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1. 发明授权

US06346402B1 (S)-&agr;-phenethylamine: pyruvate transaminase 失效
标题翻译：（S）-α-苯乙胺：丙酮酸转氨酶
公开(公告)号：US06346402B1
公开(公告)日：2002-02-12
申请号：US09582764
申请日：2000-06-30
申请人： Akira Iwasaki , Yukio Yamada , Yasuhiro Ikenaka , Yoshihiko Yasohara , Junzo Hasegawa
发明人： Akira Iwasaki , Yukio Yamada , Yasuhiro Ikenaka , Yoshihiko Yasohara , Junzo Hasegawa
IPC分类号： C12P1304
CPC分类号： C12N9/1096 , C12P41/006
摘要： Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-&agr;-phenethylamine:pyruvate transaminase, which acts on (S)-&agr;-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-&agr;-phenethylamine:pyruvate transaminase is cultured.
摘要翻译：关于能够通过转氨酶有效地将酮化合物转化为光学活性氨基化合物的酶，以及使用该酶制备光学活性氨基化合物的方法。（S）-α-苯乙胺：丙酮酸转氨酶，其作用于（S）-α-苯乙胺和酮化合物，从而催化转氨酶形成苯乙酮和对应于酮化合物的氨基化合物; 使用转氨酶制备光学活性氨基化合物的方法; 以及培养用于制备上述转氨酶的微生物的方法，包括向培养基中加入一种或多种选自丙胺，1-丁胺，2-丁胺，2-戊胺，异丙胺和异丁胺的化合物作为诱导剂用于生产（S）-α-苯乙胺：丙酮酸转氨酶的微生物培养时的酶。

2. 发明授权

US07169592B2 Producing optically active amino compounds 有权
标题翻译：生产光学活性氨基化合物
公开(公告)号：US07169592B2
公开(公告)日：2007-01-30
申请号：US10112706
申请日：2002-04-02
申请人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Keiji Matsumoto , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
发明人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Keiji Matsumoto , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
IPC分类号： C12N9/10 , C12N1/21 , C12N15/54 , C12N15/63 , C12N15/82 , C12N15/85 , C07H21/04
CPC分类号： C12P13/001 , C12N9/1096
摘要： To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.
摘要翻译：提供通过微生物酶有效且廉价地制备主要含有（R） - 氨基化合物的光学活性化合物的方法; 具有立体选择性转氨酶活性的多肽，可适用于上述制备方法; 和编码该多肽的DNA。一种制备光学活性氨基化合物的方法，其特征在于通过在氨基受体上起转氨酶的作用，在氨基供体存在下的酮化合物，伯胺，立体选择性转氨酶; 包含编码具有立体选择性转氨酶活性的多肽的核苷酸序列的DNA; 和具有立体选择性转氨酶活性的多肽，其可从属于节杆菌属的微生物的培养物获得。

3. 发明授权

US06344351B2 (R)-transaminase from arthrobacter 失效
标题翻译：（R） - 转氨酶
公开(公告)号：US06344351B2
公开(公告)日：2002-02-05
申请号：US09794359
申请日：2001-02-28
申请人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
发明人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
IPC分类号： C12N910
CPC分类号： C12P13/001 , C12N9/1096 , C12P13/005 , C12P13/008 , C12P13/04 , C12P41/006 , Y10S435/83
摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.
摘要翻译：制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法，和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

4. 发明授权

US06221638B1 Process for producing optically active amino compounds 失效
标题翻译：光学活性氨基化合物的制造方法
公开(公告)号：US06221638B1
公开(公告)日：2001-04-24
申请号：US09064750
申请日：1998-04-23
申请人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
发明人： Yukio Yamada , Akira Iwasaki , Noriyuki Kizaki , Yasuhiro Ikenaka , Masahiro Ogura , Junzo Hasegawa
IPC分类号： C12P1300
CPC分类号： C12P13/001 , C12N9/1096 , C12P13/005 , C12P13/008 , C12P13/04 , C12P41/006 , Y10S435/83
摘要： The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.
摘要翻译：制备光学活性（R） - 氨基化合物的方法，其特征在于在酮化合物（氨基受体）的共存下通过（R） - 形式 - 特异性转氨酶的作用立体选择性地进行氨基转移的方法，和外消旋形式的氨基化合物（氨基供体）或（R） - 形式，得到光学活性（R） - 氨基化合物。根据本发明，可以容易地容易地制备其1-位上具有芳基等的光学活性（R） - 氨基化合物等，其通常难以制备。

