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1. 发明申请

US20130295663A1 Pressurizable Cartridge for Polymerase Chain Reactions 有权
标题翻译：可压缩胶囊用于聚合酶链反应
公开(公告)号：US20130295663A1
公开(公告)日：2013-11-07
申请号：US13813882
申请日：2011-08-01
申请人： Brent L. Weight , William D. Bickmore , Darryl K. Zitting
发明人： Brent L. Weight , William D. Bickmore , Darryl K. Zitting
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/5082 , B01L7/52 , B01L2200/0684 , B01L2300/022 , B01L2300/0681 , B01L2300/123 , B01L2400/0478
摘要： Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample.
摘要翻译：提供了与聚合酶链反应的性能有关的方法和装置。描述了包括样品组件和PCR组件的示例性样品处理模块，样品处理模块被配置为在高于环境压力的压力下将样品保持在其中。在使用时将样品添加到样品组件中，然后将其连接到PCR组件。药筒的实施方案包括能够或有助于在反应小瓶内产生较高压力的流量限制装置。将样品引入PCR反应小瓶中，其包含处理样品所需的PCR反应混合物的所有成分，并且如果DNA存在于样品中，则提供所需的扩增的DNA。

2. 发明授权

US07315376B2 Fluorescence detection system 有权
标题翻译：荧光检测系统
公开(公告)号：US07315376B2
公开(公告)日：2008-01-01
申请号：US11031526
申请日：2005-01-07
申请人： William D. Bickmore, Jr. , Danvern Ray Roberts
发明人： William D. Bickmore, Jr. , Danvern Ray Roberts
IPC分类号： G01N21/25
CPC分类号： G01N21/6452
摘要： Apparatus and methods for exciting and detecting fluorescence in samples are disclosed. In one embodiment, a sample holder for holding a plurality of samples is provided together with an optical manifold having an excitation source, a photo receiver, or both, for each of the plurality of samples. In another embodiment, the optical manifold contains only the excitation source or a photo receiver, and the other is associated with the sample holder. This system permits for rapid excitation and measurement of fluorescence without the use of moving parts and without any opto-mechanical or electronic disturbance. It exhibits an exceptional signal to noise ratio, which permits it to differentiate between very low level differences in fluorescence.
摘要翻译：公开了用于激发和检测样品中的荧光的装置和方法。在一个实施例中，用于保持多个样品的样品保持器与具有用于多个样品中的每一个的激发源，光接收器或两者的光学歧管一起提供。在另一个实施例中，光学歧管仅包含激发源或光电接收器，另一个与样品架相关联。该系统允许快速激发和测量荧光，而不使用移动部件，没有任何光机械或电子干扰。它具有出色的信噪比，可以区分出非常低的荧光水平差异。

3. 发明公开

US20230323441A1 Rapid Simplified PCR Point-of-Care Cartridge System with Highly Sensitive Fluorescent Reading Optics Which Starts Reading at the First Thermal Cycle. 审中-公开
公开(公告)号：US20230323441A1
公开(公告)日：2023-10-12
申请号：US17715705
申请日：2022-04-07
申请人： William D. Bickmore , Neil D. Holland
发明人： William D. Bickmore , Neil D. Holland
IPC分类号： C12Q1/686 , B01L7/00 , B01L3/00
CPC分类号： C12Q1/686 , B01L7/52 , B01L2400/0481 , B01L2300/1827 , B01L2400/0478 , B01L3/50273
摘要： A single-use special-purpose disposable vertically operated Polymerase Chain Reaction (PCR) cartridge that automatically performs extraction of Deoxyribonucleic Acid (DNA) or Ribonucleic Acid (RNA) using focused ultrasound and its associated transient cavitation pressure followed by automatic purification and distribution to a plurality of PCR thermocycling vials. The cartridge utilizes a novel optical system that allows reading of the PCR binary expansion of Double-Stranded DNA (dsDNA) at early cycles usually considered unreadable background noises resulting in not only exceptionally fast sample preparation but very quick data confirming positive or negative samples in as little as 10 cycles. The PCR vials contain lyophilized, pellets comprising all the necessary chemicals for PCR. This process requires no ancillary equipment such as microfuges, or pipettes. Once the DNA or RNA containing sample and the processing fluid are added to the cartridge, all else is automatic. The process fluid passes through each part of the cartridge only once. There are no revolving mechanical parts or otherwise complex mechanical systems. The cartridge does not vent into the atmosphere and minimizes environmental contamination. The cartridge is tightly sealed to prevent leakage.

