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1. 发明授权

US5962279A Process for producing D-amino acids with composite immobilized enzyme preparation 失效
标题翻译：用复合固定化酶制剂生产D-氨基酸的方法
公开(公告)号：US5962279A
公开(公告)日：1999-10-05
申请号：US596144
申请日：1996-08-16
申请人： Hirokazu Nanba , Yukio Yamada , Kazuyoshi Yajima , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Takehisa Ohashi
发明人： Hirokazu Nanba , Yukio Yamada , Kazuyoshi Yajima , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Takehisa Ohashi
IPC分类号： C12P13/04 , C12P41/00 , C12N11/00
CPC分类号： C12P13/04 , C12P41/009
摘要： A process for the efficient production of a D-amino acid from the corresponding DL-5-substituted hydantoin by one-step reaction which comprises using a composite immobilized enzyme at a pH about neutrality, said composite immobilized enzyme being obtained by immobilizing a hydantoinase having its optimal pH within an alkaline range and a D-N-carbamyl-.alpha.-amino acid amidohydrolase having its optimal pH about neutrality in a coexisting state on an immobilizing support, simultaneously, is disclosed.
摘要翻译： PCT No.PCT / JP95 / 01257第 371日期：1996年8月16日 102（e）日期1996年8月16日PCT提交1995年6月23日PCT公布。公开号WO96 / 00296 日期1996年1月4日一种通过一步反应从相应的DL-5-取代的乙内酰脲有效生产D-氨基酸的方法，其包括在pH约为中性的情况下使用复合固定化酶，所述复合固定化酶得到通过将其最佳pH在碱性范围内的乙内酰脲固定化，并且同时具有在共存状态下具有最佳pH约中性的DN-氨基甲酰-α-氨基酸酰胺水解酶。

2. 发明授权

US5824522A Recombinant decarbamylases for producing D-.alpha.-amino acids 失效
标题翻译：用于产生D-α-氨基酸的重组脱氨基甲酰酶
公开(公告)号：US5824522A
公开(公告)日：1998-10-20
申请号：US294871
申请日：1994-08-22
申请人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi , Kazuma Okubo , Kazuhiko Yamada , Yoshiro Hiraishi
发明人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi , Kazuma Okubo , Kazuhiko Yamada , Yoshiro Hiraishi
IPC分类号： C12N9/80 , C12N9/96 , C12N11/02 , C12N11/08 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22 , C12P41/00 , C12N1/20 , C12N9/14 , C12N11/00
CPC分类号： C12N11/02 , C12N11/08 , C12N9/80 , C12N9/96 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/22 , C12P41/009 , Y10S435/849 , Y10S435/874
摘要： Decarbamylases are provided capable of producing D-.alpha.-amino acids by hydrolysis of N-carbamyl-D-.alpha.-amino acids. A source of the decarbamylases is recombinant microorganisms produced by gene manipulation methods. Decarbamylases having improved thermostability can be obtained in which amino acids at a thermostability-related site of a natural decarbamylase have been replaced with other amino acids by mutating a DNA fragment encoding the natural decarbamylase. Recombinant DNA is obtained from a vector DNA and a DNA fragment encoding a natural decarbamylase where the nucleic acid sequence encoding an amino acid at a thermostability-related site is replaced with a nucleic acid sequence encoding another amino acid. The recombinant DNA is used to produce transformants that produce thermostable decarbamylases. The decarbamylases may be immobilized in purified, partially-purified or crude form or in the form of decarbamylase-containing microbial cells on a support such as a polymer having ion exchange groups or covalent bonding groups. The presence of an antioxidant such as dithiothreitol results in an immobilized decarbamylase preparation that can be repeatedly used 30 times or more.
摘要翻译：提供的脱甲酰氨基酶能够通过N-氨基甲酰基-D-α-氨基酸的水解产生D-氨基酸。脱氨基甲酰酶的来源是通过基因操作方法产生的重组微生物。通过突变编码天然脱氨基甲酰酶的DNA片段，可以得到具有改善的热稳定性的脱甲酰氨基磷酸酶，其中天然脱氨基甲酰酶的热稳定性相关部位的氨基酸已被其它氨基酸替代。从载体DNA和编码天然脱氨基甲酰酶的DNA片段获得重组DNA，其中将编码热稳定性相关部位的氨基酸的核酸序列替换为编码另一氨基酸的核酸序列。重组DNA用于产生产生热稳定性脱氨基甲酰酶的转化体。脱氨基甲酰酶可以固定在纯化的，部分纯化的或粗制的形式中，或以含脱氨基甲酰酶的微生物细胞的形式固定在载体上，例如具有离子交换基团或共价键合基团的聚合物。抗氧化剂如二硫苏糖醇的存在导致可重复使用30次以上的固定化脱氨基甲酰酶制剂。

