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    • 2. 发明授权
    • Method for introducing a gene into labyrinthulomycota
    • 将基因导入迷路神经霉菌的方法
    • US07888123B2
    • 2011-02-15
    • US11413173
    • 2006-04-28
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • C12N15/74C12P21/06C12N5/02C07H21/04
    • C12N15/80C12N15/11
    • An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
    • 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统,以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了将转基因导入到迷路神经霉素细胞中的方法,其包括将包含转基因和核苷酸序列的重组载体导入到迷路形霉菌属细胞中,该载体与Labyrinthuloycycota的一部分染色体DNA同源且能够同源 与染色体DNA重组,然后在该同源核苷酸序列中诱导同源重组。
    • 5. 发明申请
    • Vector capable for transformation of labyrinthulomycota
    • 能够转化迷路神经霉菌的载体
    • US20060286650A1
    • 2006-12-21
    • US11413141
    • 2006-04-28
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • C12P7/64C12N15/74C12N1/21
    • C12P7/6463C12N15/79C12P7/6409
    • An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
    • 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统,以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了用转基因转化迷路孢霉菌纲的载体,其包含至少(1)与Labyrinthuloycycota的一部分染色体DNA同源且能够与染色体DNA同源重组的核苷酸序列,(2) 具有位于上游的启动子序列和位于下游的终止子序列的选择标记基因,和(3)位于上游的位于上游的启动子序列和位于下游的终止子序列的转基因插入的克隆位点。
    • 6. 发明申请
    • Method for introducing a gene into Labyrinthulomycota
    • 将基因导入迷迭香霉菌的方法
    • US20060275904A1
    • 2006-12-07
    • US11413173
    • 2006-04-28
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • Kazuhisa OnoTsunehiro AkiSeiji Kawamoto
    • C12N15/74C12N1/21
    • C12N15/80C12N15/11
    • An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
    • 本发明的一个目的是提供一种能够阐明脂质如PUFA和类胡萝卜素的生物合成机理的Labyrinthuloycycota的转化系统,以及构建高生产系统以及新型功能性脂质分子的设计和开发 机制的控制。 本发明提供了将转基因导入到迷路神经霉素细胞中的方法,其包括将包含转基因和核苷酸序列的重组载体导入到迷路形霉菌属细胞中,该载体与Labyrinthuloycycota的一部分染色体DNA同源且能够同源 与染色体DNA重组,然后在该同源核苷酸序列中诱导同源重组。
    • 7. 发明申请
    • Chain-driving mechanism and conveyor apparatus
    • 链条驱动机构和输送机
    • US20060225992A1
    • 2006-10-12
    • US10550065
    • 2004-03-08
    • Katsuyoshi TachibanaSeiji Kawamoto
    • Katsuyoshi TachibanaSeiji Kawamoto
    • B65G13/06
    • B65G13/07
    • The chain-driving mechanism and the conveyor apparatus of the present invention include a plurality of rollers (20) arranged to convey to-be-conveyed objects, a driving mechanism that drives the rollers (20) in conjunction with each other, and a motor (80) that exerts a driving force onto the driving mechanism. The driving mechanism includes a plurality of sprockets (40) that rotate coaxially with and together with the rollers (20), an endless chain (50) wound onto the sprockets (40), and a chain guide (60, 61, 62) disposed adjacent to the chain (50) so as to sandwich the chain (50) from the outside in the upward and downward directions. With this structure, there is no need to adjust the stretch of the chain, and the chain can be prevented from slipping off. Additionally, the structure can be made simpler, and costs can be made smaller.
    • 本发明的链条驱动机构和输送装置包括多个辊(20),其布置成输送被输送物体;驱动机构,其相互连动地驱动辊(20);以及马达 (80),其向驱动机构施加驱动力。 驱动机构包括与滚子(20)同轴并与其一起旋转的多个链轮(40),缠绕在链轮(40)上的环形链条(50),以及设置在链轮 邻近链条(50),以便沿向上和向下的方向从外部夹紧链条(50)。 利用这种结构,不需要调整链条的拉伸,并且可以防止链条滑落。 此外,可以使结构更简单,并且可以使成本更小。