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1. 发明公开

US20230194470A1 ELECTROPHORETIC METHODS FOR SPATIAL ANALYSIS 审中-公开
公开(公告)号：US20230194470A1
公开(公告)日：2023-06-22
申请号：US18173458
申请日：2023-02-23
申请人： 10x Genomics, Inc.
发明人： Hanyoup Kim , Augusto Manuel Tentori , Rajiv Bharadwaj
IPC分类号： G01N27/447
CPC分类号： G01N27/44713
摘要： The present disclosure provides electrophoretic systems, methods and compositions for spatial analysis, which can serve to magnify or demagnify spatial resolution of analytes of interest that are captured using electrophoresis. Some implementations can use a diverging or converging electric field in an electrophoretic capture system. Such a divergent or convergent electric field, as opposed to a parallel electric field, can be generated by, for example, utilizing different sizes of electrodes associated with or imbedded in substrates. Also provided herein are electrophoretic systems, methods and compositions for spatial analysis, which can serve to selectively migrate one or more analytes from a region of interest in the biological sample for capture using electrophoresis.

2. 发明公开

US20230194469A1 ELECTROPHORESIS CASSETTES AND INSTRUMENTATION 审中-公开
公开(公告)号：US20230194469A1
公开(公告)日：2023-06-22
申请号：US17923436
申请日：2021-05-18
申请人： 10x Genomics, Inc.
发明人： Augusto Manuel Tentori , Hanyoup KIM , Felice Alessio Bava , Rajiv Bharadwaj
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/44782
摘要： Various implementations of electrophoretic systems and instruments are provided to improve electrophoresis and ease of use. Various electrophoretic systems and instruments utilize different electrode designs that can result in uniform electromigration of analytes. The electrophoretic systems and instrumentations optimize the size and/or location of electrodes relative to a sample, thereby increasing uniformity of electric field and reducing or minimizing drift of analyte migration. In addition, the electrophoresis systems and instruments provide a solution to conveniently check for electrical connections in the systems and instruments, and alert users of potential improper electrical connections, prior to performing electrophoresis.

3. 发明授权

US12071655B2 Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis 有权
公开(公告)号：US12071655B2
公开(公告)日：2024-08-27
申请号：US18311605
申请日：2023-05-03
申请人： 10x Genomics, Inc.
发明人： David Sukovich , Hanyoup Kim , Jerald Sapida , Spontaneous Rose McKnight Russell , Layla Katiraee , Augusto Manuel Tentori
IPC分类号： C12Q1/6841 , C12N15/10
CPC分类号： C12Q1/6841 , C12N15/1065
摘要： Provided herein are methods of enhancing spatial resolution of an analyte using sandwich maker system. The methods and systems used herein include a first substrate that includes a plurality of probes that include a capture domain and a spatial domain and a second substrate that includes a plurality of probes comprising a capture domain and a spatial domain.

4. 发明公开

US20240253036A1 FLUID DELIVERY METHODS 审中-公开
公开(公告)号：US20240253036A1
公开(公告)日：2024-08-01
申请号：US18585357
申请日：2024-02-23
申请人： 10x Genomics, Inc.
发明人： Hanyoup Kim , Augusto Manuel Tentori , Siyuan Xing , Rajiv Bharadwaj , Bill Kengli Lin , Felice Alessio Bava , Pratomo Putra Alimsijah , Nabil Mikhaiel
IPC分类号： B01L3/00 , C12Q1/6837 , C12Q1/6841 , G01N1/31
CPC分类号： B01L3/502715 , B01L3/502723 , C12Q1/6837 , C12Q1/6841 , G01N1/312 , B01L2200/027 , B01L2300/0822 , B01L2400/0421
摘要： Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

