会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 8. 发明授权
    • Enzymatic conversion of .alpha.-hydroxynitriles to the corresponding .alpha
.
    • α-羟基腈向相应的α-羟基酰胺,酸或酸式盐的酶转化
    • US5866379A
    • 1999-02-02
    • US790675
    • 1997-01-28
    • Richard P. BurlingameJames R. MillisFernando Sanchez-RieraThomas F. BlackburnAlan D. Grund
    • Richard P. BurlingameJames R. MillisFernando Sanchez-RieraThomas F. BlackburnAlan D. Grund
    • C12P7/42C12P11/00C12P13/02
    • C12R1/01C12P11/00C12P13/02C12P7/42Y10S435/822
    • Enzymes derived from the isolated and substantially purified microorganisms of the present invention, designated herein as strains 52 and 56wt, are capable of hydrating nitriles such as 2-hydroxy-4-(methylthio)-butanenitrile (HMB-nitrile) to their corresponding amides, and further, of hydrolyzing amides such as 2-hydroxy-4-(methylthio)-butaneamide (HMB-amide) to their corresponding carboxylic acids. Advantageously, the nitrile hydratase of these strains is not substantially inhibited by the .alpha.-hydroxybutyramide product being formed; rather, this enzyme maintains the ability to hydrate an .alpha.-hydroxybutyronitrile to its corresponding amide even at high amide concentrations, including at saturating amide conditions. As such, enzymes derived from strains 52 and 56wt are particularly suited for commercial use in preparing agrichemical intermediates such as HMB-amide. HMB-amide may, in turn, be hydrolyzed through traditional or enzymatic conversion methods to form HMB-acid or salts thereof, which are useful as methionine substitutes in feeds for domestic animals.
    • 衍生自本发明的分离和基本纯化的微生物的酶,其在本文中称为菌株52和56wt,能够将腈如2-羟基-4-(甲硫基)丁腈(HMB-腈)水合至其相应的酰胺, 此外,将酰胺如2-羟基-4-(甲硫基) - 丁酰胺(HMB-酰胺)水解成其相应的羧酸。 有利地,这些菌株的腈水合酶基本上不被形成的α-羟基丁酰胺产物抑制; 相反,该酶保持即使在高酰胺浓度(包括在饱和酰胺条件下)将α-羟基丁腈水解成其相应的酰胺的能力。 因此,源自菌株52和56wt的酶特别适用于制备农药中间体如HMB-酰胺的商业用途。 HMB-酰胺又可以通过传统的或酶促转化方法水解形成HMB-酸或其盐,它们可用作家畜饲料中的甲硫氨酸替代物。