3. 发明授权

US5695968A Process for production of D-.alpha.-amino acids 失效
标题翻译：生产D-α-氨基酸的方法
公开(公告)号：US5695968A
公开(公告)日：1997-12-09
申请号：US479638
申请日：1995-06-07
申请人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
发明人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
IPC分类号： C12N1/21 , C12N9/80 , C12N15/55 , C12P13/04 , C12P41/00 , C07H19/00 , C12N1/20 , C12P21/06
CPC分类号： C12P41/009 , C12N9/80 , C12P13/04
摘要： The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme. The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.
摘要翻译：本发明涉及与D-N-氨基甲酰基-α-氨基酸酰胺水解酶相关的基因，其是能够将D-N-氨基甲酰基-α-氨基酸转化为D-α-氨基酸的酶; 将含有该基因的DNA片段并入载体的重组质粒; 通过将重组质粒并入而转化的属于埃希氏杆菌属，假单胞菌属，黄杆菌属，芽孢杆菌属，沙雷氏菌属，棒状杆菌属或短杆菌属的微生物; 制备D-N-氨基甲酰基-α-氨基酸酰胺水解酶的方法，包括培养转化的微生物并从其中收集所需产物的步骤; 通过该方法获得的D-N-氨基甲酰基-α-氨基酸酰胺水解酶; 以及借助于酶的作用生产D-氨基酸的方法。 D-N-氨基甲酰基-α-氨基酸酰胺水解酶可以固定在用于固定的载体上并用作固定化酶。

4. 发明授权

US5902736A Process for the production of D-.alpha.-amino acids by hydrolysis of the corresponding N-carbamyl derivative 失效
标题翻译：通过水解相应的N-氨基甲酰基衍生物生产D-氨基酸的方法
公开(公告)号：US5902736A
公开(公告)日：1999-05-11
申请号：US244657
申请日：1994-06-06
申请人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
发明人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
IPC分类号： C12P13/04 , C12P41/00 , C07C1/04
CPC分类号： C12P13/04 , C12P41/009 , Y10S435/874 , Y10S435/911
摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.
摘要翻译： PCT No.PCT / JP93 / 01408 Sec。 371日期：1994年6月6日 102（e）日期1994年6月6日PCT提交1993年10月1日PCT公布。公开号WO94 / 08030 日期1994年4月14日在制备D-氨基酸的方法中，其中N-氨基甲酰基-D-α-氨基酸对应于通式：其中R表示苯基，羟基取代的苯基，取代的或未取代的烷基或噻吩基通过微生物酶在水性介质中转化成对应于以下通式的D-α-氨基酸：其中R与上述定义相同，由Comamonas属的微生物产生的脱氨基甲酰酶，使用喷杆菌属，产碱杆菌属，孢子体菌属，根瘤菌属，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。

5. 发明授权

US5863785A Decarbamylase isolated from comamonas or blastobacter 失效
标题翻译：从comamonas或blastobacter分离的脱氨基甲酰酶
公开(公告)号：US5863785A
公开(公告)日：1999-01-26
申请号：US479639
申请日：1995-06-07
申请人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
发明人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
IPC分类号： C12P13/04 , C12P41/00 , C12N9/78 , C12N9/80
CPC分类号： C12P13/04 , C12P41/009 , Y10S435/874 , Y10S435/911
摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.
摘要翻译：在制备D-α-氨基酸的方法中，其中相应于通式为：苯基，取代或未取代的烷基或噻吩基的N-氨基甲酰基-D-α-氨基酸被转化通过微生物酶在水性介质中与对应于以下通式的D-α-氨基酸反应：其中R与上述定义相同，由Comamonas属，Blastobacter，Alcaligenes，Sporosarcina属的微生物产生的脱氨基甲酰酶，使用根瘤菌，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-α-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。

