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1. 发明授权

US5869689A Stains for acidic organelles 失效
标题翻译：酸性细胞器污渍
公开(公告)号：US5869689A
公开(公告)日：1999-02-09
申请号：US544226
申请日：1995-10-17
申请人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
发明人： Yu-Zhong Zhang , Zhenjun Diwu , Richard P. Haugland
IPC分类号： C07F5/02 , G01N1/30 , G01N33/58 , C07D207/00
CPC分类号： G01N33/582 , C07F5/022 , G01N1/30
摘要： Dipyrrometheneboron difluoride dyes possessing a covalently attached basic amine moiety are described that have utility for staining acidic organelles. Samples comprising isolated acidic organelles, or a cell or cells containing acidic organelles, are stained by preparing an aqueous labeling solution of the dye and incubating the sample in the labeling solution for a time sufficient to produce fluorescent labeled acidic organelles.
摘要翻译：描述了具有共价连接的碱性胺部分的二吡咯亚甲基二氟化物染料，其具有用于染色酸性细胞器的效用。包含分离的酸性细胞器或含有酸性细胞器的细胞或细胞的样品通过制备染料的水性标记溶液并将样品在标记溶液中孵育足以产生荧光标记的酸性细胞器的时间来染色。

2. 发明授权

US5773236A Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules 失效
标题翻译：用于酶测定的多卤代芳基取代的报道分子
公开(公告)号：US5773236A
公开(公告)日：1998-06-30
申请号：US845301
申请日：1997-04-25
申请人： Zhenjun Diwu , Richard P. Haugland
发明人： Zhenjun Diwu , Richard P. Haugland
IPC分类号： C07C233/65 , C07C233/78 , C07D265/38 , C07D295/192 , C07D311/16 , C07D311/88 , C07D493/10 , C07F9/655 , C07F9/6561 , C07K5/02 , A61K38/06 , C07D311/82
CPC分类号： C07D295/192 , C07C233/65 , C07C233/78 , C07D265/38 , C07D311/16 , C07D311/88 , C07D493/10 , C07F9/65522 , C07F9/6561 , C07K5/0215
摘要： The subject invention describes compounds containing a polyhalogenated aryl moiety. The compounds of the invention are particularly useful for the assay of a variety of enzymes, including intracellular enzymes. The subject invention also describes assays for glutathione and/or glutathione transferase enzymes. Selected compounds of the invention are particularly useful for improving the retention of fluorescent products of enzyme metabolism in cells.
摘要翻译：本发明描述了含有多卤代芳基部分的化合物。本发明的化合物特别可用于各种酶的测定，包括细胞内酶。本发明还描述了对谷胱甘肽和/或谷胱甘肽转移酶的测定。本发明的选择的化合物特别可用于改善细胞中酶代谢的荧光产物的保留。

3. 发明申请

US20110117659A1 QUINAZOLINE-CONTAINING KITS FOR LABELING ALDEHYDE OR KETONE MOIETIES 审中-公开
标题翻译：用于标示醛或酮类的含喹诺酮的包装盒
公开(公告)号：US20110117659A1
公开(公告)日：2011-05-19
申请号：US12906014
申请日：2010-10-15
申请人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
发明人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
IPC分类号： G01N33/68 , G01N33/00
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R5是-L-Z; L是亚芳基或C1-6全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-6烷基，C1-6烷氧基，C1-6全氟烷基，C1-6烷基氨基，二（C2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基，或磺基; 和R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基，磺基或-LZ。描述了用衍生化试剂处理样品的方法。这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

4. 发明授权

US06967251B2 Reagents for labeling biomolecules having aldehyde or ketone moieties 有权
标题翻译：用于标记具有醛或酮部分的生物分子的试剂
公开(公告)号：US06967251B2
公开(公告)日：2005-11-22
申请号：US09970215
申请日：2001-10-02
申请人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
发明人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
IPC分类号： G01N33/532 , C07D239/91 , C07D239/94 , C07D249/08 , C07D263/60 , C07D265/22 , C07D277/66 , C07D401/04 , G01N33/58 , C12Q1/68 , G01N1/30
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 2-12烷基）氨基，氨基，羧基，氰基，卤素，C 1-6烷基氨基，羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基，磺基或-LZ。描述了用衍生化试剂处理样品的方法。这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

