专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5541255A Cross-linked linear polysaccharide polymers as gels for electrophoresis 失效
标题翻译：交联线性多糖聚合物作为电泳凝胶
公开(公告)号：US5541255A
公开(公告)日：1996-07-30
申请号：US268436
申请日：1994-06-30
申请人： Branko Kozulic
发明人： Branko Kozulic
IPC分类号： B01D57/02 , C07K1/26 , C08B15/00 , C08B31/00 , C08B37/00 , C08B37/12 , C08J3/24 , G01N27/447 , C08L5/00 , C08L1/28
CPC分类号： C08J3/24 , B01D57/02 , C08B15/005 , C08B31/003 , C08B37/00 , G01N27/44747 , C08J2301/00 , C08J2305/00
摘要： A bed of water insoluble, transparent, cross-linked gel, suitable for use in electrophoresis, is formed by dissolving a polysaccharide, including at least one linear polysaccharide such as agarose or hydroxyethyl cellulose, in a suitable solvent, such as water; adding a cross-linking agent, which is not charged nor becomes charged upon contact with water in a pH range of 2 to 11, to the solution; and incubating this mixture in a quiescent state to substantially simultaneously react the polysaccharide and the cross-linking agent and to gel the reaction product into a bed. The polysaccharide is at least one linear polysaccharides, but that linear polysaccharide may also be admixed with other linear polysaccharides and/or at least one non-linear polysaccharide. Synthetic organic polymers may also be admixed in the cross-linking reaction mixture. The polysaccharide, and/or other polymers in the cross-linking reaction mixture may be charged but are preferably uncharged, but if charged, the charge must be low enough so that the charge of the resulting gel, if any, will not interfere with the use of the gel to support an electrophoresis process. When the linear polysaccharide is agarose, the gel has improved elasticity, transparency, and, when the gelled cross-linked product is used to support electrophoresis, improved resolution of small DNA molecules as compared to the results achieved by using an agarose polymer which has not been cross-linked according to this invention.
摘要翻译：通过将包括至少一种线性多糖如琼脂糖或羟乙基纤维素的多糖溶解在合适的溶剂如水中形成适于用于电泳的水不溶性，透明的交联凝胶床，加入交联剂，其在2-11的pH范围内与水接触时不带电并且不会充电; 并将该混合物在静止状态下孵育以基本上同时使多糖和交联剂反应并将反应产物凝胶化成床。多糖是至少一种线性多糖，但也可以将线性多糖与其它线性多糖和/或至少一种非线性多糖混合。合成的有机聚合物也可以在交联反应混合物中混合。可以将交联反应混合物中的多糖和/或其它聚合物加入，但优选不带电荷，但是如果加入，则电荷必须足够低，使得所得凝胶的电荷（如果有的话）将不会干扰使用凝胶来支持电泳过程。当线性多糖是琼脂糖时，凝胶具有改善的弹性，透明度，并且当凝胶交联产物用于支持电泳时，与通过使用不具有琼脂糖聚合物的结果相比，小DNA分子的分辨率提高根据本发明交联。