4. 发明申请

US20120186651A1 APPARATUS, SYSTEMS AND METHODS FOR COLLECTING SOLAR ENERGY 审中-公开
标题翻译：收集太阳能的装置，系统和方法
公开(公告)号：US20120186651A1
公开(公告)日：2012-07-26
申请号：US13357398
申请日：2012-01-24
申请人： William D. Bickmore
发明人： William D. Bickmore
IPC分类号： H01L31/0232 , H01L31/18
CPC分类号： H01L31/02168 , H01L31/0543 , Y02B10/10 , Y02E10/52
摘要： Apparatuses and systems for collecting solar energy may include a plurality of holographic optical elements (HOEs) associated with a photovoltaic (PV) cell. Material layers containing HOEs may be attached to directly to a surface of a PV cell or attached to the surface of the module that houses a PV cell. The HOEs alter the angle of incoming sunlight such that the angle of incidence (AOI) of the sunlight on the PV cell is within an accepted angle of acceptance (AOA). In one embodiment one or more material layers have HOEs formed therein and are arranged with respect to the photovoltaic cell such that the plurality of HOEs redirect sunlight along at least one axis when the sunlight is outside a defined AOA and permit sunlight to pass through the at least one material layer without being redirected when the sunlight is within the defined AOA.
摘要翻译：用于收集太阳能的装置和系统可以包括与光伏（PV）电池相关联的多个全息光学元件（HOE）。包含HOEs的材料层可以直接连接到PV电池的表面或附着到容纳PV电池的模块的表面。 HOE改变入射的太阳光的角度，使得PV电池上的太阳光的入射角（AOI）在接受的接受角（AOA）内。在一个实施例中，一个或多个材料层具有在其中形成的HOE并且相对于光伏电池布置，使得当太阳光在限定的AOA之外时，多个HOE沿着至少一个轴重定向太阳光，并允许太阳光通过当太阳光在定义的AOA内时，不会重新定向至少一个材料层。

5. 发明授权

US08169122B1 Ultra sonic release of DNA or RNA 有权
标题翻译：超声释放DNA或RNA
公开(公告)号：US08169122B1
公开(公告)日：2012-05-01
申请号：US13243868
申请日：2011-09-23
申请人： Danvern R. Roberts , William D. Bickmore , Jared Hummel , Daniel Esplin , Paul Day
发明人： Danvern R. Roberts , William D. Bickmore , Jared Hummel , Daniel Esplin , Paul Day
IPC分类号： H01L41/08
CPC分类号： C12N13/00
摘要： Method and apparatus for sonication in connection with preparing a cellular sample containing DNA or RNA for performance of polymerase chain reaction (PCR) or for other reasons where it is important to break down the cell walls and other cellular structures to release cellular contents including DNA. The same methods and apparatus may be used to release DNA or RNA from virus for PCR or other uses. A novel apparatus which is capable of releasing cellular contents or releasing the DNA or RNA of virus without the aid of beads or chemicals is described herein. The apparatus is designed to deliver high levels of sonic energy through optimizing the geometry the apparatus and optimizing the force created by a piezoelectric transducer. The apparatus is capable of processing samples which are contained within fluid in a short amount of time between 30 seconds and two and one half minutes. The apparatus is small and can be field deployable or used in a standard molecular biology laboratory.
摘要翻译：与制备含有DNA或RNA的细胞样品进行聚合酶链反应（PCR）或其他原因的超声处理方法和装置，其中重要的是分解细胞壁和其他细胞结构以释放包括DNA的细胞内容物。可以使用相同的方法和装置从病毒中释放DNA或RNA用于PCR或其他用途。本文描述了能够释放细胞内容物或释放病毒DNA或RNA的新颖设备，无需借助于珠粒或化学物质。该设备旨在通过优化设备的几何形状和优化由压电换能器产生的力来提供高水平的声能。该装置能够在30秒至2.5分钟之间的短时间内处理包含在流体内的样品。该设备很小，可以在现场部署或用于标准分子生物学实验室。

6. 发明授权

US07993910B2 Methods and apparatus for amplification of DNA using sonic energy 有权
标题翻译：使用声能扩增DNA的方法和装置
公开(公告)号：US07993910B2
公开(公告)日：2011-08-09
申请号：US12372904
申请日：2009-02-18
申请人： William D. Bickmore , Danvern Ray Roberts
发明人： William D. Bickmore , Danvern Ray Roberts
IPC分类号： C12M1/34
CPC分类号： G01N21/6428 , B01L7/52 , B01L99/00 , B01L2300/18 , B01L2400/0439 , C12Q1/6844 , G01N21/6408 , C12Q2563/107 , C12Q2561/113 , C12Q2523/301
摘要： Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
摘要翻译：提供用于扩增DNA的装置和方法，其使用声能代替常规热循环仪。在一个实施方案中，将声能量施加到PCR混合物以使双链DNA解离成单链DNA。没有声波能量的静止阶段导致DNA的扩增。重复使用声能的循环和停止应用声能导致DNA扩增。

