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1. 发明申请

US20140323358A1 Vacuum Assist for a Microplate 审中-公开
标题翻译：真空辅助微孔板
公开(公告)号：US20140323358A1
公开(公告)日：2014-10-30
申请号：US14326243
申请日：2014-07-08
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Mark F. Oldham , Adrian Fawcett
IPC分类号： C12Q1/68
CPC分类号： B01L3/5085 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/545 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/14 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12Q1/686 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A vacuum assist apparatus can comprise a microplate. The microplate can comprise a first surface and an opposing second surface. A plurality of wells can be formed in the first surface of the microplate. Each of the plurality of wells can be sized to receive an assay therein. A support base can comprise a fluid passage. The microplate can be positioned adjacent and in contact with the support base. A pressure device, in fluid communication with the fluid passage, can exert a vacuum within the fluid passage to actively retain the microplate in the contact with the support base.
摘要翻译：真空辅助装置可以包括微孔板。微板可以包括第一表面和相对的第二表面。可以在微板的第一表面中形成多个孔。可以将多个孔中的每一个的尺寸设定为在其中接受测定。支撑基座可以包括流体通道。微板可以定位成与支撑基座相邻并与支撑基座接触。与流体通道流体连通的压力装置可以在流体通道内施加真空以主动地保持微板与支撑基座的接触。

2. 发明申请

US20160214102A1 Vacuum Assist for a Microplate 审中-公开
标题翻译：真空辅助微孔板
公开(公告)号：US20160214102A1
公开(公告)日：2016-07-28
申请号：US14968747
申请日：2015-12-14
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Mark F. Oldham , Adrian Fawcett
IPC分类号： B01L3/00
CPC分类号： B01L3/5085 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/545 , B01L7/52 , B01L9/50 , B01L9/523 , B01L9/56 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/14 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12Q1/686 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A vacuum assist apparatus can comprise a microplate. The microplate can comprise a first surface and an opposing second surface. A plurality of wells can be formed in the first surface of the microplate. Each of the plurality of wells can be sized to receive an assay therein. A support base can comprise a fluid passage. The microplate can be positioned adjacent and in contact with the support base. A pressure device, in fluid communication with the fluid passage, can exert a vacuum within the fluid passage to actively retain the microplate in the contact with the support base.
摘要翻译：真空辅助装置可以包括微孔板。微板可以包括第一表面和相对的第二表面。可以在微板的第一表面中形成多个孔。可以将多个孔中的每一个的尺寸设定为在其中接受测定。支撑基座可以包括流体通道。微板可以定位成与支撑基座相邻并与支撑基座接触。与流体通道流体连通的压力装置可以在流体通道内施加真空以主动地保持微板与支撑基座的接触。

3. 发明申请

US20160107160A1 Microfluidic Size-Exclusion Devices, Systems, and Methods 审中-公开
标题翻译：微流体尺寸排除装置，系统和方法
公开(公告)号：US20160107160A1
公开(公告)日：2016-04-21
申请号：US14887181
申请日：2015-10-19
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Sean M. Desmond , Zbigniew T. Bryning , John S. Shigeura , Gary Lim , Adrian Fawcett , Jacob K. Freudenthal , Gary Bordenkircher
IPC分类号： B01L3/00 , C12Q1/68
CPC分类号： B01L3/502715 , B01J19/0093 , B01L3/5027 , B01L3/502738 , B01L3/502753 , B01L7/52 , B01L2300/0681 , B01L2300/069 , B01L2300/0809 , B01L2300/0816 , B01L2300/0861 , B01L2300/0877 , B01L2300/123 , B01L2300/1805 , B01L2400/0633 , C12Q1/6844 , C12Q1/686 , G01N21/0303 , G01N2021/0346 , Y10T436/11 , Y10T436/143333 , Y10T436/17 , Y10T436/173845 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.
摘要翻译：提供微流体装置，组件和系统，以及操纵微尺寸流体样品的方法。还提供了具有多个专门处理特征的微流体装置。

