专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20050170481A1 Novel (d)-2-hydroxy-acid oxidase derived from microorganism and biochemical process for producing glyoxylic acid therewith 审中-公开
标题翻译：来自微生物的新型（d）-2-羟基酸氧化酶和与其生产乙醛酸的生化方法
公开(公告)号：US20050170481A1
公开(公告)日：2005-08-04
申请号：US10513432
申请日：2003-05-22
申请人： Akira Iwasaki , Takehiko Matsumoto , Motohisa Washida , Hiroshi Watanabe , Sakayu Shimizu
发明人： Akira Iwasaki , Takehiko Matsumoto , Motohisa Washida , Hiroshi Watanabe , Sakayu Shimizu
IPC分类号： C12N9/04 , C12P7/58 , C12P7/50 , C12N1/21 , C12N9/02
CPC分类号： C12N9/0006 , C12P7/58
摘要： The present invention provides an industrially advantageous process for producing glyoxylic acid by a biochemical procedure using oxidase. In particular, the present invention provides novel (D)-2-hydroxy-acid oxidase acting on a 2-hycoxy acid to produce a corresponding 2-keto acid, and a process for producing glyoxylic acid in which the 2-hydroxy-acid oxidase, a culture solution of microorganisms capable of producing the enzyme, microorganism cells isolated from the culture solution, or a product of treatment of microorganism cells acts on glycolic acid to convert the glycolic acid to glyoxylic acid.
摘要翻译：本发明提供了通过使用氧化酶的生物化学方法生产乙醛酸的工业上有利的方法。特别地，本发明提供作用于2-羟基酸以产生相应的2-酮酸的新的（D）-2-羟基酸氧化酶，以及生产二羟基酸的方法，其中2-羟基酸氧化酶，能够生产酶的微生物培养液，从培养液中分离的微生物细胞或微生物细胞处理的产物作用于乙醇酸以将乙醇酸转化成乙醛酸。

2. 发明申请

US20070026510A1 Process for biochemical production of glyoxylic acid 审中-公开
标题翻译：乙醛酸生化生产工艺
公开(公告)号：US20070026510A1
公开(公告)日：2007-02-01
申请号：US10544618
申请日：2004-02-13
申请人： Akira Iwasaki , Takehiro Matsumoto , Motohisa Washida , Hiroshi Watanabe , Junzou Hasegawa , Sakayu Shimizu
发明人： Akira Iwasaki , Takehiro Matsumoto , Motohisa Washida , Hiroshi Watanabe , Junzou Hasegawa , Sakayu Shimizu
IPC分类号： C12P7/50 , C12N1/20
CPC分类号： C12P7/40
摘要： The present invention provides an industrially advantageous process for biochemical production of glyoxylic acid from glyoxal. More specifically, the present invention provides a process for production of glyoxylic acid, which is characterized in that the process comprises allowing oxidoreductase that can convert glyoxal into glyoxylic acid, such as oxidase and dehydrogenase, to act on glyoxal, so as to convert glyoxal into glyoxylic acid.
摘要翻译：本发明提供了从乙二醛生物化生产乙醛酸的工业上有利的方法。更具体地说，本发明提供了一种生产乙醛酸的方法，其特征在于该方法包括允许将乙二醛转化为乙醛酸（如氧化酶和脱氢酶）的氧化还原酶作用于乙二醛，从而将乙二醛转化为乙醛酸。

3. 发明授权

US08273562B2 Method for producing 4-hydroxy-L-isoleucine 有权
公开(公告)号：US08273562B2
公开(公告)日：2012-09-25
申请号：US13344110
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov, legal representative , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12Q1/68 , C12P21/06 , C12P21/04 , C07H21/04
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

