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1. 发明申请

US20110143373A1 METHOD OF EVALUATING THE INTEGRITY OF THE PLASMA MEMBRANE OF CELLS BY DETECTING GLYCANS FOUND ONLY INTRACELLULARLY 审中-公开
标题翻译：通过检测仅在细胞内发现的糖蛋白来评估细胞等离子体膜的完整性的方法
公开(公告)号：US20110143373A1
公开(公告)日：2011-06-16
申请号：US13054632
申请日：2009-07-16
申请人： Tia Hirvonen , Annika Kotovuori , Virve Pitkanen , Suvi Natunen , Johanna Nystedt , Sari Tiitinen , Leena Valmu , Jari Natunen
发明人： Tia Hirvonen , Annika Kotovuori , Virve Pitkanen , Suvi Natunen , Johanna Nystedt , Sari Tiitinen , Leena Valmu , Jari Natunen
IPC分类号： G01N33/68 , C12N5/071 , C12N5/0775
CPC分类号： G01N33/5308 , G01N33/56966 , G01N2400/12 , G01N2400/38
摘要： The invention describes novel reagents that can be applied for analysis of the quality of human cells. The method evaluates the integrity of the plasma membrane of the cells by detecting novel glyco structures found only intracellularly. The method can be applied, for example, to demonstrate exposure of therapeutic cell preparation to potentially harmful conditions. It can also be used as a quality control tool in methods in which intact cell membrane is essential and it can be applied in separation of damaged cells from non- damaged.
摘要翻译：本发明描述了可用于分析人细胞质量的新型试剂。该方法通过检测仅在细胞内发现的新型糖结构来评价细胞质膜的完整性。该方法可以例如用于证明治疗细胞制剂暴露于潜在有害的条件。它也可以用作质量控制工具，其中完整的细胞膜是必需的，并且可以应用于损坏的细胞与未损坏的分离。

2. 发明授权

US08889371B2 Lipolytic enzyme: uses thereof in the food industry 有权
标题翻译：脂肪分解酶：在食品工业中的应用
公开(公告)号：US08889371B2
公开(公告)日：2014-11-18
申请号：US12697718
申请日：2010-02-01
申请人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12N9/20
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移至一个或多个酰基受体底物，其中该酶可从棒状杆菌属物种获得。

3. 发明授权

US07666618B2 Lipolytic enzyme: uses thereof in the food industry 失效
标题翻译：脂肪分解酶：在食品工业中的应用
公开(公告)号：US07666618B2
公开(公告)日：2010-02-23
申请号：US11623607
申请日：2007-01-16
申请人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12Q1/34 , C12N9/16
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移到一个或多个酰基受体底物，其中该酶可从链霉菌属物种获得。

4. 发明授权

US08535900B2 Lipolytic enzyme uses thereof in the food industry 失效
标题翻译：脂肪分解酶在食品工业中的用途
公开(公告)号：US08535900B2
公开(公告)日：2013-09-17
申请号：US12697693
申请日：2010-02-01
申请人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12Q1/34 , C12N9/20
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移至一个或多个酰基受体底物，其中该酶可从棒状杆菌属物种获得。

5. 发明申请

US20110086131A1 LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY 有权
标题翻译：脂肪酶：在食品工业中使用
公开(公告)号：US20110086131A1
公开(公告)日：2011-04-14
申请号：US12697718
申请日：2010-02-01
申请人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12N9/20 , C12P19/44 , C12P7/64 , C12P21/06 , C12P7/62 , A23C9/12 , A23J3/34 , A21D2/08 , A23L1/23
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移至一个或多个酰基受体底物，其中该酶可从棒状杆菌属物种获得。

6. 发明申请

US20110014317A1 LIPOLYTIC ENZYME USES THEREOF IN THE FOOD INDUSTRY 失效
标题翻译：脂肪酶在食品工业中的应用
公开(公告)号：US20110014317A1
公开(公告)日：2011-01-20
申请号：US12697693
申请日：2010-02-01
申请人： Andrei MIASNIKOV , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei MIASNIKOV , Jørn Borch Søe , Jørn Dalgaard Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： A23L3/3463 , C12P19/00 , C11C1/04 , C12N9/14 , A23L1/32 , A21D8/04 , A23G4/12 , A23C9/12 , A23L1/31 , A23L1/23
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein the lipolytic enzyme hydrolyzes a glycolipid or a phospholipid or transfers an acyl group from a glycolipid or phospholipids to an acyl acceptor. The present invention also relates to a lipolytic enzyme that hydrolyzes at least a galactolipid or transfers an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Corynebacterium species.
摘要翻译：本发明包括在各种方法和用途中使用可从以下属中的一种获得的脂肪分解酶：链霉菌，棒状杆菌和Thermobifida，其中所述脂肪分解酶水解糖脂或磷脂或将糖基从糖脂或磷脂转移至酰基受体。本发明还涉及一种脂解酶，其至少水解半乳糖脂或将酰基从半乳糖脂转移至一个或多个酰基受体底物，其中该酶可从棒状杆菌属物种获得。

7. 发明申请

US20080131936A1 LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY 失效
标题翻译：脂肪酶：在食品工业中使用
公开(公告)号：US20080131936A1
公开(公告)日：2008-06-05
申请号：US11623607
申请日：2007-01-16
申请人： Andrei Miasnikov , Jorn Soe , Jorn Mikkelsen , Mira Povelainen , Virve Pitkanen
发明人： Andrei Miasnikov , Jorn Soe , Jorn Mikkelsen , Mira Povelainen , Virve Pitkanen
IPC分类号： C12N9/20 , C07H21/00 , C12P19/00 , C11C1/02
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ ID NOs: 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID NO: 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence show in SEQ ID NO: 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID NO: 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID NO: 3.
摘要翻译：本发明包括在各种方法和用途中可以从以下属性之一的链霉菌，棒状杆菌和热嗜热菌获得的脂肪分解酶的用途，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂酰基受体。优选地，用于这些方法和用途的脂肪分解酶包含如SEQ ID NO：4,5,7,8,12,14或16中任一项所示的氨基酸序列或具有至少70％同一性或包含如SEQ ID NO：3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂肪分解酶，其中所述酶可从链霉菌属物种获得，并且包括含有氨基酸的脂肪分解酶如SEQ ID NO：4所示的酸序列或与其具有至少60％同一性的氨基酸序列。该酶可以由选自以下的核酸编码：a）包含SEQ ID NO：3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID NO：3的核苷酸序列相关的核酸; 和c）包含与SEQ ID NO：3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

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