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1. 发明授权

US09567346B2 Biotin derivatives 有权
标题翻译：生物素衍生物
公开(公告)号：US09567346B2
公开(公告)日：2017-02-14
申请号：US13881518
申请日：2011-10-28
申请人： Lai-Qiang Ying , Bruce Branchaud , Yu-Zhong Zhang , Stephen Yue
发明人： Lai-Qiang Ying , Bruce Branchaud , Yu-Zhong Zhang , Stephen Yue
IPC分类号： C07D495/04 , C08B37/00 , G01N33/532 , G01N33/543 , G01N33/58
CPC分类号： C07D495/04 , C07B2200/11 , C07D403/12 , C07K1/13 , C07K1/22 , C07K16/42 , C07K2317/20 , C07K2317/92 , C08B37/00 , C09B11/24 , C09K11/06 , C09K2211/1007 , C09K2211/1088 , C12N5/0636 , C12N5/0637 , C12N5/0638 , G01N33/532 , G01N33/533 , G01N33/54353 , G01N33/58 , G01N33/582 , G01N33/6803
摘要： Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.
摘要翻译：生物素衍生物，使用生物素衍生物的方法和包含生物素衍生物的试剂盒。

2. 发明申请

US20130020507A1 Methods for Detecting Defects in Inorganic-Coated Polymer Surfaces 审中-公开
标题翻译：检测无机涂层聚合物表面缺陷的方法
公开(公告)号：US20130020507A1
公开(公告)日：2013-01-24
申请号：US13633713
申请日：2012-10-02
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： G01N21/64 , B32B3/30 , C07F5/02
CPC分类号： G01N21/91 , C07F5/022 , Y10T428/24355
摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.
摘要翻译：亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。使用所述方法会使表面缺陷出现荧光，而其余表面未标记。所公开的方法是便宜，快速和容易的替代现有方法。

3. 发明申请

US20120064012A1 Uniform Fluorescent Microspheres with Hydrophobic Surfaces 审中-公开
标题翻译：均匀荧光微球与疏水表面
公开(公告)号：US20120064012A1
公开(公告)日：2012-03-15
申请号：US13209770
申请日：2011-08-15
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61K49/0021 , A61K49/0004 , A61K49/0093
摘要： Fluorescent microspheres for the measurement of blood flow are provided. The microspheres are substantially uniform in diameter and have a hydrophobic surface, which allows them to circulate more freely throughout bloodstream, while reducing immunogenicity, particle aggregation and bioaccumulation. The hydrophobic surface on each microsphere is generally comprised of polymeric material having a limited surface charge.
摘要翻译：提供了用于测量血流量的荧光微球。微球的直径基本上是均匀的并且具有疏水性表面，这允许它们在血流中更自由地循环，同时降低免疫原性，颗粒聚集和生物累积。每个微球体上的疏水表面通常由具有有限表面电荷的聚合物材料组成。

4. 发明申请

US20100291685A1 METHODS FOR DETECTING DEFECTS IN INORGANIC-COATED POLYMER SURFACES 有权
标题翻译：用于检测无机涂层聚合物表面缺陷的方法
公开(公告)号：US20100291685A1
公开(公告)日：2010-11-18
申请号：US12808325
申请日：2008-12-17
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： G01N31/00
CPC分类号： G01N21/91 , G01N21/643 , G01N21/6489 , G01N21/94 , G01N2021/646 , G01N2021/8427
摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.
摘要翻译：亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。使用所述方法会使表面缺陷出现荧光，而其余表面未标记。所公开的方法是便宜，快速和容易的替代现有方法。

5. 发明授权

US5686261A Xanthylium dyes that are well retained in mitochondria 失效
标题翻译：良好保留在线粒体中的Xanthylium染料
公开(公告)号：US5686261A
公开(公告)日：1997-11-11
申请号：US383298
申请日：1995-02-03
申请人： Yu-Zhong Zhang , Richard P. Haugland
发明人： Yu-Zhong Zhang , Richard P. Haugland
IPC分类号： C07D311/82 , C09B11/12 , C12Q1/02 , C12Q1/68
CPC分类号： C09B11/12 , C07D311/82
摘要： The present invention describes a method of staining mitochondria, and analyzing mitochondrial function, using a class of fluorescent substituted 3',6'-diaminoxanthenes and their reduced analogs 3',6'-diaminodihydroxanthenes, which are oxidized to the fluorescent form of the dye in situ. In their oxidized form, the dyes selectively localize within mitochondria. The dyes of the invention are substituted by an alkylating group that allows their retention in mitochondria even after cell death, fixation, and permeabilization.
摘要翻译：本发明描述了使用一类荧光取代的3'，6'-二氨基呫吨及其还原的类似物3'，6'-二氨基二羟基呫吨染色线粒体并分析线粒体功能的方法，其被氧化成染料的荧光形式在原地。在其氧化形式中，染料选择性地定位在线粒体内。本发明的染料被烷基化基团取代，即使在细胞死亡，固定和透化后也能使它们保留在线粒体中。

