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1. 发明申请

US20050121368A1 Process for collecting seeds 审中-公开
标题翻译：收集种子的过程
公开(公告)号：US20050121368A1
公开(公告)日：2005-06-09
申请号：US10799418
申请日：2004-03-12
申请人： Yasushi Kohno , Yugo Nishiyama
发明人： Yasushi Kohno , Yugo Nishiyama
IPC分类号： A01D45/30 , A01C1/00 , B07B1/00
CPC分类号： A01C1/00
摘要： A process for collecting seeds efficiently and simply at a low cost from a lump consisting of a plurality of seeds having fluffy fibers is provided. The process for collecting seeds includes the steps of disentangling the lump consisting of a plurality of seeds and burning the fluffy fibers after the disentanglement of the lump.
摘要翻译：提供了从具有蓬松纤维的多个种子组成的块中以低成本有效且简单地收集种子的方法。收集种子的过程包括解开由多个种子组成的块并且在块的解体之后燃烧蓬松纤维的步骤。

2. 发明授权

US6112457A Process for preservation of germinated seeds 失效
标题翻译：保存发芽种子的方法
公开(公告)号：US6112457A
公开(公告)日：2000-09-05
申请号：US965571
申请日：1997-11-06
申请人： Yasushi Kohno , Takamichi Maejima , Kazuhiro Inose , Yugo Nishiyama
发明人： Yasushi Kohno , Takamichi Maejima , Kazuhiro Inose , Yugo Nishiyama
IPC分类号： A01C1/06
CPC分类号： A01C1/06
摘要： A seed 9 is held in an aqueous gel 7 so as to constitute a seed covered with gel S. Seed covered with gel S is dried after seed 9 germinates. Otherwise, a through hole 12a is pierced vertically within a gel culture medium 12, a seed 13 is inserted into trough hole 12a, and gel culture medium 12 including seed 13 is dried after seed 13 germinates.
摘要翻译：将种子9保持在水性凝胶7中以构成覆盖有凝胶S的种子。种子9发芽后将用凝胶S覆盖的种子干燥。否则，在凝胶培养基12内垂直穿透通孔12a，将晶种13插入槽孔12a中，将种子13的凝胶培养基12在种子13发芽后干燥。

3. 发明授权

US5875899A Apparatus for classifying objects by sedimentation 失效
标题翻译：用于通过沉降对物体进行分类的装置
公开(公告)号：US5875899A
公开(公告)日：1999-03-02
申请号：US674793
申请日：1996-07-03
申请人： Yugo Nishiyama
发明人： Yugo Nishiyama
IPC分类号： B03B5/28 , B01D19/00 , B03B5/62 , B03B5/64 , C12M1/26 , G01N15/04 , B03B5/66
CPC分类号： B03B5/62 , B01D19/0031 , C12M47/04 , G01N15/04
摘要： In an apparatus for classifying objects by sedimentation having a straightening passage, capable of being prevented adhesion of bubbles to the straightening passage, and capable of being prevented a sample supplying error generated caused by a closed sample supplying portion on account of air locking, there is provided a bubble-removing passage for removing the bubbles within fluids at upper stream position of a straightening mesh within a classifying bath for classifying objects based on dropping position caused by a final sinking velocity of said objects falling within fluids after throwing said objects into flowing fluids, the bubble-removing passage which comprises a filter and a filter holder secured within the classifying bath through a protuberance.
摘要翻译：在具有矫直通道的沉淀物分类装置中，能够防止气泡粘附到矫直通道上，并且能够防止由于空气锁定而导致的由封闭的样品供应部分产生的样品供给错误，提供了一个气泡去除通道，用于去除分级槽内的矫直网的上流位置的流体内的气泡，用于基于在将物体投入流动流体之后落入流体内的所述物体的最终下沉速度引起的落下位置分类物体气泡去除通道包括过滤器和通过突起固定在分级浴中的过滤器保持器。

