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1. 发明授权

US06955879B2 Method for generating recombinant DNA library using unidirectional single-stranded DNA fragments 有权
标题翻译：使用单向单链DNA片段生成重组DNA文库的方法
公开(公告)号：US06955879B2
公开(公告)日：2005-10-18
申请号：US10148724
申请日：2001-06-16
申请人： Si-Hyoung Lee , Yong-Chul Shin , Yeong-Joong Jeon , Kyung-Hwa Jung , Eun-Jung Ryu , Ko-Woon Lee
发明人： Si-Hyoung Lee , Yong-Chul Shin , Yeong-Joong Jeon , Kyung-Hwa Jung , Eun-Jung Ryu , Ko-Woon Lee
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C12P19/34 , C12P21/06
CPC分类号： C12N15/1027
摘要： The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones. The method of the present invention can be used for in vitro recombination of homologous polynucleotides and the directed molecular evolution.
摘要翻译：本发明涉及一种生产重组多核苷酸的方法，包括以下步骤：从两个或多个同源双链多核苷酸产生单向单链多核苷酸片段池，使用单向单链多核苷酸进行包含多循环延伸反应的聚合方法标记的多核苷酸片段作为模板和特异性寡核苷酸作为引物以获得重组多核苷酸，并使用至少一个引物进行聚合酶链反应以扩增重组多核苷酸; 以及构建重组DNA文库的方法，包括以下步骤：将通过上述方法制备的重组多核苷酸插入载体中，并用含有重组多核苷酸的载体转化表达细胞以获得多个突变克隆。本发明的方法可用于同源多核苷酸的体外重组和定向分子进化。

2. 发明申请

US20140199738A1 METHOD FOR PREPARING VOLATILE FATTY ACIDS FROM THE PRE-TREATED EXTRACTS OF MARINE BIOMASS RESIDUE 审中-公开
标题翻译：从海洋生物质残留物的预处理提取物中制备挥发性脂肪酸的方法
公开(公告)号：US20140199738A1
公开(公告)日：2014-07-17
申请号：US13807587
申请日：2011-12-16
申请人： Hee-Chul Woo , Ho-Nam Chang , Yeong-Joong Jeon , Dong-Jin Suh , Byung-Soo Chun , Kyeong-Keun Oh , Kyoung-Heon Kim , Du-Woon Kim , Jae-Hyung Choi
发明人： Hee-Chul Woo , Ho-Nam Chang , Yeong-Joong Jeon , Dong-Jin Suh , Byung-Soo Chun , Kyeong-Keun Oh , Kyoung-Heon Kim , Du-Woon Kim , Jae-Hyung Choi
IPC分类号： C12P7/64
CPC分类号： C12P7/6409 , C12N1/20 , C12P7/40 , C12P7/52 , C12P7/54 , Y02P20/544
摘要： A method of preparing a volatile fatty acids (VFAs) is provided. More particularly, the method includes chemically or biologically pretreating a residue of algae to obtain an extract of the algae residue, filtering the extract of the algae residue and anaerobically fermenting the filtrate. The method of preparing VFAs can be used regardless of the kinds of algae, a process of producing VFAs can be simplified since there is no need to develop an enzyme for anaerobic fermentation, and a yield of VFAs can be increased since the VFAs can be produced from different kinds of organic components such as proteins or fats in addition to carbohydrates. Accordingly, the method can be useful in producing VFAs in an economical and efficient manner.
摘要翻译：提供了制备挥发性脂肪酸（VFA）的方法。更具体地，该方法包括化学或生物地预处理藻类的残余物以获得藻类残渣的提取物，过滤藻类残渣的提取物并厌氧发酵滤液。可以使用制备VFA的方法，而不管藻类的种类如何，由于不需要开发用于厌氧发酵的酶，因此可以简化生产VFA的过程，并且可以增加VFAs的产率，因为可以产生VFA 来自不同种类的有机组分如蛋白质或脂肪以及碳水化合物。因此，该方法可有效地以经济和有效的方式生产VFA。

3. 发明授权

US06800474B1 Trehalose synthase protein, gene, plasmids, microorganisms, and a process for producing trehalose 失效
标题翻译：海藻糖合成酶蛋白，基因，质粒，微生物和生产海藻糖的方法
公开(公告)号：US06800474B1
公开(公告)日：2004-10-05
申请号：US09937215
申请日：2001-09-24
申请人： Se Yong Lee , Eun Kyung Song , Yearn Hung Park , Sang Ho Kwon , Kwang Ho Lee , Chang Gyeom Kim , Jin Ho Lee , Sung Oh Chung , Yeong Joong Jeon
发明人： Se Yong Lee , Eun Kyung Song , Yearn Hung Park , Sang Ho Kwon , Kwang Ho Lee , Chang Gyeom Kim , Jin Ho Lee , Sung Oh Chung , Yeong Joong Jeon
IPC分类号： C12N990
CPC分类号： C12R1/38 , C12N9/90 , C12P19/24
摘要： The present invention relates to a trehalose-producing microorganism and a process for producing trehalose. It also to a novel trehalose synthase protein, a trehalose synthase gene, recombinant plasmids carrying said trehalose synthase gene, and transformed microorganism with said recombinant plasmids.
摘要翻译：本发明涉及产生海藻糖的微生物和生产海藻糖的方法。还有一种新型海藻糖合成酶蛋白，海藻糖合成酶基因，携带所述海藻糖合成酶基因的重组质粒和用所述重组质粒转化的微生物。

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