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1. 发明授权

US07781645B2 Expression system for synthetic zinc finger proteins and methods of using the same 有权
标题翻译：合成锌指蛋白的表达系统及其使用方法
公开(公告)号：US07781645B2
公开(公告)日：2010-08-24
申请号：US12127763
申请日：2008-05-27
申请人： Carlos F. Barbas, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
发明人： Carlos F. Barbas, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8261 , Y02A40/146
摘要： The invention relates to the field of plant and agricultural technology. More specifically, the invention relates to the use of zinc finger proteins and fusions of said proteins to regulate gene expression and metabolic pathways in plants.
摘要翻译：本发明涉及植物和农业技术领域。更具体地，本发明涉及锌指蛋白和所述蛋白质的融合物在植物中调节基因表达和代谢途径的用途。

2. 发明授权

US07807868B2 Mobilization of viral genomes from T-DNA using site-specific recombination systems 有权
标题翻译：使用位点特异性重组系统动员来自T-DNA的病毒基因组
公开(公告)号：US07807868B2
公开(公告)日：2010-10-05
申请号：US12061315
申请日：2008-04-02
申请人： Christopher L. Baszczynski , Lesek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
发明人： Christopher L. Baszczynski , Lesek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
IPC分类号： C12N15/82 , C12N15/90 , C12N5/10
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.
摘要翻译：本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。感兴趣的DNA还可以含有重组酶的不相同的靶位点。用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。位点特异性重组酶在植物细胞中的表达导致切除病毒复制子和感兴趣的相关DNA。然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。

3. 发明申请

US20080282426A1 MOBILIZATION OF VIRAL GENOMES FROM T-DNA USING SITE-SPECIFIC RECOMBINATION SYSTEMS 有权
标题翻译：使用现场特异性重组系统从T-DNA中动员病毒基因
公开(公告)号：US20080282426A1
公开(公告)日：2008-11-13
申请号：US12061315
申请日：2008-04-02
申请人： Christopher L. Baszczynski , Lesek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
发明人： Christopher L. Baszczynski , Lesek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.
摘要翻译：本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。感兴趣的DNA还可以含有重组酶的不相同的靶位点。用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。位点特异性重组酶在植物细胞中的表达导致病毒复制子和感兴趣的相关DNA的切除。然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。本发明的组合物包含核酸，例如含有侧翼为用于位点特异性重组酶的直接重复靶位点的病毒DNA的T-DNA。本发明的核酸可以另外含有编码针对病毒基因组侧翼的靶位点的同源位点特异性重组酶的表达盒。本发明的组合物还包含含有本发明核酸的农杆菌。本发明的组合物和方法用于增加农业感染的效率，为临时表达提供高拷贝数的目的DNA，或用于整合到植物染色体中，以及简化用于农业感染和转化的载体的构建和稳定维持。

4. 发明授权

US07378510B2 Synthetic zinc finger protein encoding sequences and methods of producing the same 失效
标题翻译：合成锌指蛋白编码序列及其制备方法
公开(公告)号：US07378510B2
公开(公告)日：2008-05-27
申请号：US11639706
申请日：2006-12-14
申请人： Carlos F. Barbas, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
发明人： Carlos F. Barbas, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8261 , Y02A40/146
摘要： The invention relates to nucleic acids encoding synthetic zinc finger proteins for gene regulation in plants. The invention also relates to methods of producing synthetic zinc finger protein in host cells.
摘要翻译：本发明涉及编码植物中基因调控的合成锌指蛋白的核酸。本发明还涉及在宿主细胞中产生合成锌指蛋白的方法。

5. 发明授权

US06262341B1 Method for the integration of foreign DNA into eukaryotic genomes 有权
标题翻译：将外源DNA整合到真核生物基因组中的方法
公开(公告)号：US06262341B1
公开(公告)日：2001-07-17
申请号：US09193503
申请日：1998-11-17
申请人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan , Argula Gururaj Rao , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan , Argula Gururaj Rao , Laura A. Tagliani
IPC分类号： C12N504
CPC分类号： C12N15/90 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/8213
摘要： Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells. The methods result in the efficient targeted genomic integration of DNA by site-specific recombination.
摘要翻译：提供了将目的DNA引入基因组靶位点的组合物和方法。特别地，所述方法和组合物包括使用两个位点特异性重组酶的靶位点的组合和具有双靶点位点特异性的嵌合重组酶的表达。因此，组合物包含对多个靶位点具有特异性的新的位点特异性重组酶，以及编码这些重组酶或靶位点的核苷酸序列和表达盒。所述方法包括用具有相应靶位点侧翼的感兴趣的DNA转化具有新重组酶靶位点的真核细胞。重组酶的表达导致感兴趣的DNA整合到细胞的基因组中。本发明的组合物和方法用于构建稳定转化的真核细胞，特别是植物细胞。该方法通过位点特异性重组导致DNA的有效靶向基因组整合。

6. 发明申请

US20090007300A1 SYNTHETIC ZINC FINGER PROTEIN ENCODING SEQUENCES AND METHODS OF PRODUCING THE SAME 有权
标题翻译：合成锌指蛋白编码序列及其生产方法
公开(公告)号：US20090007300A1
公开(公告)日：2009-01-01
申请号：US12127763
申请日：2008-05-27
申请人： Carlos F. BARBAS, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
发明人： Carlos F. BARBAS, III , Justin T. Stege , Xueni Guan , Bipin Dalmia
IPC分类号： A01H5/00 , C12N5/04 , C12N15/82 , C12N15/00
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8261 , Y02A40/146
摘要： The invention relates to the field of plant and agricultural technology. More specifically, the invention relates to the use of zinc finger proteins and fusions of said proteins to regulate gene expression and metabolic pathways in plants.
摘要翻译：本发明涉及植物和农业技术领域。更具体地，本发明涉及锌指蛋白和所述蛋白质的融合物在植物中调节基因表达和代谢途径的用途。