7. 发明申请

US20090155893A1 Methods and Apparatus for Amplification of DNA Using Sonic Energy 有权
标题翻译：使用声能扩增DNA的方法和装置
公开(公告)号：US20090155893A1
公开(公告)日：2009-06-18
申请号：US12372904
申请日：2009-02-18
申请人： William D. Bickmore, JR. , Danvern Ray Roberts
发明人： William D. Bickmore, JR. , Danvern Ray Roberts
IPC分类号： C12M1/40
CPC分类号： G01N21/6428 , B01L7/52 , B01L99/00 , B01L2300/18 , B01L2400/0439 , C12Q1/6844 , G01N21/6408 , C12Q2563/107 , C12Q2561/113 , C12Q2523/301
摘要： Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
摘要翻译：提供用于扩增DNA的装置和方法，其使用声能代替常规热循环仪。在一个实施方案中，将声能量施加到PCR混合物以使双链DNA解离成单链DNA。没有声波能量的静止阶段导致DNA的扩增。重复使用声能的循环和停止应用声能导致DNA扩增。

8. 发明授权

US07504219B2 Methods and apparatus for amplification of DNA using sonic energy 有权
标题翻译：使用声能扩增DNA的方法和装置
公开(公告)号：US07504219B2
公开(公告)日：2009-03-17
申请号：US11432935
申请日：2006-05-12
申请人： William D. Bickmore, Jr. , Danvern Ray Roberts
发明人： William D. Bickmore, Jr. , Danvern Ray Roberts
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N21/6428 , B01L7/52 , B01L99/00 , B01L2300/18 , B01L2400/0439 , C12Q1/6844 , G01N21/6408 , C12Q2563/107 , C12Q2561/113 , C12Q2523/301
摘要： Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
摘要翻译：提供用于扩增DNA的装置和方法，其使用声能代替常规热循环仪。在一个实施方案中，将声能量施加到PCR混合物以使双链DNA解离成单链DNA。没有声波能量的静止阶段导致DNA的扩增。重复使用声能的循环和停止应用声能导致DNA扩增。

9. 发明授权

US09816131B2 Pressurizable cartridge for polymerase chain reactions 有权
公开(公告)号：US09816131B2
公开(公告)日：2017-11-14
申请号：US13813882
申请日：2011-08-01
申请人： Brent L. Weight , William D. Bickmore , Darryl K. Zitting
发明人： Brent L. Weight , William D. Bickmore , Darryl K. Zitting
IPC分类号： B01L1/00 , B01L3/00 , C12Q1/68 , B01L7/00
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/5082 , B01L7/52 , B01L2200/0684 , B01L2300/022 , B01L2300/0681 , B01L2300/123 , B01L2400/0478
摘要： Methods and apparatus for use in connection with the performance of the polymerase chain reaction are provided. An exemplary sample processing module is described that includes a sample assembly and a PCR assembly, the sample processing module being configured to hold the sample therein at a pressure higher than ambient pressure. A sample is added to the sample assembly at the time of use, which is then connected to the PCR assembly. Embodiments of the cartridge include a flow restriction device that enables or aids in creating a higher pressure within the reaction vial. The sample is introduced into a PCR reaction vial, which contains all of the constituents of a PCR reaction mixture that are necessary to process the sample and provide amplified DNA of interest, if that DNA was present in the sample.

10. 发明授权

US07939312B2 Rapid thermocycler with movable cooling assembly 有权
标题翻译：具有可移动冷却组件的快速热循环仪
公开(公告)号：US07939312B2
公开(公告)日：2011-05-10
申请号：US11697917
申请日：2007-04-09
申请人： Danvern R. Roberts , William D. Bickmore, Jr. , Jared S. Hummel
发明人： Danvern R. Roberts , William D. Bickmore, Jr. , Jared S. Hummel
IPC分类号： C12M1/38
CPC分类号： B01L7/52 , B01L2200/147 , B01L2300/0654 , B01L2300/1805 , B01L2300/1894
摘要： Methods and apparatus for effecting rapid thermocycling in connection with the polymerase chain reaction are disclosed. A sample assembly having a relatively small thermal mass is heated to desired PCR operating temperatures, and a separate cooling assembly is used to rapidly lower the temperature as required. In one embodiment, a sample assembly with an integrated heating element is isolated from a relatively large thermal mass cold sink when the temperature of a sample is to be raised or maintained, and brought in contact with the cold sink when the temperature is desired to be lowered.
摘要翻译：公开了与聚合酶链反应有关的快速热循环的方法和装置。具有相对较小的热质量的样品组件被加热到期望的PCR操作温度，并且使用单独的冷却组件以根据需要快速降低温度。在一个实施例中，当要升高或保持样品的温度时，具有集成加热元件的样品组件与相对较大的热质量冷却槽隔离，并且当温度希望为降低

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