5. 发明公开

US20230416850A1 METHODS, COMPOSITIONS, AND SYSTEMS FOR DETECTING EXOGENOUS NUCLEIC ACIDS 审中-公开
公开(公告)号：US20230416850A1
公开(公告)日：2023-12-28
申请号：US18213088
申请日：2023-06-22
申请人： 10x Genomics, Inc.
发明人： Hardeep Pal Singh , Augusto Manuel Tentori
IPC分类号： C12Q1/70 , C12Q1/6818
CPC分类号： C12Q1/701 , C12Q1/6818 , C12Q1/6806
摘要： Provided herein are methods for capturing an exogenous probe and/or a capture handle sequence to a capture domain of a capture probe. Compositions, systems, and kits also are disclosed. In some instances, the methods include detecting an exogenous nucleic acid in a biological sample by hybridizing a first exogenous probe oligonucleotide and a second exogenous probe oligonucleotide to an exogenous nucleic acid in a biological sample on a first substrate, coupling the exogenous probe oligonucleotides, and capturing the coupled exogenous probe on a spatial array.

6. 发明公开

US20230203478A1 METHODS, COMPOSITIONS, AND SYSTEMS FOR CAPTURING PROBES AND/OR BARCODES 审中-公开
公开(公告)号：US20230203478A1
公开(公告)日：2023-06-29
申请号：US18178367
申请日：2023-03-03
申请人： 10x Genomics, Inc.
发明人： Hanyoup Kim , David Sukovich , Layla Katiraee , Augusto Manuel Tentori
IPC分类号： C12N15/10
CPC分类号： C12N15/1065
摘要： Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

7. 发明授权

US11680260B2 Methods, compositions, and systems for spatial analysis of analytes in a biological sample 有权
公开(公告)号：US11680260B2
公开(公告)日：2023-06-20
申请号：US17976422
申请日：2022-10-28
申请人： 10x Genomics, Inc.
发明人： Hanyoup Kim , David Sukovich , Layla Katiraee , Augusto Manuel Tentori , Lauren Gutgesell , Janine Hensel , Seayar Mohabbat
IPC分类号： C12Q1/6837 , C12N15/10
CPC分类号： C12N15/1065
摘要： Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

8. 发明申请

US20230135010A1 SEQUENTIAL ANALYTE CAPTURE 有权
公开(公告)号：US20230135010A1
公开(公告)日：2023-05-04
申请号：US17980248
申请日：2022-11-03
申请人： 10x Genomics, Inc.
发明人： Augusto Manuel Tentori , Rajiv Bharadwaj
IPC分类号： C12Q1/6837
摘要： Methods of capturing multiple analytes from a biological sample onto a substrate include: (a) positioning a first substrate that includes a plurality of capture probes with respect to a second substrate on which a biological sample that includes multiple analytes is disposed, where the first substrate is permeable; (b) delivering a first fluid to a first surface of the first substrate, where the first surface is opposite to a second surface of the first substrate that faces the second substrate; (c) allowing the first fluid to pass through the first substrate and contact the biological sample; (d) capturing a first one of the multiple analytes with one or more of the capture probes; (e) delivering a second fluid to the first surface of the first substrate; (f) allowing the second fluid to pass through the first substrate and contact the biological sample; and (g) capturing a second one of the multiple analytes with one or more of the capture probes. Other methods of capturing analytes from a biological sample are also described herein.

9. 发明申请

US20220025447A1 GENERATING SPATIAL ARRAYS WITH GRADIENTS 有权
公开(公告)号：US20220025447A1
公开(公告)日：2022-01-27
申请号：US17312238
申请日：2019-12-06
申请人： 10x Genomics, Inc.
发明人： Augusto Manuel Tentori , Marlon Stoeckius
IPC分类号： C12Q1/6837 , C12Q1/6841
摘要： The present disclosure relates to materials and methods for generating spatial arrays with gradients and using the generated spatial arrays to identify the location of analytes present in a biological sample.

10. 发明申请

US20210189475A1 IMAGING SYSTEM HARDWARE 有权
公开(公告)号：US20210189475A1
公开(公告)日：2021-06-24
申请号：US17172926
申请日：2021-02-10
申请人： 10x Genomics, Inc.
发明人： Augusto Manuel Tentori , Rajiv Bharadwaj , Felice Alessio Bava
IPC分类号： C12Q1/6837 , C12Q1/6841 , C12Q1/6874
摘要： A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

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