4. 发明授权

US07927843B2 Method of preparing 2-deoxyribose 5-phosphate 有权
标题翻译： 2-脱氧核糖5-磷酸的制备方法
公开(公告)号：US07927843B2
公开(公告)日：2011-04-19
申请号：US11878558
申请日：2007-07-25
申请人： Sakayu Shimizu , Jun Ogawa
发明人： Sakayu Shimizu , Jun Ogawa
IPC分类号： C12P19/02
CPC分类号： C12R1/22 , C12N9/88 , C12N9/90 , C12P19/02
摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.
摘要翻译：本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸盐的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法，微生物。本发明还公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸盐的微生物本身存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者源于微生物的酶。

5. 发明申请

US20080261281A1 Novel Carbonyl Reductase, Gene Thereof and Method of Using the Same 有权
标题翻译：新型羰基还原酶，其基因及其使用方法
公开(公告)号：US20080261281A1
公开(公告)日：2008-10-23
申请号：US11659481
申请日：2005-08-01
申请人： Noriyuki Kizaki , Miho Yano , Masahiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
发明人： Noriyuki Kizaki , Miho Yano , Masahiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12N9/14 , C12N15/52 , C12N1/21 , C12P13/02
CPC分类号： C12P13/02 , C12N9/0006 , C12P41/002
摘要： The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant.Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
摘要翻译：本发明涉及具有不对称还原（3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁酮的活性以产生（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4- 从属于Ogataea的微生物分离的丁氧基羰基氨基-4-苯基-2-丁醇，编码多肽的DNA和产生多肽的转化体。本发明还涉及利用多肽或转化体制备（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇的方法。使用本发明的多肽或转化体，可以有效地制备光学活性醇如（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇等。

6. 发明申请

US20070298468A1 Method of preparing 2-deoxyribose 5-phosphate 有权
公开(公告)号：US20070298468A1
公开(公告)日：2007-12-27
申请号：US11878558
申请日：2007-07-25
申请人： Sakayu Shimizu , Jun Ogawa
发明人： Sakayu Shimizu , Jun Ogawa
IPC分类号： C12P19/02
CPC分类号： C12R1/22 , C12N9/88 , C12N9/90 , C12P19/02
摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.

7. 发明授权

US06974685B2 High production method of prenyl alcohol using saccharomyces cells 失效
标题翻译：使用酵母细胞制备异戊醇的高生产方法
公开(公告)号：US06974685B2
公开(公告)日：2005-12-13
申请号：US10022434
申请日：2001-12-20
申请人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
发明人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
IPC分类号： C12N1/38 , C12P7/04 , C12R1/645 , C12P7/02
CPC分类号： C12N1/38 , C12P7/04
摘要： The present invention provides a high production method of prenyl alcohol, which comprises culturing prenyl alcohol-producing cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene to produce and accumulate prenyl alcohol in the cells; allowing the accumulated prenyl alcohol to be secreted from the cells; and then collecting prenyl alcohol. The present invention enables prenyl alcohol to be highly produced in and effectively secreted from prenyl alcohol-producing cells by culturing the cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene.
摘要翻译：本发明提供了高产的异戊醇的制备方法，其包括在选自表面活性剂，脂肪或油中的至少一种的存在下，在具有增加的糖含量的培养基中培养异戊醇产生细胞，和萜烯在细胞中产生和积累异戊醇; 允许从细胞中分泌积累的异戊醇; 然后收集异戊醇。本发明通过在选自表面活性剂，表面活性剂，表面活性剂的至少一种的存在下，在具有增加的糖含量的介质中培养细胞，使异戊烯醇高产生并由异戊烯醇产生细胞有效分泌脂肪或油，和萜烯。

