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    • 2. 发明授权
    • Method of producing fractions containing a high concentration of milk basic cystatin and decomposition products thereof
    • 制备含有高浓度的乳碱性半胱氨酸蛋白酶抑制剂及其分解产物的级分的方法
    • US06649590B2
    • 2003-11-18
    • US09876267
    • 2001-06-06
    • Yukihiro TakadaAtsushi SerizawaYasuhiro MatsuokaYasuhiro TobaHiroshi Kawakami
    • Yukihiro TakadaAtsushi SerizawaYasuhiro MatsuokaYasuhiro TobaHiroshi Kawakami
    • A61K3800
    • A61K35/20A21D2/263A21D13/80A23C9/1307A23C19/082A23J1/20A23J3/08A23J3/343A23K20/147A23K20/174A23K20/22A23L33/19
    • A milk basic protein fraction is heated if necessary, the resulting precipitate is removed to recover the resulting supernatant, alcohol is added to this supernatant, a fraction containing a high concentration of milk basic cystatin is recovered by removing the resulting precipitate and brought into contact with a carrier containing a sulfuric acid group, such as heparin, to recover a fraction which is not adsorbed onto the carrier, and this fraction is then treated with an ultrafiltration membrane having an cut-off molecular weight of 10-50 kDa to recover the permeate to produce a fraction containing a high concentration of milk basic cystatin. Alternatively, a milk-derived basic protein composition is brought into contact with an anion exchange resin to recover a fraction which is not adsorbed onto the resin, after which this fraction is brought into contact with a cation exchange resin to recover the target fraction by eluting a fraction which is adsorbed onto the resin with an eluent to produce a fraction containing a high concentration of milk basic cystatin. Further, a decomposition product of the fraction containing a high concentration of milk basic cystatin is produced by decomposing this fraction containing a high concentration of milk basic cystatin with protease.
    • 如果需要,加热牛奶碱性蛋白质级分,除去所得沉淀物以回收所得上清液,向该上清液中加入醇,通过除去所得沉淀物并与之接触,回收含有高浓度的乳碱性半胱氨酸蛋白酶的级分 含有硫酸基的载体如肝素,以回收未吸附到载体上的级分,然后用截留分子量为10-50kDa的超滤膜处理该级分以回收渗透物 以产生含有高浓度的牛奶碱性半胱氨酸蛋白酶的部分。 或者,将乳衍生的碱性蛋白质组合物与阴离子交换树脂接触以回收未吸附到树脂上的级分,之后使该级分与阳离子交换树脂接触,以通过洗脱来回收目标级分 用洗脱液吸附到树脂上的一部分,以产生含有高浓度的乳碱性半胱氨酸蛋白酶的部分。 此外,含有高浓度的乳碱性半胱氨酸蛋白酶抑制剂的级分的分解产物是通过将含有高浓度的乳碱性半胱氨酸蛋白酶的部分用蛋白酶分解而产生的。
    • 3. 发明申请
    • STREPTAVIDIN-COUPLED MAGNETIC PARTICLES AND MANUFACTURING METHOD FOR SAME
    • STREPTAVIDIN-偶联磁性颗粒及其制造方法
    • US20140051070A1
    • 2014-02-20
    • US13985521
    • 2012-02-15
    • Nobuyuki AraiYasuhiro MatsuokaKazuki Morita
    • Nobuyuki AraiYasuhiro MatsuokaKazuki Morita
    • G01N33/566
    • G01N33/566G01N33/531G01N33/54326G01N33/5434
    • The present invention provides a streptavidin-coupled magnetic particle with high biotin-binding capacity, and a manufacturing method thereof. The streptavidin-coupled magnetic particle has a structure in which streptavidins are cross-linked with each other on a magnetic particle. A method for manufacturing the streptavidin-coupled magnetic particle includes the steps of:(1) preparing a suspension containing magnetic particles having amino groups on their surface; and (2) reacting the magnetic particles with streptavidin and glutaraldehyde by adding glutaraldehyde in the presence of streptavidin to the suspension prepared in step (1). The streptavidin-coupled magnetic particle of the present invention, and the streptavidin-coupled magnetic particle manufactured by the manufacturing method of the present invention are useful in clinical diagnosis.
    • 本发明提供具有高生物素结合能力的链霉抗生物素蛋白偶联磁性颗粒及其制造方法。 链霉抗生物素蛋白偶联磁性颗粒具有链霉亲和素在磁性颗粒上彼此交联的结构。 制备链霉抗生物素蛋白偶联磁性颗粒的方法包括以下步骤:(1)制备含有表面具有氨基的磁性颗粒的悬浮液; 和(2)通过在链霉亲和素存在下将戊二醛加入到步骤(1)中制备的悬浮液中使磁性颗粒与链霉亲和素和戊二醛反应。 本发明的链霉抗生物素蛋白偶联磁性颗粒和通过本发明的制造方法制造的链霉抗生物素蛋白偶联的磁性颗粒可用于临床诊断。
    • 6. 发明申请
    • Display Panel Sealing Clip
    • 显示面板密封夹
    • US20080099153A1
    • 2008-05-01
    • US11722804
    • 2005-09-30
    • Akihiro FujimotoYasuhiro Matsuoka
    • Akihiro FujimotoYasuhiro Matsuoka
    • B29C65/48
    • H01J9/261
    • An attitude-changeable surface pressing member is attached to a front end of a clip such that substrates can evenly be pressed even if a distance clipped at the front end by the clip is changed during a heating process in a sealing process. A display panel sealing clip includes a pressing member which has a pair of clipping portions for clipping both the substrates and the tubulation tubing to apply pressure in a direction in which the pair of clipping portions is brought close to each other and a surface pressing member which presses the tubulation tubing by a surface thereof. The surface pressing member has a notch portion which surrounds a cylindrical portion of the tubulation tubing from three directions, and the surface pressing member is attached to one of the clipping portions of the pressing member in an attitude-changeable manner such that the flange portion is pressed by the surface of the surface pressing member even if a distance between the pair of clipping portions of the pressing member is changed after the tubulation tubing is tentatively fixed.
    • 即使在密封过程中的加热过程中,即使在夹子的前端夹住的距离发生变化,也可以将夹具的前端部安装在姿势可变的表面按压部件上。 显示面板密封夹包括按压构件,该按压构件具有用于夹持基板和管状管的一对夹持部,以在一对夹持部彼此靠近的方向上施加压力;以及表面按压构件, 通过其表面挤压管状管。 表面按压部件具有从三个方向围绕管状管的圆筒部的切口部,并且,表面按压部件以能够改变姿态的方式安装在按压部件的一个夹持部上,使得凸缘部为 即使在短暂地固定管状管之后,按压构件的一对夹持部之间的距离改变,也被表面按压构件的表面按压。