5. 发明授权

US06962992B2 Crown ether derivatives 失效
标题翻译：冠醚衍生物
公开(公告)号：US06962992B2
公开(公告)日：2005-11-08
申请号：US10026302
申请日：2001-12-19
申请人： Vladimir V. Martin , Kyle R. Gee , Richard P. Haugland , Zhenjun Diwu
发明人： Vladimir V. Martin , Kyle R. Gee , Richard P. Haugland , Zhenjun Diwu
IPC分类号： C07D273/00 , C07H21/00 , C09B57/10 , C09B69/00 , G01N33/58 , G01N33/84 , C07D413/04 , C07D273/08 , C07D317/28
CPC分类号： A61K49/0052 , A61K49/0021 , A61K49/0041 , C07D273/00 , C07H21/00 , C09B11/24 , C09B23/04 , C09B57/00 , C09B57/10 , C09B69/00 , G01N33/582 , G01N33/84
摘要： The invention describes crown ether chelators, including crown ethers having the formula and aza-substituted and thia-substituted analogs thereof. These crown ethers are substituted by a dye moiety, a chemically reactive group, a conjugated substance, or a combination thereof. Chelators that are substituted by fluorescent dyes are particularly useful as indicators for metal cations, particularly Na+ and K+ ions, and particularly where binding of the target ion results in a change in the fluorescence properties of the indicator that can be correlated with the ion concentration. Methods are provided for utilizing reactive groups on the chelators for conjugation to dyes, lipids and polymers and methods for enhancing entry of the indicators into living cells.
摘要翻译：本发明描述了冠醚螯合剂，包括具有下式的冠醚和其氮杂取代和硫取代的类似物。这些冠醚被染料部分，化学反应性基团，共轭物质或其组合取代。被荧光染料取代的螯合剂特别可用作金属阳离子，特别是Na +和K + +离子的指示剂，特别是当目标离子的结合导致变化时在可以与离子浓度相关的指示剂的荧光特性中。提供了利用螯合剂上的反应性基团与染料偶联的方法，脂质和聚合物以及用于增强指示剂进入活细胞的方法。

6. 发明授权

US06716979B2 Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings 有权
标题翻译：含稠环的1,2-二氢-7-羟基喹啉的衍生物
公开(公告)号：US06716979B2
公开(公告)日：2004-04-06
申请号：US09922333
申请日：2001-08-04
申请人： Zhenjun Diwu , Jiaxing Liu , Richard P. Haugland , Kyle R. Gee
发明人： Zhenjun Diwu , Jiaxing Liu , Richard P. Haugland , Kyle R. Gee
IPC分类号： C07D49122
CPC分类号： C07D471/04 , C07D491/04 , C07D495/04 , C07D495/14 , G01N33/582 , Y10S436/80
摘要： The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
摘要翻译：本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。本发明的许多染料是有用的荧光染料。本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其它分析物中的用途。

7. 发明授权

US06316267B1 Luminescent protein stains an their method of use 有权
标题翻译：发光蛋白染色其使用方法
公开(公告)号：US06316267B1
公开(公告)日：2001-11-13
申请号：US09429739
申请日：1999-10-27
申请人： Mahesh K. Bhalgat , Zhenjun Diwu , Richard P. Haugland , Wayne F. Patton
发明人： Mahesh K. Bhalgat , Zhenjun Diwu , Richard P. Haugland , Wayne F. Patton
IPC分类号： G01N3348
CPC分类号： G01N33/6803 , G01N33/533 , G01N33/582 , G01N33/68 , G01N33/683 , G01N33/6845 , G01N33/6851 , Y10T436/25125 , Y10T436/25375
摘要： The invention relates to the staining of poly(amino acids), including peptides, polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals.
摘要翻译：本发明涉及使用过渡金属的中性或阴离子复合物在凝胶和固体支持物上染色聚（氨基酸），包括肽，多肽和蛋白质。