7. 发明申请

US20060253939A1 Mobilization of viral genomes from T-DNA using site-specific recombination systems 失效
公开(公告)号：US20060253939A1
公开(公告)日：2006-11-09
申请号：US11400293
申请日：2006-04-07
申请人： Christopher Baszczynski , Leszek Lyznik , William Gordon-Kamm , Xueni Guan
发明人： Christopher Baszczynski , Leszek Lyznik , William Gordon-Kamm , Xueni Guan
IPC分类号： A01H1/00 , C12N15/82 , C12N1/20
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.

8. 发明授权

US06541231B1 Method for the integration of foreign DNA into eukaryotic genomes 有权
标题翻译：将外源DNA整合到真核生物基因组中的方法
公开(公告)号：US06541231B1
公开(公告)日：2003-04-01
申请号：US09415839
申请日：1999-10-12
申请人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan , Aragula Gururaj Rao , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan , Aragula Gururaj Rao , Laura A. Tagliani
IPC分类号： C12N900
CPC分类号： C12N15/90 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/8213
摘要： Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells. The methods result in the efficient targeted genomic integration of DNA by site-specific recombination.
摘要翻译：提供了将目的DNA引入基因组靶位点的组合物和方法。特别地，所述方法和组合物包括使用两个位点特异性重组酶的靶位点的组合和具有双靶点位点特异性的嵌合重组酶的表达。因此，组合物包含对多个靶位点具有特异性的新的位点特异性重组酶，以及编码这些重组酶或靶位点的核苷酸序列和表达盒。所述方法包括用具有相应靶位点侧翼的感兴趣的DNA转化具有新重组酶靶位点的真核细胞。重组酶的表达导致感兴趣的DNA整合到细胞的基因组中。本发明的组合物和方法用于构建稳定转化的真核细胞，特别是植物细胞。该方法通过位点特异性重组导致DNA的有效靶向基因组整合。

9. 发明授权

US06300545B1 Mobilization of viral genomes from T-DNA using site-specific recombination systems 有权
标题翻译：使用位点特异性重组系统动员来自T-DNA的病毒基因组
公开(公告)号：US06300545B1
公开(公告)日：2001-10-09
申请号：US09193475
申请日：1998-11-17
申请人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
发明人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
IPC分类号： C12N504
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons from T-DNA. The methods comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.
摘要翻译：本发明涉及用于来自T-DNA的病毒复制子的位点特异性重组酶介导的动员的方法和组合物。所述方法包括农杆菌介导的T-DNA向植物细胞的转移，其中所述T-DNA含有侧翼于病毒复制子的病毒复制子，所述病毒复制子侧接于位点特异性重组酶和任选的与所述病毒复制子连接的感兴趣的DNA。感兴趣的DNA还可以含有重组酶的不相同的靶位点。用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。位点特异性重组酶在植物细胞中的表达导致病毒复制子和感兴趣的相关DNA的切除。然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。本发明的组合物包含核酸，例如含有侧翼为用于位点特异性重组酶的直接重复靶位点的病毒DNA的T-DNA。本发明的核酸可以另外含有编码针对病毒基因组侧翼的靶位点的同源位点特异性重组酶的表达盒。本发明的组合物还包含含有本发明核酸的农杆菌。本发明的组合物和方法用于增加农业感染的效率，为临时表达提供高拷贝数的目的DNA，或用于整合到植物染色体中，以及简化用于农业感染和转化的载体的构建和稳定维持。

10. 发明授权

US07364902B2 Mobilization of viral genomes from T-DNA using site-specific recombination systems 失效
标题翻译：使用位点特异性重组系统动员来自T-DNA的病毒基因组
公开(公告)号：US07364902B2
公开(公告)日：2008-04-29
申请号：US11400293
申请日：2006-04-07
申请人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
发明人： Christopher L. Baszczynski , Leszek Alexander Lyznik , William J. Gordon-Kamm , Xueni Guan
IPC分类号： C12N15/82 , C12N5/04 , C12N5/10 , A01H1/00 , C07H21/00 , C07H21/02
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention.
摘要翻译：本发明涉及用于位点特异性重组酶介导的来自T-DNA的感兴趣的病毒复制子和相关DNA的动员的方法和组合物。本发明的方法包括农杆菌介导的T-DNA向植物细胞的转移，其中T-DNA含有病毒复制子，其侧翼是用于位点特异性重组酶的直接重复的靶位点，以及任选的与病毒连接的感兴趣的DNA 复制品。感兴趣的DNA还可以含有重组酶的不相同的靶位点。用于位点特异性重组酶的表达盒存在于T-DNA或植物基因组上，或瞬时引入植物细胞。位点特异性重组酶在植物细胞中的表达导致病毒复制子和感兴趣的相关DNA的切除。然后将病毒复制子和感兴趣的DNA复制到宿主植物细胞中的高拷贝数。本发明的组合物包含核酸，例如含有侧翼为用于位点特异性重组酶的直接重复靶位点的病毒DNA的T-DNA。本发明的核酸可以另外含有编码针对病毒基因组侧翼的靶位点的同源位点特异性重组酶的表达盒。本发明的组合物还包含含有本发明核酸的农杆菌。

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