6. 发明授权

US5242805A Long wavelength lipophilic fluorogenic glycosidase substrates 失效
标题翻译：长波长磷酸荧光蛋白酶底物
公开(公告)号：US5242805A
公开(公告)日：1993-09-07
申请号：US749255
申请日：1991-08-23
申请人： John J. Naleway , Yu-zhong Zhang , Richard P. Haugland
发明人： John J. Naleway , Yu-zhong Zhang , Richard P. Haugland
IPC分类号： C07H17/00 , C12Q1/34
CPC分类号： C12Q1/34 , C07H17/00 , C12Q2334/00 , G01N2333/924
摘要： The claimed invention relates to a substrate for evaluating glycosidic enzymes comprising a resorufin derivative of the general formula: ##STR1## wherein Gly is a carbohydrate bonded to resorufin by a glycosidic linkage; where at least one of substituents R.sub.1, R.sub.2, R.sub.4, R.sub.6, R.sub.8, and R.sub.9 is a lipophilic residue of the formula --L(CH.sub.2).sub.n CH.sub.3, where n is greater than 3 and less than 22, and where L is a methylene --CH.sub.2 --, an amide --NHCO--, a sulfonamide --NHSO.sub.2 --, a carboxamide --CONH--, a carboxylate ester --COO--, a urethane --NHCOO--, a urea --NHCONH--, or a thiourea --NHCSNH--; andwhere the remainder of substituents R.sub.1, R.sub.2, R.sub.4, R.sub.6, R.sub.8, and R.sub.9, which may be the same or different, are hydrogen, halogen, or other lipophilic residues, which may be the same or different, containing from about 1 to about 22 carbon atoms of the formula --L'(CH.sub.2).sub.m CH.sub.3, where m is less than 22, and where L' is a methylene --CH.sub.2 --, an amide --NHCO--, a sulfonamide --NHSO.sub.2 --, a carboxamide --CONH--, a carboxylate ester --COO--, a urethane --NHCOO--, a urea --NHCONH--, or a thiourea --NHCSNH--.A preferred embodiment of the invention is a non-fluorescent substrate specifically hydrolyzable by a glycosidase inside a cell to yield, after greater than about 2 minutes, an orange to red fluorescent detection product which is retained inside a viable cell more than about 2 hours at greater than about 15.degree. C. and which is non-toxic to the cell. The substrates are used for evaluating a glycosidic enzyme in living plant or animal cells whether the enzyme is present endogenously; present as a result of manipulation of the cell's genome, or added to the cell exogenously, such as by covalently binding the enzyme to a protein to form an enzyme-protein complex that enters the cell.
摘要翻译：所要求保护的发明涉及用于评估糖苷酶的底物，其包含通式如下的再生烯衍生物：其中Gly是通过糖苷键键合至残留的碳水化合物; 其中取代基R 1，R 2，R 4，R 6，R 8和R 9中的至少一个是式-L（CH 2）n CH 3的亲脂性残基，其中n大于3且小于22，并且其中L是亚甲基 - CH 2 - ，酰胺-NHCO-，磺酰胺-NHSO 2 - ，酰胺-CONH-，羧酸酯-COO-，氨基甲酸酯-NHCOO-，脲-NHCONH-或硫脲-NHCSNH-; 并且其中可以相同或不同的取代基R 1，R 2，R 4，R 6，R 8和R 9的其余部分是氢，卤素或可以相同或不同的其它亲脂性残基，其含有约1至约22个碳原子的式-L'（CH 2）m CH 3，其中m小于22，其中L'是亚甲基-CH 2 - ，酰胺-NHCO-，磺酰胺-NHSO 2 - ，甲酰胺-CONH- ，羧酸酯-COO-，氨基甲酸酯-NHCOO-，脲-NHCONH-或硫脲-NHCSNH-。本发明的优选实施方案是特异性可由细胞内的糖苷酶水解的非荧光底物，大于约2分钟后，产生橙色至红色荧光检测产物，其在活细胞内保留超过约2小时大于约15℃，对细胞无毒性。底物用于评价活体植物或动物细胞中的糖苷酶，无论酶是否内源性存在; 作为细胞基因组的操作或外源性加入到细胞中的结果，例如通过将酶与蛋白质共价结合以形成进入细胞的酶 - 蛋白质复合物。