4. 发明申请

US20140106364A1 Microfluidic Size-Exclusion Devices, Systems, and Methods 审中-公开
标题翻译：微流体尺寸排除装置，系统和方法
公开(公告)号：US20140106364A1
公开(公告)日：2014-04-17
申请号：US14137841
申请日：2013-12-20
申请人： Applied Biosystems, LLC
发明人： Sean M. Desmond , Zbigniew T. Bryning , John S. Shigeura , Gary Lim , Adrian Fawcett , Jacob K. Freudenthal , Gary Bordenkircher
IPC分类号： B01J19/00 , C12Q1/68
CPC分类号： B01L3/502715 , B01J19/0093 , B01L3/5027 , B01L3/502738 , B01L3/502753 , B01L7/52 , B01L2300/0681 , B01L2300/069 , B01L2300/0809 , B01L2300/0816 , B01L2300/0861 , B01L2300/0877 , B01L2300/123 , B01L2300/1805 , B01L2400/0633 , C12Q1/6844 , C12Q1/686 , G01N21/0303 , G01N2021/0346 , Y10T436/11 , Y10T436/143333 , Y10T436/17 , Y10T436/173845 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.
摘要翻译：提供微流体装置，组件和系统，以及操纵微尺寸流体样品的方法。还提供了具有多个专门处理特征的微流体装置。

5. 发明申请

US20130345097A1 Sample Block Apparatus and Method for Maintaining a Microcard on a Sample Block 审中-公开
标题翻译：样品块装置和在样品块上维持微卡的方法
公开(公告)号：US20130345097A1
公开(公告)日：2013-12-26
申请号：US13972735
申请日：2013-08-21
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Adrian Fawcett
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L7/52 , B01L9/523 , B01L2200/025 , B01L2300/0609 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2300/1805 , Y10S435/809
摘要： A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block. Methods of maintaining a microcard on a sample block of a thermal cycling device are also provided.
摘要翻译：一种用于热循环包含在具有顶表面和底表面的微卡中的生物材料样品的热循环装置。热循环装置可以包括具有被配置为接合微卡的底表面的上表面的样品块，真空装置和与样品块可操作地连接的温度控制系统。样品块的上表面可以包括多个通道，通道限定样品块和可以定位在其上的微卡的底表面之间的空间。真空装置可以与样品块流体连通，用于将气体从样品块中的通道限定的空间中排出。真空装置可以被配置为基本上保持样品块和微卡之间的真空，使得在微卡上施加保持力以将微卡推向样品块。还提供了在热循环装置的样品块上维持微卡的方法。

6. 发明授权

US10625258B2 Microfluidic size-exclusion devices, systems, and methods 有权
公开(公告)号：US10625258B2
公开(公告)日：2020-04-21
申请号：US14887181
申请日：2015-10-19
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Sean M. Desmond , Zbigniew T. Bryning , John S. Shigeura , Gary Lim , Adrian Fawcett , Jacob K. Freudenthal , Gary Bordenkircher
IPC分类号： B01L3/00 , B01J19/00 , C12Q1/6844 , C12Q1/686 , B01L7/00 , G01N21/03
CPC分类号： B01L3/502715 , B01J19/0093 , B01L3/5027 , B01L3/502738 , B01L3/502753 , C12Q1/686 , C12Q1/6844 , B01L7/52 , B01L2300/069 , B01L2300/0681 , B01L2300/0809 , B01L2300/0816 , B01L2300/0861 , B01L2300/0877 , B01L2300/123 , B01L2300/1805 , B01L2400/0633 , G01N21/0303 , G01N2021/0346 , Y10T436/11 , Y10T436/143333 , Y10T436/17 , Y10T436/173845 , Y10T436/25 , Y10T436/255 , Y10T436/2575 , Y10T436/25125 , Y10T436/25375
摘要： Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.