8. 发明授权

US06972191B2 Method for production of geranylgeraniol and analogous compounds thereof by microorganisms 失效
标题翻译：通过微生物生产香叶基香叶醇及其类似物的方法
公开(公告)号：US06972191B2
公开(公告)日：2005-12-06
申请号：US10022695
申请日：2001-12-20
申请人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
发明人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
IPC分类号： C07C33/02 , C12P7/04 , C12R1/85 , C12P7/02
CPC分类号： C12P7/04
摘要： The present invention provides a method for producing geranylgeraniol and analogous compounds thereof, which comprises culturing yeast cells (ascomycetes and deuteromycetes), bacterial cells, actinomycete cells or filamentous fungus cells, all of which are capable of producing geranylgeraniol and analogous compounds thereof, in a medium to produce and accumulate geranylgeraniol and analogous compounds thereof in the cells and/or in the extracellular environment; and then collecting these compounds. The present invention enables inexpensive mass production of geranylgeraniol and analogous compounds thereof by using microorganisms capable of producing geranylgeraniol, farnesol and/or nerolidol useful as biosynthetic intermediates of terpenes, carotenoids and/or steroids.
摘要翻译：本发明提供了一种生产香叶基香叶醇及其类似化合物的方法，其包括培养酵母细胞（子囊菌和氘核菌素），细菌细胞，放线菌细胞或丝状真菌细胞，所有这些能够产生香叶基香叶醇及其类似物培养基以在细胞和/或细胞外环境中产生和积累香叶基香叶醇及其类似物; 然后收集这些化合物。本发明可以通过使用能够产生香叶基香叶醇，法呢醇和/或橙花叔醇作为萜烯，类胡萝卜素和/或类固醇的生物合成中间体的微生物，从而廉价地批量生产香叶基香叶醇及其类似物。

9. 发明授权

US06444460B1 Microbial production of actinol 有权
标题翻译：微生物生成的肌动蛋白
公开(公告)号：US06444460B1
公开(公告)日：2002-09-03
申请号：US09375129
申请日：1999-08-16
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12P726
CPC分类号： C12R1/06 , C12P7/26 , C12P41/002 , C12R1/01 , C12R1/15
摘要： A process for making (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone by contacting (6R)-2,2,6-trimethylcyclohexanedione with a microorganism which is selected from microorganisms of the genera Cellulomonas, Corynebacterium, Planococcus and Arthrobacter and which is capable of the selective asymmetric reduction of (6R)-2,2,6-trimethylcyclohexanedione to (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone, and recovering the resulting (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone from the reaction mixture. The selective asymmetric reduction can be effected in the presence of a co-factor, such as, nicotinamide adenine dinucleotide (NAD), nicotinamide adenine dinucleotide phosphate (NADP), or said co-factor with glucose and glucose dehydrogenase (GDH), and/or in the presence of a surfactant. The product is useful for the synthesis of carotenoids, such as, zeaxanthin.
摘要翻译：通过使（6R）-2,2,6-三甲基环己二酮与微生物接触，制备（4R，6R）-4-羟基-2,2,6-三甲基环己酮的方法，所述微生物选自Cellulomonas，Corynebacterium，Planococcus 并且能够（6R）-2,2,6-三甲基环己二酮选择性不对称还原成（4R，6R）-4-羟基-2,2,6-三甲基环己酮，并回收得到的（4R，6R ）-4-羟基-2,2,6-三甲基环己酮。选择性不对称还原可以在辅因子存在下进行，例如烟酰胺腺嘌呤二核苷酸（NAD），烟酰胺腺嘌呤二核苷酸磷酸（NADP）或与葡萄糖和葡萄糖脱氢酶（GDH）的所述辅因子，和/ 或在表面活性剂存在下。该产品可用于合成类胡萝卜素，如玉米黄质。

10. 发明授权

US06406898B1 D-pantolactone hydrolase and gene encoding the same 失效
标题翻译： D-泛酸内酯水解酶和编码基因的基因
公开(公告)号：US06406898B1
公开(公告)日：2002-06-18
申请号：US08836047
申请日：1997-09-19
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu , Michihiko Kobayashi
IPC分类号： C12N914
CPC分类号： C12N9/18 , C12P17/04 , C12P41/001 , C12P41/005
摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.
摘要翻译：提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式