7. 发明授权

US08304242B2 Methods for detecting defects in inorganic-coated polymer surfaces 有权
标题翻译：检测无机涂层聚合物表面缺陷的方法
公开(公告)号：US08304242B2
公开(公告)日：2012-11-06
申请号：US12808325
申请日：2008-12-17
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： G01N31/22
CPC分类号： G01N21/91 , G01N21/643 , G01N21/6489 , G01N21/94 , G01N2021/646 , G01N2021/8427
摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.
摘要翻译：亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。使用所述方法会使表面缺陷出现荧光，而其余表面未标记。所公开的方法是便宜，快速和容易的替代现有方法。

8. 发明申请

US20120276015A1 Optical in vivo Imaging Contrast Agents and Methods of Use 审中-公开
标题翻译：光学体内成像对比剂和使用方法
公开(公告)号：US20120276015A1
公开(公告)日：2012-11-01
申请号：US13344224
申请日：2012-01-05
申请人： John Mauro , Julie Nyhus , Thomas Steinberg , Yu-Zhong Zhang
发明人： John Mauro , Julie Nyhus , Thomas Steinberg , Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61B5/0059 , A61K49/0021 , A61K49/0093
摘要： Provided is an optical in vivo contrast agent comprising a fluorescent polymeric microsphere, wherein the micro-sphere is impregnated with a dye having an excitation and emission spectrum compatible with in vivo imaging, and wherein the microsphere is coated with a block copolymer.
摘要翻译：提供了一种包含荧光聚合物微球的光学体内造影剂，其中所述微球浸渍有具有与体内成像相容的激发和发射光谱的染料，并且其中所述微球涂覆有嵌段共聚物。

9. 发明申请

US20080019921A1 UNIFORM FLUORESCENT MICROSPHERE WITH HYDROPHOBIC SURFACES 审中-公开
标题翻译：具有疏水性表面的均匀荧光显微镜
公开(公告)号：US20080019921A1
公开(公告)日：2008-01-24
申请号：US11772063
申请日：2007-06-29
申请人： Yu-Zhong Zhang
发明人： Yu-Zhong Zhang
IPC分类号： A61K49/00
CPC分类号： A61K49/0021 , A61K49/0004 , A61K49/0093
摘要： Fluorescent microspheres for the measurement of blood flow are provided. The microspheres are substantially uniform in diameter and have a hydrophobic surface, which allows them to circulate more freely throughout bloodstream, while reducing immunogenicity, particle aggregation and bioaccumulation. The hydrophobic surface on each microsphere is generally comprised of polymeric material having a limited surface charge.
摘要翻译：提供了用于测量血流量的荧光微球。微球的直径基本上是均匀的并且具有疏水性表面，这允许它们在血流中更自由地循环，同时降低免疫原性，颗粒聚集和生物累积。每个微球体上的疏水表面通常由具有有限表面电荷的聚合物材料组成。

10. 发明授权

US5869689A Stains for acidic organelles 失效
标题翻译：酸性细胞器污渍
公开(公告)号：US5869689A
公开(公告)日：1999-02-09
申请号：US544226
申请日：1995-10-17
申请人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
发明人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
IPC分类号： C07F5/02 , G01N1/30 , G01N33/58 , C07D207/00
CPC分类号： G01N33/582 , C07F5/022 , G01N1/30
摘要： Dipyrrometheneboron difluoride dyes possessing a covalently attached basic amine moiety are described that have utility for staining acidic organelles. Samples comprising isolated acidic organelles, or a cell or cells containing acidic organelles, are stained by preparing an aqueous labeling solution of the dye and incubating the sample in the labeling solution for a time sufficient to produce fluorescent labeled acidic organelles.
摘要翻译：描述了具有共价连接的碱性胺部分的二吡咯亚甲基二氟化物染料，其具有用于染色酸性细胞器的效用。包含分离的酸性细胞器或含有酸性细胞器的细胞或细胞的样品通过制备染料的水性标记溶液并将样品在标记溶液中孵育足以产生荧光标记的酸性细胞器的时间来染色。

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