5. 发明授权

US06762037B1 Process for producing coenzyme Q10 有权
标题翻译：生产辅酶Q10的方法
公开(公告)号：US06762037B1
公开(公告)日：2004-07-13
申请号：US09830111
申请日：2001-07-23
申请人： Hideyuki Matsuda , Makoto Kawamukai , Kazuyoshi Yajima , Yasuhiro Ikenaka , Junzo Hasegawa , Satomi Takahashi
发明人： Hideyuki Matsuda , Makoto Kawamukai , Kazuyoshi Yajima , Yasuhiro Ikenaka , Junzo Hasegawa , Satomi Takahashi
IPC分类号： C12P2102
CPC分类号： C12N9/1085 , C12P7/66
摘要： The present invention has for its object to isolate a gene coding for the enzyme synthesizing the coenzyme Q10 side chain from a fungal strain of the genus Saitoella and exploit it to advantage for the efficient microbial production of coenzyme Q10. The present invention provides; a DNA having the nucleotide sequence shown under SEQ ID NO:1 a DNA having a nucleotide sequence derived from the nucleotide sequence of SEQ ID NO:1 by the deletion, addition, insertion and/or substitution of one or a plurality of nucleotides and coding for a protein having decaprenyl diphosphate synthase activity a DNA which hybridizes with the DNA having the nucleotide sequence of SEQ ID NO:1 under a stringent condition and codes for a protein having decaprenyl diphosphate synthase activity.
摘要翻译：本发明的目的是从编码斋藤本的真菌菌株中分离编码合成辅酶Q10侧链的酶的基因，并有利于辅酶Q10的有效微生物产生。本发明提供了一种DNA 具有SEQ ID NO：1a所示的核苷酸序列，其具有通过删除，添加，插入和/或取代一个或多个核苷酸而编码具有SEQ ID NO：1的核苷酸序列的核苷酸序列的DNA，并编码具有SEQ ID NO：癸酰二磷酸合酶活性a在严格条件下与具有SEQ ID NO：1的核苷酸序列的DNA杂交的DNA，并编码具有十聚丙二酰二磷酸合成酶活性的蛋白质的DNA。

6. 发明授权

US06461842B1 Process for producing coenzyme Q10 失效
标题翻译：生产辅酶Q10的方法
公开(公告)号：US06461842B1
公开(公告)日：2002-10-08
申请号：US09673018
申请日：2000-10-10
申请人： Hideyuki Matsuda , Makoto Kawamukai , Kazuyoshi Yajima , Yasuhiro Ikenaka , Kenichi Nishi , Junzo Hasegawa , Satomi Takahashi
发明人： Hideyuki Matsuda , Makoto Kawamukai , Kazuyoshi Yajima , Yasuhiro Ikenaka , Kenichi Nishi , Junzo Hasegawa , Satomi Takahashi
IPC分类号： C12N910
CPC分类号： C12N9/1085 , C12P7/66
摘要： A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.
摘要翻译：从隶属于根瘤菌科的细菌中分离出参与辅酶Q10生物合成的关键基因十倍丙基二磷酸合酶基因。通过将该基因转移到微生物如大肠杆菌中并在其中表达，可以有效地产生辅酶Q10。

7. 发明授权

US5824522A Recombinant decarbamylases for producing D-.alpha.-amino acids 失效
标题翻译：用于产生D-α-氨基酸的重组脱氨基甲酰酶
公开(公告)号：US5824522A
公开(公告)日：1998-10-20
申请号：US294871
申请日：1994-08-22
申请人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi , Kazuma Okubo , Kazuhiko Yamada , Yoshiro Hiraishi
发明人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi , Kazuma Okubo , Kazuhiko Yamada , Yoshiro Hiraishi
IPC分类号： C12N9/80 , C12N9/96 , C12N11/02 , C12N11/08 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22 , C12P41/00 , C12N1/20 , C12N9/14 , C12N11/00
CPC分类号： C12N11/02 , C12N11/08 , C12N9/80 , C12N9/96 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22 , C12P41/009 , Y10S435/849 , Y10S435/874
摘要： Decarbamylases are provided capable of producing D-.alpha.-amino acids by hydrolysis of N-carbamyl-D-.alpha.-amino acids. A source of the decarbamylases is recombinant microorganisms produced by gene manipulation methods. Decarbamylases having improved thermostability can be obtained in which amino acids at a thermostability-related site of a natural decarbamylase have been replaced with other amino acids by mutating a DNA fragment encoding the natural decarbamylase. Recombinant DNA is obtained from a vector DNA and a DNA fragment encoding a natural decarbamylase where the nucleic acid sequence encoding an amino acid at a thermostability-related site is replaced with a nucleic acid sequence encoding another amino acid. The recombinant DNA is used to produce transformants that produce thermostable decarbamylases. The decarbamylases may be immobilized in purified, partially-purified or crude form or in the form of decarbamylase-containing microbial cells on a support such as a polymer having ion exchange groups or covalent bonding groups. The presence of an antioxidant such as dithiothreitol results in an immobilized decarbamylase preparation that can be repeatedly used 30 times or more.
摘要翻译：提供的脱甲酰氨基酶能够通过N-氨基甲酰基-D-α-氨基酸的水解产生D-氨基酸。脱氨基甲酰酶的来源是通过基因操作方法产生的重组微生物。通过突变编码天然脱氨基甲酰酶的DNA片段，可以得到具有改善的热稳定性的脱甲酰氨基磷酸酶，其中天然脱氨基甲酰酶的热稳定性相关部位的氨基酸已被其它氨基酸替代。从载体DNA和编码天然脱氨基甲酰酶的DNA片段获得重组DNA，其中将编码热稳定性相关部位的氨基酸的核酸序列替换为编码另一氨基酸的核酸序列。重组DNA用于产生产生热稳定性脱氨基甲酰酶的转化体。脱氨基甲酰酶可以固定在纯化的，部分纯化的或粗制的形式中，或以含脱氨基甲酰酶的微生物细胞的形式固定在载体上，例如具有离子交换基团或共价键合基团的聚合物。抗氧化剂如二硫苏糖醇的存在导致可重复使用30次以上的固定化脱氨基甲酰酶制剂。

