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1. 发明授权

US06455259B2 Methods for detecting, selecting and cloning agents that degrade or promote degradation of DNA 失效
标题翻译：用于检测，选择和克隆降解或促进DNA降解的试剂的方法
公开(公告)号：US06455259B2
公开(公告)日：2002-09-24
申请号：US09903986
申请日：2001-07-13
申请人： Thomas L. Mattson
发明人： Thomas L. Mattson
IPC分类号： C12Q168
CPC分类号： C12Q1/68 , C12N15/1034
摘要： A method is described for detecting, selecting, and cloning agents that degrade DNA or promote DNA degradation. The method utilizes extra-chromosomal replicons whose replication is dependent on degradation of a host cell's DNA to screen for agents leading to degradation of cellular DNA. An agent which promotes degradation of a host cell's DNA enables the replicons to replicate, which signals the presence of agents that promote the cellular DNA degradation and allows for the isolation and amplification of such agents.
摘要翻译：描述了用于检测，选择和克隆降解DNA或促进DNA降解的试剂的方法。该方法利用染色体外复制子，其复制依赖于宿主细胞DNA的降解以筛选导致细胞DNA降解的试剂。促进宿主细胞DNA降解的试剂能使复制子复制，这表明促进细胞DNA降解的试剂的存在，并允许这些试剂的分离和扩增。

2. 发明授权

US5559018A Inviable phages, their production and DNA thereof 失效
标题翻译：不可侵犯的噬菌体，其生产和DNA
公开(公告)号：US5559018A
公开(公告)日：1996-09-24
申请号：US946033
申请日：1992-09-15
申请人： Thomas L. Mattson , Richard Epstein
发明人： Thomas L. Mattson , Richard Epstein
IPC分类号： C12N15/70 , C12N15/00 , C12N15/63
CPC分类号： C12N15/70
摘要： Inviable T4 phage-like particles capable of directing the expression of large non-T4 DNA fragments from T4 expression control sequences are produced. Thus, E. coli harboring pBR322 derivatives containing cloned T4 gene 23 DNA sequences were infected with T4 phage carrying a deletion of the denB gene. Homology-dependent recombination results in the production of inviable phage-like particles containing DNA molecules composed of multiple, tandemly repeated copies of entire plasmid molecules covalently linked to single copies of normal phage genes. The yield of these inviable particles, intially low, was increased by means of a reiterated infection process that involves the use of a cloned T4 origin of replication. When T4 gene 32 expression control sequences linked in proper orientation to a DNA sequence coding for the non-T4 protein .beta.-galactosidase were also cloned in one such pBR322 derivative (pVH773), inviable phage particles capable of directing the synthesis of enzymatically active .beta.-galactosidase were produced. The present process is applicable to other T-even bacterio-phages.
摘要翻译：产生能够引导来自T4表达调控序列的大的非T4 DNA片段表达的不可维生的T4噬菌体样颗粒。因此，含有克隆的T4基因23个DNA序列的pBR322衍生物的大肠杆菌用携带denB基因缺失的T4噬菌体感染。同源性依赖性重组导致产生含有DNA分子的易发生的噬菌体样颗粒，其由与正常噬菌体基因的单拷贝共价连接的整个质粒分子的多个串联重复拷贝组成。通过涉及使用克隆的T4复制起点的重复的感染过程，这些不可侵犯的颗粒的产量是非常低的。当将T4基因32表达控制序列与编码非T4蛋白质β-半乳糖苷酶的DNA序列连接的表达调控序列也克隆在一个这样的pBR322衍生物（pVH773）中时，能够引导酶活性β- 产生半乳糖苷酶。本方法适用于其他T-even细菌噬菌体。

3. 发明授权

US06500611B2 Inviable virus particles as scaffolds for constructing flexible detection systems 失效
标题翻译：不可见的病毒颗粒作为构建灵活检测系统的支架
公开(公告)号：US06500611B2
公开(公告)日：2002-12-31
申请号：US09931094
申请日：2001-08-17
申请人： Thomas L. Mattson
发明人： Thomas L. Mattson
IPC分类号： C12Q170
CPC分类号： C12N7/00 , C07K14/4721 , C07K2319/00 , C12N9/1007 , C12N2795/10122 , C12N2795/10162 , G01N33/58
摘要： An innovative detection system for detecting small numbers of target analytes is disclosed. This system provides a novel method for attaching multiple copies of reporter groups to a single site on an analyte of interest. This system preferably comprises a virus capsid enclosing multiple detectable reporter groups, and a linking molecule which is capable of linking the capsid to the analyte of interest.
摘要翻译：公开了一种用于检测少量目标分析物的创新检测系统。该系统提供了一种将多个报告基团拷贝附着到感兴趣的分析物上的单个位点的新方法。该系统优选包含包围多个可检测报道基团的病毒衣壳，以及能够将衣壳与感兴趣的分析物连接的连接分子。

4. 发明授权

US06268139B1 Methods for detecting, selecting and cloning agents that degrade or promote degradation of DNA 失效
标题翻译：用于检测，选择和克隆降解或促进DNA降解的试剂的方法
公开(公告)号：US06268139B1
公开(公告)日：2001-07-31
申请号：US09261764
申请日：1999-03-03
申请人： Thomas L. Mattson
发明人： Thomas L. Mattson
IPC分类号： C12Q168
CPC分类号： C12Q1/68 , C12N15/1034
摘要： A method is described for detecting, selecting, and cloning agents that degrade DNA or promote DNA degradation. The method utilizes extra-chromosomal replicons whose replication is dependent on degradation of a host cell's DNA to screen for agents leading to degradation of cellular DNA. An agent which promotes degradation of a host cell's DNA enables the replicons to replicate, which signals the presence of agents that promote the cellular DNA degradation and allows for the isolation and amplification of such agents.
摘要翻译：描述了用于检测，选择和克隆降解DNA或促进DNA降解的试剂的方法。该方法利用染色体外复制子，其复制依赖于宿主细胞DNA的降解以筛选导致细胞DNA降解的试剂。促进宿主细胞DNA降解的试剂能使复制子复制，这表明促进细胞DNA降解的试剂的存在，并允许这些试剂的分离和扩增。

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