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1. 发明授权

US5192675A Cloned KpnI restriction-modification system 失效
标题翻译：克隆KpnI限制修饰系统
公开(公告)号：US5192675A
公开(公告)日：1993-03-09
申请号：US822047
申请日：1992-01-17
申请人： Deb K. Chatterjee , Alan W. Hammond
发明人： Deb K. Chatterjee , Alan W. Hammond
IPC分类号： C12N9/10 , C12N9/22 , C12N15/55
CPC分类号： C12N9/1007 , C12N9/22
摘要： The present invention discloses the cloning and expression in a host such as Escherichia coli of the KpnI restriction-modification system from Klebsiella pneumoniae, utilizing a two step protocol. Initial protection of the E. coli host with methylase expressed on a vector was required to stabilize a compatible vector carrying both the endonuclease and the methylase genes on a single DNA fragment. A chromosomal map was generated localizing the genes for KpnI methylase and endonuclease. An E. coli strain was constructed which produced several thousand-fold higher levels of KpnI endonuclease than the level produced by Klebsiella pneumoniae. This invention is also directed to cloning and expression of genes encoding for restriction endonuclease isoschizomers of KpnI and/or modification methylase isoschizomers of KpnI methylase.
摘要翻译：本发明公开了利用两步方案克隆和表达来自肺炎克雷伯杆菌的KpnI限制性修饰系统的宿主如大肠杆菌。需要用载体上表达的甲基化酶对大肠杆菌宿主进行初步保护，以在同一个DNA片段上稳定携带内切核酸酶和甲基化酶基因的相容载体。产生了定位KpnI甲基化酶和内切核酸酶的基因的染色体图谱。构建大肠杆菌菌株，其产生比肺炎克雷伯杆菌产生的水平高出几千倍的KpnI内切核酸酶。本发明还涉及克隆和表达编码KpnI和/或KpnI甲基化酶的修饰甲基化酶异构体的限制性内切核酸酶异构体的基因。

2. 发明授权

US5147800A Host expressing NgoAIII restriction endonuclease and modification methylase from neisseria 失效
标题翻译：主要表达NGOAIII限制性内切酶和修饰来自NEISSERIA的甲基化酶
公开(公告)号：US5147800A
公开(公告)日：1992-09-15
申请号：US535021
申请日：1990-06-08
申请人： Alan W. Hammond , Deb K. Chatterjee
发明人： Alan W. Hammond , Deb K. Chatterjee
IPC分类号： C12N1/21 , C12N9/10 , C12N9/22 , C12N15/09 , C12R1/36
CPC分类号： C12N9/1007 , C12N9/22 , Y10S435/871
摘要： The present invention is directed to recombinant hosts which contain and express various Type II restriction endonuclease and/or modification methylase genes. In particular, the present invention is concerned with the cloned restriction endonucleases, NgoAIII and NgoAI, which recognize and cleave within or near the double-stranded DNA sequence, 5' CCGCGG 3' and 5' PuGCGCPy 3', respectively. Also provided in this invention are cloned modification methylase genes corresponding to said restriction endonucleases. This invention is further concerned with a cloned modification methylase, M.NgoAII. One source of these enzymes is Neisseria gonorrhoeae, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.
摘要翻译：本发明涉及含有并表达各种II型限制性内切核酸酶和/或修饰甲基化酶基因的重组宿主。特别地，本发明涉及克隆的限制性内切核酸酶NgoAIII和NgoAI，其分别在双链DNA序列内或其附近识别和切割5'CCGCGG 3'和5'PuGCGCPy 3'。本发明还提供了与所述限制性内切核酸酶相应的克隆的修饰甲基化酶基因。本发明还涉及克隆的修饰甲基化酶M.NgoAII。这些酶的一个来源是淋病奈瑟氏球菌，尽管其他微生物可用于分离本发明的限制性内切核酸酶异构体和修饰甲基化酶同位异构体。

3. 发明授权

US5082784A Cloned KpnI restriction-modification system 失效
标题翻译：克隆KPNI限制修改系统
公开(公告)号：US5082784A
公开(公告)日：1992-01-21
申请号：US496283
申请日：1990-03-20
申请人： Deb K. Chatterjee , Alan W. Hammond
发明人： Deb K. Chatterjee , Alan W. Hammond
IPC分类号： C12N1/21 , C12N9/10 , C12N9/22 , C12N15/09 , C12N15/15 , C12N15/55 , C12R1/19 , C12R1/22
CPC分类号： C12N9/1007 , C12N9/22
摘要： The present invention discloses the cloning and expression in a host such as Escherichia coli of the KpnI restriction-modification system from Klebsiella pneumoniae, utilizing a two step protocol. Initial protection of the E. coli host with methylase expressed on a vector was required to stabilize a compatible vector carrying both the endonuclease and the methylase genes on a single DNA fragment. A chromosomal map was generated localizing the genes for KpnI methylase and endonuclease. An E. coli strain was constructed which produced several thousand-fold higher levels of KpnI endonuclease than the level produced by Klebsiella pneumoniae. This invention is also directed to cloning and expression of genes encoding for restriction endonuclease isoschizomers of KpnI and/or modification methylase isoschizomers of KpnI methylase.
摘要翻译：本发明公开了利用两步方案克隆和表达来自肺炎克雷伯杆菌的KpnI限制性修饰系统的宿主如大肠杆菌。需要用载体上表达的甲基化酶对大肠杆菌宿主进行初步保护，以在同一个DNA片段上稳定携带内切核酸酶和甲基化酶基因的相容载体。产生了定位KpnI甲基化酶和内切核酸酶的基因的染色体图谱。构建大肠杆菌菌株，其产生比肺炎克雷伯杆菌产生的水平高出几千倍的KpnI内切核酸酶。本发明还涉及克隆和表达编码KpnI和/或KpnI甲基化酶的修饰甲基化酶异构体的限制性内切核酸酶异构体的基因。

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