2. 发明授权

US5319046A Polymers, and their use as gels for electrophoresis 失效
标题翻译：聚合物及其作为电泳凝胶的用途
公开(公告)号：US5319046A
公开(公告)日：1994-06-07
申请号：US328123
申请日：1989-01-31
申请人： Branko Kozulic , Klaus Mosbach
发明人： Branko Kozulic , Klaus Mosbach
IPC分类号： B01D57/02 , C07C233/16 , C08F20/52 , C08F20/58 , C08F20/60 , C08F220/58 , C08L33/04 , C08L33/14 , G01N27/26 , G01N27/447 , C08F20/54 , C08L89/00
CPC分类号： C08F220/58 , G01N27/44747 , Y10S524/916
摘要： A novel synthesis of N-acryloyl-tris-(hydroxymethyl)aminomethane (NAT) is described. The polymerization kinetics and transparency of the poly(NAT) gels crosslinked by a crosslinker (CL) e.g. N,N'-methylenebisacrylamide (Bis), i.e. poly(NAT+Bis) polymer, are shown. Poly(NAT+Bis) gradient (4-24%) gels were prepared and found to resolve native proteins according to their size. The exclusion limit of these gels is over 3.times.10.sup.6 Da. This is more than threefold higher than the exclusion limit of the polyacrylamide gradient gel of the same concentration. Therefore poly(NAT+CL) gels are better than polyacrylamide gels for the resolution of large proteins. It was demonstrated that poly(NAT+CL)-polyacrylamide composite gels could be prepared. The poly(NAT+CL) gels were found to be advantageous also for isoelectric focussing in carrier ampholytes or immobilized pH gradients. Poly(NAT+CL) gels are also very good for separation of nucleic acids. The resolution is particularly good in the range between several hundred to 4-5000 base pairs. Under the optimized conditions, DNA molecules of more than 20 kbases can be perfectly resolved.
摘要翻译： PCT No.PCT / EP88 / 00515 Sec。 371日期1989年1月31日 102（e）日期1989年1月31日PCT Filed 1988年6月10日PCT Pub。出版物WO88 / 09981 日本12月15日，日本。描述了N-丙烯酰基 - 三 - （羟甲基）氨基甲烷（NAT）的新型合成。由交联剂（CL）交联的聚（NAT）凝胶的聚合动力学和透明度，例如 N，N'-亚甲基双丙烯酰胺（Bis），即聚（NAT + Bis）聚合物。制备聚（NAT + Bis）梯度（4-24％）凝胶，发现它们根据其尺寸分解天然蛋白质。这些凝胶的排除极限超过3x106 Da。这比相同浓度的聚丙烯酰胺梯度凝胶的排除极限高三倍以上。因此，聚（NAT + CL）凝胶比用于分解大蛋白质的聚丙烯酰胺凝胶更好。已经证明可以制备聚（NAT + CL） - 聚丙烯酰胺复合凝胶。发现聚（NAT + CL）凝胶对载体两性电解质或固定化pH梯度中的等电聚焦也是有利的。聚（NAT + CL）凝胶也非常适合核酸的分离。该分辨率在数百到4-5000个碱基对之间的范围内特别好。在优化条件下，可以完美解决20多个碱基的DNA分子。

3. 发明授权

US5185466A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5185466A
公开(公告)日：1993-02-09
申请号：US688752
申请日：1991-04-22
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447
CPC分类号： C08F20/58 , G01N27/44747
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：其中R 1是H，CH 2 OH或（CHOH）mCH 2 OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

4. 发明授权

US5458760A Gel composition in gels for submurged gel electrophoresis 失效
标题翻译：凝胶凝胶电泳凝胶组成
公开(公告)号：US5458760A
公开(公告)日：1995-10-17
申请号：US941107
申请日：1992-10-29
申请人： Branko Kozulic
发明人： Branko Kozulic
IPC分类号： G01N27/447 , C25B9/00
CPC分类号： G01N27/44747
摘要： The method of electrophoresis uses for separation of charged species their different mobilities in an electric field. The process is usually carried out by forcing the molecules to migrate through an aqueous gel. The gels are run in essentially two types of electrophoretic units: vertical and horizontal ones. It was found that in the standard submerged electrophoresis units the resolution of DNA in the poly(NAT) gels was never so good as in the vertical system. It was observed that by looking at the gel from above under an angle declined from the vertical, the viewed DNA bands in the gel were much sharper than they were pictured on the photograph taken by a camera positioned more or less vertically above the gel. On basis of this observation it was assumed that the diffuseness of bands seen on the photograph did not originate from a real diffuseness of bands in the gel, but rather from the vertical position of the camera and bending of the bands against the vertical axis. If the bands in the gel could be made essentially vertical, then the resolution taken by the usually positioned camera would be qualitatively better and besides that independent of the sample volume. It is the main goal to prevent such bending of bands in gels during electrophoresis.
摘要翻译： PCT No.PCT / EP92 / 00368 Sec。一九九二年十二月二十四日 102（e）日期1992年12月24日PCT提交1992年2月21日PCT公布。出版物WO92 / 15868 日期：1992年9月17日。电泳方法用于在电场中分离带电物种的不同迁移率。该方法通常通过强迫分子迁移通过水凝胶来进行。凝胶以基本上两种类型的电泳单元运行：垂直和水平单元。发现在标准浸没电泳单元中，聚（NAT）凝胶中DNA的分辨率从未像垂直系统那样好。观察到，通过从垂直方向下降的角度观察来自上方的凝胶，凝胶中观察的DNA条带比通过位于凝胶上方垂直方向定位的照相机拍摄的照片更清晰。在这个观察的基础上，假设在照片上看到的条带的漫射不是源自凝胶中条纹的真实扩散，而是来自照相机的垂直位置和条带相对于垂直轴的弯曲。如果凝胶中的条带可以基本上是垂直的，则通常定位的相机所采取的分辨率将在质量上更好，而且独立于样品体积。在电泳过程中防止胶束弯曲的主要目标是。