6. 发明授权

US6083752A DNA coding for decarbamylase improved in thermostability and use thereof 失效
标题翻译：编码脱氨基甲酰酶的DNA改善了热稳定性和使用
公开(公告)号：US6083752A
公开(公告)日：2000-07-04
申请号：US876398
申请日：1997-06-16
申请人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi
发明人： Yasuhiro Ikenaka , Hirokazu Nanba , Masayuki Takano , Kazuyoshi Yajima , Yukio Yamada , Satomi Takahashi
IPC分类号： C12N9/80 , C12N15/55 , C12N15/72 , C12P13/06 , C12P13/08 , C12P13/22 , C12N15/00 , C12N5/10 , C12N9/14
CPC分类号： C12N15/72 , C12N9/80 , C12P13/06 , C12P13/08 , C12P13/22
摘要： A DNA fragment coding for a decarbamylase protein improved in thermostability as the result of replacement of at least one base of a DNA fragment coding for a decarbamylase protein derived from a microorganism with another base and the resultant replacement of at least one of the corresponding amino acids, and its production process; a vector containing the DNA fragment; a transformant obtained by transformation with the vector; as well as a decarbamylase improved in thermostability and its production process. Also disclosed is a process for producing a D-.alpha.-amino acid, which comprises converting an N-carbamoyl-D-.alpha.-amino acid into the corresponding D-.alpha.-amino acid in an aqueous medium by the action of a decarbamylase having a thermostable temperature of 65.degree. C. or higher; and collecting the D-.alpha.-amino acid produced.
摘要翻译：由于将来自微生物的脱氨基甲酰酶蛋白质的至少一个碱基替换为另一碱基的氨基酸残基的至少一个碱基，所以替代了至少一个相应的氨基酸，及其生产工艺; 含有DNA片段的载体; 通过用载体转化获得的转化体; 以及改进了热稳定性的脱氨基甲酰酶及其制备方法。还公开了一种制备D-α-氨基酸的方法，该方法包括通过脱氨基甲酰酶的作用将N-氨基甲酰基-D-α-氨基酸转化成水性介质中的相应的D- 耐热温度在65℃以上; 并收集产生的D-α-氨基酸。

7. 发明授权

US5565344A Process for production of D-.alpha.-amino acids 失效
标题翻译：生产D-α-氨基酸的方法
公开(公告)号：US5565344A
公开(公告)日：1996-10-15
申请号：US917111
申请日：1992-08-07
申请人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
发明人： Hirokazu Nanba , Yukio Yamada , Masayuki Takano , Yasuhiro Ikenaka , Satomi Takahashi , Kazuyoshi Yajima
IPC分类号： C12N1/21 , C12N9/80 , C12N15/55 , C12P13/04 , C12P41/00 , C07H19/00 , C12N1/12 , C12N9/14
CPC分类号： C12P41/009 , C12N9/80 , C12P13/04
摘要： The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme.The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.
摘要翻译： PCT No.PCT / JP91 / 01696 Sec。 371日期：1992年8月7日 102（e）日期1992年8月7日PCT 1991年12月6日PCT PCT。出版物WO92 / 10579 日期1992年6月25日本发明涉及与能够将D-N-氨基甲酰基-α-氨基酸转化为D-氨基酸的酶的D-N-氨基甲酰基-α-氨基酸酰胺水解酶相关的基因。将含有该基因的DNA片段并入载体的重组质粒; 通过将重组质粒并入而转化的属于埃希氏杆菌属，假单胞菌属，黄杆菌属，芽孢杆菌属，沙雷氏菌属，棒状杆菌属或短杆菌属的微生物; 制备D-N-氨基甲酰基-α-氨基酸酰胺水解酶的方法，包括培养转化的微生物并从其中收集所需产物的步骤; 通过该方法获得的D-N-氨基甲酰基-α-氨基酸酰胺水解酶; 以及借助于酶的作用生产D-氨基酸的方法。 D-N-氨基甲酰基-α-氨基酸酰胺水解酶可以固定在用于固定的载体上并用作固定化酶。

8. 发明申请

US20110086396A1 D-AMINO ACID OXIDASE, AND METHOD FOR PRODUCTION OF L-AMINO ACID, 2-OXO ACID, OR CYCLIC IMINE 有权
标题翻译： D-氨基酸氧化物及其制备L-氨基酸，2-氧代酸或环状体的方法
公开(公告)号：US20110086396A1
公开(公告)日：2011-04-14
申请号：US11997861
申请日：2006-08-02
申请人： Hiroyuki Kanamaru , Makoto Ueda , Ruuji Miki , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Ruuji Miki , Hirokazu Nanba
IPC分类号： C12P13/04 , C12N15/53 , C12N9/02 , C12N15/63 , C12N1/00
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10
摘要： The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
摘要翻译：本发明涉及从念珠菌中间体分离纯化的新型D-氨基酸氧化酶，编码D-氨基酸氧化酶的基因，含有该基因的重组质粒，以及引入了D-氨基酸氧化酶基因的转化体以及D-氨基酸氧化酶的制造方法，包括培养转化体。此外，本发明涉及L-氨基酸，2-氧酸或环状亚胺的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶反应，更优选L-氨基酸的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶，氨基酸脱氢酶和具有辅酶再生活性的酶反应。根据本发明，可以以工业规模高效率地制备L-氨基酸，2-氧代酸或环状亚胺。