7. 发明授权

US10253361B2 Sample block apparatus and method for maintaining a microcard on a sample block 有权
公开(公告)号：US10253361B2
公开(公告)日：2019-04-09
申请号：US13972735
申请日：2013-08-21
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Adrian Fawcett
IPC分类号： C12M1/34 , C12Q1/686 , B01L7/00 , B01L9/00
摘要： A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block. Methods of maintaining a microcard on a sample block of a thermal cycling device are also provided.

8. 发明申请

US20160325284A1 Pressure Chamber Clamp Mechanism 审中-公开
标题翻译：压力室夹紧机构
公开(公告)号：US20160325284A1
公开(公告)日：2016-11-10
申请号：US14991844
申请日：2016-01-08
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Albert L. Camillo , Adrian Fawcett , Mike Y. Lu , Ian A. Harding
IPC分类号： B01L7/00 , C12P19/34
CPC分类号： B01L7/52 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/502723 , B01L3/50851 , B01L3/50853 , B01L9/523 , B01L9/56 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12P19/34 , G01N21/274 , G01N21/6452 , G01N35/028 , G01N2035/00148 , G01N2035/00356 , G01N2035/0405 , G01N2035/0425 , G01N2035/1037
摘要： In a high-density sequence detection system, an apparatus for transporting a microplate. A control system provides a thermocycler control signal to regulate a desired thermal output of a thermocycler block. A frame has a first side, a second side, and an opening that extends through the first side and the second side. A window is positioned in the opening and a circumferential seal is positioned around a periphery of the opening at the second side. The seal has a peripheral lip that seals against the microplate. A clamp is adapted to engage and displace the frame from an unclamped position to a clamped position, wherein in the unclamped position, the microplate is displaced away from the thermocycler block and in the clamped position, the clamp urges the microplate against the thermocycler block. In some embodiments the peripheral lip is positioned radially inward of the periphery of the opening.
摘要翻译：在高密度序列检测系统中，用于运送微孔板的装置。控制系统提供热循环器控制信号以调节热循环器块的期望的热输出。框架具有延伸穿过第一侧和第二侧的第一侧，第二侧和开口。窗口定位在开口中，并且周向密封件围绕第二侧的开口的周边定位。密封件具有密封微孔板的周边唇缘。夹具适于将框架从未夹紧位置接合并移位到夹紧位置，其中在未夹紧位置，微孔板远离热循环器块移动，并且在夹紧位置，夹具将微孔板推向热循环器块。在一些实施例中，外围唇缘位于开口周边的径向内侧。

9. 发明授权

US09213042B2 Vacuum assist for a microplate 有权
标题翻译：真空辅助微孔板
公开(公告)号：US09213042B2
公开(公告)日：2015-12-15
申请号：US14326243
申请日：2014-07-08
申请人： APPLIED BIOSYSTEMS, LLC
发明人： Mark F. Oldham , Adrian Fawcett
IPC分类号： G01N21/00 , G01N35/02 , B01L3/00 , G01N21/64 , C12Q1/68 , B01L3/02 , B01L7/00 , B01L9/00 , G01N21/27 , G01N35/00 , G01N35/10 , G01N35/04
CPC分类号： B01L3/5085 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/545 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/14 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , C12Q1/686 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A vacuum assist apparatus can comprise a microplate. The microplate can comprise a first surface and an opposing second surface. A plurality of wells can be formed in the first surface of the microplate. Each of the plurality of wells can be sized to receive an assay therein. A support base can comprise a fluid passage. The microplate can be positioned adjacent and in contact with the support base. A pressure device, in fluid communication with the fluid passage, can exert a vacuum within the fluid passage to actively retain the microplate in the contact with the support base.
摘要翻译：真空辅助装置可以包括微孔板。微板可以包括第一表面和相对的第二表面。可以在微板的第一表面中形成多个孔。可以将多个孔中的每一个的尺寸设定为在其中接受测定。支撑基座可以包括流体通道。微板可以定位成与支撑基座相邻并与支撑基座接触。与流体通道流体连通的压力装置可以在流体通道内施加真空以主动地保持微板与支撑基座的接触。

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