5. 发明授权

US5438092A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5438092A
公开(公告)日：1995-08-01
申请号：US145635
申请日：1993-11-04
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447 , C08L39/00
CPC分类号： G01N27/44747 , C08F20/58
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：其中R 1是H，CH 2 OH或（CHOH）mCH 2 OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

6. 发明授权

US5316912A Polyacrylic hydrazides and their applications as glycoprotein reagents 失效
标题翻译：聚丙烯酰肼及其作为糖蛋白试剂的应用
公开(公告)号：US5316912A
公开(公告)日：1994-05-31
申请号：US448105
申请日：1989-12-13
申请人： Urs Heimgartner , Branko Kozulic , Klaus Mosbach
发明人： Urs Heimgartner , Branko Kozulic , Klaus Mosbach
IPC分类号： C08F8/30 , G01N33/532 , G01N33/68 , G01N33/53 , G01N33/543
CPC分类号： G01N33/532 , C08F8/30 , G01N33/6803 , G01N33/6854 , Y10S436/804
摘要： A method for detecting a glycoprotein using a solid support is disclosed where the glycoprotein is oxidized by periodate, polyacrylic polyhydrazide which is a copolymer having repeating units possessing a hydrazide group and repeating units possessing hydroxyl groups is coupled to the oxidized glycoprotein and a glycoenzyme or radioactive compound containing aldehyde groups or activated ketone groups is coupled to the polyacrylic polyhydrazide which allows for detection of the glycoprotein. The glycoprotein may be directly attached to the solid support or may be bound to an antigen which is immobilized on the solid support.
摘要翻译：公开了一种使用固体支持物检测糖蛋白的方法，其中糖蛋白被高碘酸酯聚丙烯酰胺酰胺氧化，其是具有重复单元的具有酰肼基团的共聚物，并且具有羟基的重复单元与氧化糖蛋白和糖酵解或放射性含有醛基或活化酮基的化合物与能够检测糖蛋白的聚丙烯酸聚酰肼偶联。糖蛋白可以直接连接到固体支持物上，或者可以结合固定在固体支持物上的抗原。

7. 发明授权

US5278270A Hydrophilic and amphiphatic monomers, their polymers and gels and hydrophobic electrophoresis 失效
标题翻译：亲水和双亲单体，其聚合物和凝胶和疏水电泳
公开(公告)号：US5278270A
公开(公告)日：1994-01-11
申请号：US972343
申请日：1992-11-06
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： C08F20/58 , G01N27/447 , C07C233/20
CPC分类号： G01N27/44747 , C08F20/58
摘要： An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;R.sub.2 is monohydroxyalkyl, polyhydroxyalkyl or hydrocarbon radical;R.sub.3 is H or CH.sub.3 ; andn is an integer of 1-4The acrylic monomer can be polymerized by a free radical polymerization, either alone or with other compounds and materials having polymerizable double bonds. The so formed polymers are linear or branched (cross-linked). The cross-linked polymers are usually in the form of gels. The gels can be polymerized in different forms and shapes, i.e., beads, thin sheets, rods, blocks, etc. and are useful as separation media, e.g., the transparent gels are useful as an anticonvective matrix in electrophoresis, whereas the opaque gels prepared in a bead form are useful in chromatography.
摘要翻译：丙烯酸类单体，其是其伯或仲氨基已被丙烯酰基或甲基丙烯酰基官能团衍生的氨基糖醇。氨基可以连接到五或六碳糖醇的任何碳上。丙烯酸类单体具有下式：（*化学结构*）其中R1是H，CH2OH或（CHOH）mCH2OH，m是1或2; R2是单羟基烷基，多羟基烷基或烃基; R3是H或CH3; 并且n是1-4的整数。丙烯酸类单体可以通过自由基聚合单独或与其它化合物和具有可聚合双键的材料聚合。如此形成的聚合物是直链或支链（交联的）。交联聚合物通常是凝胶的形式。凝胶可以以不同的形式和形状聚合，即珠，薄片，棒，块等，并且可用作分离介质，例如，透明凝胶可用作电泳中的抗ive基质，而制备的不透明凝胶珠粒形式可用于色谱法。