8. 发明授权

US07816527B2 Quinazoline-containing kits for labeling aldehyde or ketone moieties 有权
标题翻译：用于标记醛或酮部分的含喹唑啉试剂盒
公开(公告)号：US07816527B2
公开(公告)日：2010-10-19
申请号：US11182122
申请日：2005-07-15
申请人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
发明人： Richard P. Haugland , Thomas H. Steinberg , Wayne F. Patton , Zhenjun Diwu
IPC分类号： C07D239/72
CPC分类号： C07D265/22 , C07D239/91 , Y10T436/143333 , Y10T436/200833
摘要： Kits including novel fluorescent derivitization reagents for labeling an aldehyde- and/or ketone-containing target substance in a sample are described. In one embodiment kits are provided the novel fluorescent derivitization reagents of which have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-16 alkyl, C1-6 alkylamino, amino, or halogen; and R21-R24 are independently H, C 1-6 alkyl, C 1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The kits and reagents described are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
摘要翻译：描述了包括用于标记样品中含醛和/或酮的目标物质的新型荧光衍生试剂的试剂盒。在一个实施方案中提供试剂盒，其新型荧光衍生试剂具有下式：其中Q为羰基，硫代羰基或磺酰基，R5为-L-Z; L是亚芳基或C1-6全氟亚烷基; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R11-R14独立地为H，C1-16烷基，C1-6烷基氨基，氨基或卤素; R 21 -R 24独立地为H，C 1-6烷基，C 1-6烷氧基，C 1-6全氟烷基，C 1-6烷基氨基，二（C 2-12 - 烷基）氨基，氨基，羧基，氰基，卤素，羟基，硝基，苯基，磺基或-LZ。所述的试剂盒和试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

9. 发明授权

US08258292B2 Methods of making dyes 有权
标题翻译：染色方法
公开(公告)号：US08258292B2
公开(公告)日：2012-09-04
申请号：US12287074
申请日：2008-10-06
申请人： Zhenjun Diwu , Jianheng Zhang , Yi Tang , Xiang Guobing
发明人： Zhenjun Diwu , Jianheng Zhang , Yi Tang , Xiang Guobing
IPC分类号： C07D487/14
CPC分类号： C09B23/06 , C07D487/14 , C07D487/22 , C07D498/22 , C09B23/0066 , C09B23/02 , G01N33/52 , G01N33/533 , Y10T436/143333
摘要： Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.
摘要翻译：描述了在1位和3'-位之间分子内交联的化学反应性碳菁染料，它们的生物缀合物及其用途。 1,3'-交联的碳氰酸酯优于光谱相似的1,1'-交联或非交联染料的共轭物。本发明包括具有一个或多个苯并氮的衍生化合物。

10. 发明申请

US20120123109A1 Luminescence quenching compounds 有权
标题翻译：发光淬灭化合物
公开(公告)号：US20120123109A1
公开(公告)日：2012-05-17
申请号：US13374728
申请日：2012-01-09
申请人： Zhenjun Diwu , Jianheng Zhang , Yi Tang
发明人： Zhenjun Diwu , Jianheng Zhang , Yi Tang
IPC分类号： C07H21/00 , C07D409/14
CPC分类号： C07D409/14 , C07D209/60 , C07D403/14 , C07D409/04 , C07D409/06 , C07D417/14 , C07D471/04 , C07H21/04 , C07J43/00 , G01N33/542 , G01N33/582
摘要： The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.
摘要翻译：本发明的淬灭化合物是被一个或多个杂芳族猝灭部分取代的弱发光花青素。本发明的淬灭化合物表现出很少或不可观察到的发光，并有效地淬灭广谱的发光化合物。化学反应猝灭化合物具有用于标记各种物质（包括生物分子）的效用。这些标记的物质对于各种能量转移测定和应用是非常有用的。

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