9. 发明授权

US07655450B2 Polypeptide having amidase activity and gene thereof 有权
标题翻译：具有酰胺酶活性的多肽及其基因
公开(公告)号：US07655450B2
公开(公告)日：2010-02-02
申请号：US10587085
申请日：2005-01-26
申请人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
发明人： Satohiro Yanagisawa , Makoto Ueda , Hirokazu Nanba
IPC分类号： C12N9/78 , C07H21/04 , C12N15/63 , C12N1/20 , C12P21/00
CPC分类号： C12R1/06 , C12N9/80 , C12P13/04 , C12P13/22 , C12P41/006 , C12Y305/01004
摘要： It is an object of the present invention to provide a novel amidase that is useful for production of an optically active amino acid, and in particular, a D-amino acid, and a production method thereof.The present invention relates to a novel D-amidase isolated and purified from the Arthrobacter sp. KNK1101J, a gene encoding the above amidase, a recombinant plasmid comprising the above gene, and a transformant into which the above amidase gene has been introduced. In addition, the present invention also relates to a method for producing the amidase, comprising culturing the Arthrobacter sp. KNK1101J or the above transformant, and collecting the above amidase.
摘要翻译：本发明的目的是提供一种可用于制备光学活性氨基酸，特别是D-氨基酸的新型酰胺酶及其制备方法。本发明涉及从节杆菌属分离和纯化的新型D-酰胺酶。 KNK1101J，编码上述酰胺酶的基因，包含上述基因的重组质粒，以及引入了上述酰胺酶基因的转化体。此外，本发明还涉及一种生产酰胺酶的方法，包括培养节杆菌属（Arthrobacter sp。 KNK1101J或上述转化体，并收集上述酰胺酶。

10. 发明授权

US08227228B2 D-amino acid oxidase, and method for production of L-amino acid, 2-oxo acid, or cyclic imine 有权
标题翻译： D-氨基酸氧化酶，以及L-氨基酸，2-氧代酸或环状亚胺的制备方法
公开(公告)号：US08227228B2
公开(公告)日：2012-07-24
申请号：US11997861
申请日：2006-08-02
申请人： Hiroyuki Kanamaru , Makoto Ueda , Ryuuji Miki , Hirokazu Nanba
发明人： Hiroyuki Kanamaru , Makoto Ueda , Ryuuji Miki , Hirokazu Nanba
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12P21/06 , C12P19/34 , C12P13/04 , C07H21/04 , C07K1/00
CPC分类号： C12P7/40 , C12N9/0024 , C12P13/04 , C12P17/10
摘要： The present invention relates to novel D-amino acid oxidase isolated and purified from Candida intermedia, a gene encoding the D-amino acid oxidase, a recombinant plasmid containing the gene, and a transformant into which the D-amino acid oxidase gene has been introduced, as well as a production method of D-amino acid oxidase including culturing the transformant. Moreover, the present invention relates to a production method of L-amino acids, 2-oxo acids or cyclic imines, which include reacting racemic amino acids with the D-amino acid oxidase, more preferably, a production method of L-amino acids, which includes reacting racemic amino acid with the D-amino acid oxidase, amino acid dehydrogenase and an enzyme having a coenzyme-regenerating activity. According to the present invention, L-amino acids, 2-oxo acids or cyclic imines can be produced with good efficiency in an industrial scale.
摘要翻译：本发明涉及从念珠菌中间体分离纯化的新型D-氨基酸氧化酶，编码D-氨基酸氧化酶的基因，含有该基因的重组质粒，以及引入了D-氨基酸氧化酶基因的转化体以及D-氨基酸氧化酶的制造方法，包括培养转化体。此外，本发明涉及L-氨基酸，2-氧酸或环状亚胺的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶反应，更优选L-氨基酸的制造方法，其包括使外消旋氨基酸与D-氨基酸氧化酶，氨基酸脱氢酶和具有辅酶再生活性的酶反应。根据本发明，可以以工业规模高效率地制备L-氨基酸，2-氧代酸或环状亚胺。

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