G01Q20/04 , G01Q30/14
摘要： The sensor has the self-detecting probe including a body portion, an elongated belt-like flexible substrate, connecting members, a resinous portion, and external contacts formed at the ends of the flexible substrate brought out of liquid. The probe further includes a cantilever whose base end is supported to the body portion, a strain resistive element whose resistance value varies according to the amount of displacement of the cantilever, and interconnects electrically connected with the strain resistive element. A probe tip is formed at the front end of the cantilever. The flexible substrate has an interconnect pattern sandwiched between two insulating sheets. The flexible substrate supports the body portion while the cantilever protrudes outwardly. At least one end of the flexible substrate is brought out of liquid. The connecting members connect the interconnects with the interconnect pattern. The interconnects, connecting members, and the portions of the connecting members electrically connected with the interconnect pattern are coated with the resinous portion.
摘要翻译：传感器具有包括主体部分，细长带状柔性基板，连接部件，树脂部分和形成在柔性基板的端部的外部触点的自检探测器。探头还包括一个悬臂，其基端支撑在主体部分，一个应变阻力元件，其电阻值根据悬臂的位移量而变化，并且与应变电阻元件电连接。探针尖端形成在悬臂的前端。柔性基板具有夹在两个绝缘片之间的互连图案。柔性基板支撑主体部分，而悬臂向外突出。将柔性基板的至少一端从液体中取出。连接构件将互连件与互连图案连接。互连件，连接件以及与互连图形电连接的连接件的部分涂覆有树脂部分。

10. 发明申请

US20100107284A1 Cantilever, cantilever system, scanning probe microscope, mass sensor apparatus, viscoelasticity measuring instrument, manipulation apparatus, displacement determination method of cantilever, vibration method of cantilever and deformation method of cantilever 有权
标题翻译：悬臂，悬臂系统，扫描探针显微镜，质量传感器装置，粘弹性测量仪器，操纵装置，悬臂位移测定方法，悬臂振动方法及悬臂变形方法
公开(公告)号：US20100107284A1
公开(公告)日：2010-04-29
申请号：US12455556
申请日：2009-06-03
申请人： Masatsugu Shigeno , Kazutoshi Watanabe , Masato Iyoki , Naoya Watanabe
发明人： Masatsugu Shigeno , Kazutoshi Watanabe , Masato Iyoki , Naoya Watanabe
IPC分类号： G01Q20/00 , G01Q70/08 , G01G3/16
CPC分类号： G01Q70/14 , G01G3/12 , G01G9/00 , G01Q10/045 , G01Q60/32
摘要： Provided is a cantilever that is capable of bending and deforming in an active manner by itself. The cantilever includes: a lever portion having a proximal end that is supported by a main body part; and a resistor member that is formed in the cantilever and generates heat when a voltage is applied, to thereby deform the lever portion by thermal expansion due to the heat.
摘要翻译：本发明提供一种能够以活跃的方式自身弯曲和变形的悬臂。悬臂包括：杠杆部分，其具有由主体部分支撑的近端; 以及形成在悬臂中并在施加电压时产生热量的电阻器构件，从而通过由于热量的热膨胀而使杆部变形。

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