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1. 发明授权

US6139757A Method of separating cells from blood using a filter having a changeable porosity 失效
标题翻译：使用具有可变孔隙率的过滤器从血液中分离细胞的方法
公开(公告)号：US6139757A
公开(公告)日：2000-10-31
申请号：US999529
申请日：1997-03-28
申请人： Yoshitaka Ohmura , Noboru Taguchi , Tadashi Sameshima
发明人： Yoshitaka Ohmura , Noboru Taguchi , Tadashi Sameshima
IPC分类号： A61M1/00 , A61M1/34 , B01D29/01 , B01D61/18 , B01D63/08 , B01D69/02 , B01D69/12 , C12M3/06 , G01N1/10 , B01D25/26
CPC分类号： B01D69/12 , B01D61/18 , B01D69/02 , G01N33/491
摘要： A method of separating cells from blood by using a filter apparatus having first and second ports and a filter member therebetween having a changeable porosity. The filter member includes a stack of porous polymer sheets that preferably has a gradient of a physical or chemical characteristic in the direction of stacking. This can include a gradient in porosity, hydrophilicity or zeta potential. In a first step, blood is filtered through the apparatus when the filter member is set to a compressive state having a first porosity capable of capturing the objective cells. In a later step, the filter member is then set to a non-compressive state having a second porosity, higher than the first, for recovering the objective cells. The change in porosity results from the enlargement or reduction of the diameter of the pores of the filter member. The method can also include washing the filter member when the member is set to a porosity that is higher than the first porosity but lower than the second porosity.
摘要翻译：通过使用具有第一和第二端口的过滤装置和其间具有可变孔隙率的过滤器构件从血液中分离细胞的方法。过滤构件包括一堆多孔聚合物片，其优选地具有在堆叠方向上的物理或化学特性的梯度。这可以包括孔隙度，亲水性或ζ电位的梯度。在第一步骤中，当过滤构件被设置为具有能够捕获目标单元的第一孔隙的压缩状态时，通过设备过滤血液。在随后的步骤中，然后将过滤器构件设置为具有高于第一孔隙率的第二孔隙率的非压缩状态，用于回收目标单元。孔隙度的变化是由于过滤构件的孔的直径的扩大或减小。该方法还可以包括当将构件设定为高于第一孔隙度但低于第二孔隙率的孔隙率时，洗涤过滤构件。

2. 发明授权

US5547576A Pathogenic substance removing material and a blood filter containing the material 失效
标题翻译：病原物除去物质和含有该物质的血液过滤器
公开(公告)号：US5547576A
公开(公告)日：1996-08-20
申请号：US85813
申请日：1993-07-06
申请人： Makoto Onishi , Takashi Ohwada , Ken Tatebe , Yoshitaka Ohmura , Kenichi Shimura
发明人： Makoto Onishi , Takashi Ohwada , Ken Tatebe , Yoshitaka Ohmura , Kenichi Shimura
IPC分类号： A61L2/00 , A61L2/02 , A61M1/36 , B01D39/16 , B01D67/00 , B01D63/00 , B01D69/12 , B01D71/60
CPC分类号： B01D67/0093 , A61L2/0017 , A61L2/022 , A61M1/3633 , B01D39/1623 , A61M2202/0415 , B01D2323/38
摘要： A material wherein a polyamine compound is immobilized on its base surface and a blood filter using this material. The material is provided in the form of a porous membrane having the maximum pore diameter of 0.1-50 .mu.m. The polyamine compound is at least one compound selected from the group consisting of (a) a polyamine compound which has primary and secondary amines in its molecule and a hydrophobic part between these amines, (b) a polyamine compound represented by the following formula (I); ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5 represent hydrogen, aliphatic and aromatic hydrocarbons, and halogen, and n is 5 or more, and (c) a polyamine compound represented by the following formula (II); ##STR2## wherein R represents NH.sub.2 or aminoalkyl radical having 1 to 4 carbon atoms and n is 5 or more. The blood filter comprises a housing which has an inlet and outlet, and the material arranged inside the housing.
摘要翻译：其中多胺化合物固定在其基底表面上的材料和使用该材料的血液过滤器。该材料以最大孔径为0.1-50μm的多孔膜的形式提供。多胺化合物是选自（a）在其分子中具有伯胺和仲胺的多胺化合物和这些胺之间的疏水部分的至少一种化合物，（b）由下式（I）表示的多胺化合物）;存在氢，脂族和芳族烃和卤素，n为5以上，（c）由下式（II）表示的多胺化合物。（II）其中R表示NH 2或具有1至4个碳原子且n为5或更大的氨基烷基。血液过滤器包括具有入口和出口的壳体，以及布置在壳体内部的材料。

3. 发明授权

US06312950B1 Apparatus and method for isolating and recovering cells 有权
标题翻译：用于分离和回收细胞的装置和方法
公开(公告)号：US06312950B1
公开(公告)日：2001-11-06
申请号：US09395740
申请日：1999-09-14
申请人： Yoshitaka Ohmura , Miyuki Shimizu , Tadashi Sameshima
发明人： Yoshitaka Ohmura , Miyuki Shimizu , Tadashi Sameshima
IPC分类号： C12N500
CPC分类号： B01D69/12 , A61M1/0218 , A61M1/0236 , A61M1/3633 , A61M1/3643 , A61M1/3644 , A61M2202/0439 , A61M2205/7554 , B01D61/14 , B01D69/02 , A61M2202/005
摘要： There is proposed an apparatus for isolating and recovering an objective cell, which comprises, a filtering device having a filtering member which is capable of setting a porosity thereof to two or more different porosity, a first line for feeding a priming liquid to the filtering device, a second line for feeding a liquid to be treated containing the cell aimed to isolate to the filtering device, and a third line provided with a storage container for storing the priming liquid that has been passed through the filtering device, wherein the priming liquid stored in the storage container is employed for washing the filtering device. There is also proposed a method for isolating and recovering an objective cell using the aforementioned apparatus.
摘要翻译：提出了一种用于分离和回收目标单元的装置，其包括：过滤装置，其具有能够将其孔隙率设定为两个或多个不同孔隙率的过滤构件，用于将起动液体供给到过滤装置的第一管线用于供给待处理的液体的第二管线，其中所述待处理液体旨在与所述过滤装置隔离;以及第三管线，设置有用于储存已经通过所述过滤装置的引发液体的储存容器，其中所述引发液体存储在储存容器中用于洗涤过滤装置。还提出了使用上述装置分离和回收目标单元的方法。

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