8. 发明授权

US5695968A Process for production of D-.alpha.-amino acids 失效
标题翻译：生产D-α-氨基酸的方法
公开(公告)号：US5695968A
公开(公告)日：1997-12-09
申请号：US479638
申请日：1995-06-07
申请人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
发明人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
IPC分类号： C12N1/21 , C12N9/80 , C12N15/55 , C12P13/04 , C12P41/00 , C07H19/00 , C12N1/20 , C12P21/06
CPC分类号： C12P41/009 , C12N9/80 , C12P13/04
摘要： The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme. The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.
摘要翻译：本发明涉及与D-N-氨基甲酰基-α-氨基酸酰胺水解酶相关的基因，其是能够将D-N-氨基甲酰基-α-氨基酸转化为D-α-氨基酸的酶; 将含有该基因的DNA片段并入载体的重组质粒; 通过将重组质粒并入而转化的属于埃希氏杆菌属，假单胞菌属，黄杆菌属，芽孢杆菌属，沙雷氏菌属，棒状杆菌属或短杆菌属的微生物; 制备D-N-氨基甲酰基-α-氨基酸酰胺水解酶的方法，包括培养转化的微生物并从其中收集所需产物的步骤; 通过该方法获得的D-N-氨基甲酰基-α-氨基酸酰胺水解酶; 以及借助于酶的作用生产D-氨基酸的方法。 D-N-氨基甲酰基-α-氨基酸酰胺水解酶可以固定在用于固定的载体上并用作固定化酶。

9. 发明授权

US5902736A Process for the production of D-.alpha.-amino acids by hydrolysis of the corresponding N-carbamyl derivative 失效
标题翻译：通过水解相应的N-氨基甲酰基衍生物生产D-氨基酸的方法
公开(公告)号：US5902736A
公开(公告)日：1999-05-11
申请号：US244657
申请日：1994-06-06
申请人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
发明人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
IPC分类号： C12P13/04 , C12P41/00 , C07C1/04
CPC分类号： C12P13/04 , C12P41/009 , Y10S435/874 , Y10S435/911
摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.
摘要翻译： PCT No.PCT / JP93 / 01408 Sec。 371日期：1994年6月6日 102（e）日期1994年6月6日PCT提交1993年10月1日PCT公布。公开号WO94 / 08030 日期1994年4月14日在制备D-氨基酸的方法中，其中N-氨基甲酰基-D-α-氨基酸对应于通式：其中R表示苯基，羟基取代的苯基，取代的或未取代的烷基或噻吩基通过微生物酶在水性介质中转化成对应于以下通式的D-α-氨基酸：其中R与上述定义相同，由Comamonas属的微生物产生的脱氨基甲酰酶，使用喷杆菌属，产碱杆菌属，孢子体菌属，根瘤菌属，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。

10. 发明授权

US5962279A Process for producing D-amino acids with composite immobilized enzyme preparation 失效
标题翻译：用复合固定化酶制剂生产D-氨基酸的方法
公开(公告)号：US5962279A
公开(公告)日：1999-10-05
申请号：US596144
申请日：1996-08-16
申请人： Hirokazu Nanba , Yukio Yamada , Kazuyoshi Yajima , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Takehisa Ohashi
发明人： Hirokazu Nanba , Yukio Yamada , Kazuyoshi Yajima , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Takehisa Ohashi
IPC分类号： C12P13/04 , C12P41/00 , C12N11/00
CPC分类号： C12P13/04 , C12P41/009
摘要： A process for the efficient production of a D-amino acid from the corresponding DL-5-substituted hydantoin by one-step reaction which comprises using a composite immobilized enzyme at a pH about neutrality, said composite immobilized enzyme being obtained by immobilizing a hydantoinase having its optimal pH within an alkaline range and a D-N-carbamyl-.alpha.-amino acid amidohydrolase having its optimal pH about neutrality in a coexisting state on an immobilizing support, simultaneously, is disclosed.
摘要翻译： PCT No.PCT / JP95 / 01257第 371日期：1996年8月16日 102（e）日期1996年8月16日PCT提交1995年6月23日PCT公布。公开号WO96 / 00296 日期1996年1月4日一种通过一步反应从相应的DL-5-取代的乙内酰脲有效生产D-氨基酸的方法，其包括在pH约为中性的情况下使用复合固定化酶，所述复合固定化酶得到通过将其最佳pH在碱性范围内的乙内酰脲固定化，并且同时具有在共存状态下具有最佳pH约中性的DN-氨基甲酰-α-氨基酸酰胺水解酶。

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