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1. 发明授权

US5185466A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5185466A
公开(公告)日：1993-02-09
申请号：US688752
申请日：1991-04-22
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447
CPC分类号： C08F20/58 , G01N27/44747
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：其中R 1是H，CH 2 OH或（CHOH）mCH 2 OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

2. 发明授权

US5259943A Apparatus and method for submerged gel electrophoresis 失效
标题翻译：浸没式凝胶电泳装置及方法
公开(公告)号：US5259943A
公开(公告)日：1993-11-09
申请号：US909831
申请日：1992-07-07
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： G01N27/447 , B01D61/00
CPC分类号： G01N27/44704
摘要： Electrophoresis apparatus for conducting electrophoresis in submerged gels, comprises a plurality of compartments including at least one gel compartment and at least one reservoir compartment; a plate as the bottom of a gel compartment, electrodes only in a gel compartment; the electrodes arranged in such a way that during operation the created electric field is confined essentially within a rectangular box, the said rectangular box defined on sides by side walls or barriers, on top by air and on bottom by the plate; means to circulate buffer; and barriers in a gel compartment mounted on the plate in front of buffer circulation openings.
摘要翻译：用于在浸没凝胶中进行电泳的电泳装置包括多个隔室，其包括至少一个凝胶室和至少一个贮存室; 作为凝胶室底部的板，电极仅在凝胶室中; 所述电极以这样的方式布置，使得在操作期间，所产生的电场基本上被限制在矩形盒内，所述矩形盒由侧壁或屏障侧面限定，顶部由空气限定，底部由板限定; 循环缓冲的手段以及在缓冲循环开口前面安装在板上的凝胶室中的阻挡层。

3. 发明授权

US5438092A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5438092A
公开(公告)日：1995-08-01
申请号：US145635
申请日：1993-11-04
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447 , C08L39/00
CPC分类号： G01N27/44747 , C08F20/58
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：其中R 1是H，CH 2 OH或（CHOH）mCH 2 OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

4. 发明授权

US5316912A Polyacrylic hydrazides and their applications as glycoprotein reagents 失效
标题翻译：聚丙烯酰肼及其作为糖蛋白试剂的应用
公开(公告)号：US5316912A
公开(公告)日：1994-05-31
申请号：US448105
申请日：1989-12-13
申请人： Urs Heimgartner , Branko Kozulic , Klaus Mosbach
发明人： Urs Heimgartner , Branko Kozulic , Klaus Mosbach
IPC分类号： C08F8/30 , G01N33/532 , G01N33/68 , G01N33/53 , G01N33/543
CPC分类号： G01N33/532 , C08F8/30 , G01N33/6803 , G01N33/6854 , Y10S436/804
摘要： A method for detecting a glycoprotein using a solid support is disclosed where the glycoprotein is oxidized by periodate, polyacrylic polyhydrazide which is a copolymer having repeating units possessing a hydrazide group and repeating units possessing hydroxyl groups is coupled to the oxidized glycoprotein and a glycoenzyme or radioactive compound containing aldehyde groups or activated ketone groups is coupled to the polyacrylic polyhydrazide which allows for detection of the glycoprotein. The glycoprotein may be directly attached to the solid support or may be bound to an antigen which is immobilized on the solid support.
摘要翻译：公开了一种使用固体支持物检测糖蛋白的方法，其中糖蛋白被高碘酸酯聚丙烯酰胺酰胺氧化，其是具有重复单元的具有酰肼基团的共聚物，并且具有羟基的重复单元与氧化糖蛋白和糖酵解或放射性含有醛基或活化酮基的化合物与能够检测糖蛋白的聚丙烯酸聚酰肼偶联。糖蛋白可以直接连接到固体支持物上，或者可以结合固定在固体支持物上的抗原。

5. 发明授权

US5278270A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5278270A
公开(公告)日：1994-01-11
申请号：US972343
申请日：1992-11-06
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447 , C07C233/20
CPC分类号： G01N27/44747 , C08F20/58
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：（*化学结构*）其中R1是H，CH2OH或（CHOH）mCH2OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

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