4. 发明授权

US5665226A Substance collecting apparatus for precipitation classifying device 失效
标题翻译：用于沉淀分级装置的物质采集装置
公开(公告)号：US5665226A
公开(公告)日：1997-09-09
申请号：US510304
申请日：1995-08-02
申请人： Yugo Nishiyama
发明人： Yugo Nishiyama
IPC分类号： B03B5/28 , B01D21/00 , B03B5/60 , B03B5/62 , B03B11/00 , B01D17/12 , B03B5/00
CPC分类号： B03B11/00 , B01D21/0024 , B01D21/245 , B01D21/2488 , B01D21/302 , B01D21/34 , B03B5/60 , B03B5/62 , Y10T137/86187
摘要： The object of the present invention is to provide a substance collecting apparatus for a precipitation classifying device which is capable of automatically transmitting the classified substances to a next process in a sterile atmosphere even during a substance classifying operation is being performed, and in order to attain the object, the substance collecting apparatus comprises a pipe 15 whose one end is connected with a partitioned part 6 of a classifying tank side by way of a first electrovalve, while the other end is diverged into two ways, one of which is connected with a liquid-substance separating tube and having a third electrovalve 23 in the halfway thereof, whereas the other is connected with an exhausting side of a pump 24 and having a second electrovalve 22. The intake side of the pump 24 is further connected with a liquid reservoir tank 25, having in the halfway of the first pipe 15 a liquid flux regulating valve 32. The far end of a second pipe 16 connected with the upper side of the liquid-substance separating tube 29 is submerged in the liquid reservoir tank 25, and a vertically lower portion of the liquid-substance separating tube 29 is connected with a third pipe 17, in which a fourth electrovalve 30 and a fifth electrovalve 31 are disposed having an interval therebetween.
摘要翻译：本发明的目的是提供一种用于沉淀分选装置的物质收集装置，其能够即使在进行物质分类操作期间也能够在无菌气氛中将分类物质自动传送到下一个处理，并且为了获得该物品收集装置包括管15，其一端通过第一电阀与分级罐侧的分隔部分6连接，而另一端分叉成两种方式，其中一个与液体物质分离管，并且在其一半中具有第三电阀23，而另一个与泵24的排气侧连接并具有第二电阀22.泵24的进气侧还与液体储存器在第一管15的中途具有液体流量调节阀32的罐25，与第一管16相连的远端液体分离管29的侧面淹没在储液箱25中，液体分离管29的垂直下部与第三管17连接，其中第四电阀30和第五电阀 31设置有间隔。

5. 发明授权

US5720394A Sample supply unit for settling classification system 失效
标题翻译：用于沉降分级系统的样品供应单元
公开(公告)号：US5720394A
公开(公告)日：1998-02-24
申请号：US498496
申请日：1995-07-05
申请人： Yugo Nishiyama
发明人： Yugo Nishiyama
IPC分类号： B03B5/64 , B03B5/60 , B03B5/62 , B03B11/00 , C12M1/36
CPC分类号： C12M47/04 , B03B5/60 , B03B5/623 , C12M47/02
摘要： A tube-type sample supplying portion 1 is provided upright above a classification tank 2. A sample inlet pipe 5, which communicating with a culture tank 3, is connected to the lower part of the sample supplying portion 1. An outlet pipe 6, which communicating with the culture tank 3, is connected to the upper part of the sample supplying portion 1. A sample feed pump 4 and a valve 7 are positioned in one of the flow passages between the outlet pipe 6 and the culture tank 3. The flow speed of the fluid including the sample in the sample supplying portion 1 is set to a speed being only a little faster than the final sinking speed of lumps of immature cultivated cells in the fluid.
摘要翻译：在分级罐2的上方设置有管状的试样供给部1，与培养槽3连通的试样导入管5与试样供给部1的下部连接。出口管6 与培养槽3连通，连接到样品供给部1的上部。样品供给泵4和阀7位于出口管6和培养槽3之间的一个流路中。流动将包含样品供给部1中的样品的流体的速度设定为比流体中的未成熟培养细胞块的最终下沉速度稍快的速度。

6. 发明授权

US06572809B1 Gel coating method and apparatus 失效
标题翻译：凝胶涂布方法和装置
公开(公告)号：US06572809B1
公开(公告)日：2003-06-03
申请号：US09959369
申请日：2001-10-17
申请人： Yugo Nishiyama , Yasushi Kohno
发明人： Yugo Nishiyama , Yasushi Kohno
IPC分类号： B29C3910
CPC分类号： A01C1/06
摘要： A gel coating method comprising making a mold (27), which has both a gelatinizer impermeability or a slight gelatinizer impermeability and a hardening agent permeability, retain a hardening agent (26) thereon beforehand by immersing the mold (27) in the hardening agent (26) and taking out the mold (27) therefrom, placing a gelatinizer (30) and an object to be sealed (9) in the resultant mold (27), allowing the hardening agent (26) to permeate through the mold (27) as a whole by immersing the mold in the hardening agent (26) again, and uniformly hardening the gelatinizer (30), which is around the object to be sealed (9), from the portion thereof which contacts the mold (27), whereby the object to be sealed (9) is rendered easily removable as gel-coated object (9a) from the mold (27).
摘要翻译：凝胶涂布方法包括制备具有凝胶化剂不渗透性或轻微凝胶化剂不渗透性和硬化剂渗透性的模具（27），其中通过将模具（27）浸入硬化剂中来预先固定硬化剂（26） 26）并从其中取出模具（27），将糊化剂（30）和要密封的物体（9）放置在所得模具（27）中，使硬化剂（26）渗透通过模具（27）通过将模具再次浸渍在硬化剂（26）中，并将其与被密封物（9）周围的凝胶化剂（30）从与模具（27）接触的部分均匀地硬化，待密封物体9从模具27易于作为凝胶涂覆物体9a移动。

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