8. 发明授权

US07025864B2 Method and apparatus for recovering target molecules from a gel containing said target molecules 失效
标题翻译：从含有所述靶分子的凝胶中回收靶分子的方法和装置
公开(公告)号：US07025864B2
公开(公告)日：2006-04-11
申请号：US10383683
申请日：2003-03-10
申请人： Branko Kozulic
发明人： Branko Kozulic
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/4473
摘要： A preparative electrophoresis apparatus suitable for recovery of molecules from gels comprises two spaced apart electrode compartments connected by a conduit that descends from the bottom of the electrode compartments in a V-shaped or U-shaped form. The conduit serves as a reservoir for collecting electroeluted molecules. Two electrophoresis buffers are used, a first one of a low concentration and density, and a second one of a high concentration and density. The electrode compartments are filled with the first electrophoresis buffer solution while the conduit is filled with the second electrophoresis buffer solution. Under the influence of an electric field, the molecules exit the gel and concentrate in the high concentration buffer. After a certain time, usually a time that is sufficient for driving substantially all desired molecules out of the gel, the electric field is switched off. The high concentration buffer containing the electroeluted molecules is withdrawn from the conduit. The target molecules may be recovered from the high concentration buffer solution.
摘要翻译：适用于从凝胶回收分子的制备型电泳装置包括两个间隔开的电极隔室，其通过导管以V形或U形形式从电极隔室的底部下降。导管用作收集电泳分子的储存器。使用两个电泳缓冲液，低浓度和密度的第一个，高浓度和密度的第二个。在用第二电泳缓冲溶液填充导管的同时，用第一电泳缓冲溶液填充电极室。在电场的影响下，分子离开凝胶并浓缩在高浓度缓冲液中。在一定时间之后，通常是将基本上所有需要的分子从凝胶中驱出的时间足够，电场被切断。含有电洗脱分子的高浓度缓冲液从导管中取出。靶分子可以从高浓度缓冲溶液中回收。

9. 发明申请

US20050250220A1 Isoelectric particles and uses thereof 失效
标题翻译：等电粒子及其用途
公开(公告)号：US20050250220A1
公开(公告)日：2005-11-10
申请号：US10840347
申请日：2004-05-07
申请人： Branko Kozulic
发明人： Branko Kozulic
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/551 , G01N33/561
CPC分类号： G01N33/54346 , C12Q1/6816 , G01N27/44795 , G01N33/561 , C12Q2565/125 , C12Q2563/149 , C12Q2527/15
摘要： A plurality of particles of from about 5 nm to 100 μm possessing predetermined isoelectric points in the pH range from about 2.5 to 11 is used in a method of detection of a plurality of analytes, wherein the isoelectric particles of each isoelectric point further contain a label and a member of a binding pair capable of interacting with a selected analyte. The particles that formed specific binding pairs are recovered and separated by isoelectric focusing, followed by the detection of the labels associated with the particles. A flow cytometer may be used as a detector of the isoelectric particles.
摘要翻译：在约5〜100μm的多个颗粒中，在约2.5-11的pH范围内具有预定的等电点，用于检测多种分析物的方法，其中每个等电点的等电粒子还含有标记以及能够与所选分析物相互作用的结合对的成员。形成特异性结合对的颗粒被回收并通过等电聚焦分离，随后检测与颗粒相关的标记。流式细胞仪可用作等电粒子的检测器。

10. 发明授权

US5259943A Apparatus and method for submerged gel electrophoresis 失效
标题翻译：浸没式凝胶电泳装置及方法
公开(公告)号：US5259943A
公开(公告)日：1993-11-09
申请号：US909831
申请日：1992-07-07
申请人： Branko Kozulic , Urs Heimgartner
发明人： Branko Kozulic , Urs Heimgartner
IPC分类号： G01N27/447 , B01D61/00
CPC分类号： G01N27/44704
摘要： Electrophoresis apparatus for conducting electrophoresis in submerged gels, comprises a plurality of compartments including at least one gel compartment and at least one reservoir compartment; a plate as the bottom of a gel compartment, electrodes only in a gel compartment; the electrodes arranged in such a way that during operation the created electric field is confined essentially within a rectangular box, the said rectangular box defined on sides by side walls or barriers, on top by air and on bottom by the plate; means to circulate buffer; and barriers in a gel compartment mounted on the plate in front of buffer circulation openings.
摘要翻译：用于在浸没凝胶中进行电泳的电泳装置包括多个隔室，其包括至少一个凝胶室和至少一个贮存室; 作为凝胶室底部的板，电极仅在凝胶室中; 所述电极以这样的方式布置，使得在操作期间，所产生的电场基本上被限制在矩形盒内，所述矩形盒由侧壁或屏障侧面限定，顶部由空气限定，底部由板限定; 循环缓冲的手段以及在缓冲循环开口前面安装在板上的凝胶室中的阻挡层。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式