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1. 发明授权

US08663990B2 Measurement method utilizing internal standard substance 有权
标题翻译：使用内标物质的测量方法
公开(公告)号：US08663990B2
公开(公告)日：2014-03-04
申请号：US13318079
申请日：2010-04-27
申请人： Naoyuki Yamamoto , Tatsuo Kurosawa
发明人： Naoyuki Yamamoto , Tatsuo Kurosawa
IPC分类号： G01N27/26 , C07K1/14 , C07K1/26 , C07D403/06
CPC分类号： G01N27/44704
摘要： A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups.
摘要翻译：本发明的主题是提供一种在分析物是蛋白质或化合物的电泳中使用内标物质的测定方法。本发明涉及通过电泳进行分析物的测定方法，其特征在于，通过使用分子内具有3个以上阴离子基团的化合物I的组合作为内标物质（1）来鉴定分析物的峰和其中1〜3个所述化合物I的阴离子基团被阳离子基团取代的化合物II，或（2）分子中具有3个以上阳离子基团的化合物III和化合物IV的组合，其中1〜所述化合物III的3个阳离子基团已被阳离子基团取代。

2. 发明申请

US20120043207A1 MEASUREMENT METHOD UTILIZING INTERNAL STANDARD SUBSTANCE 有权
标题翻译：使用内部标准物质的测量方法
公开(公告)号：US20120043207A1
公开(公告)日：2012-02-23
申请号：US13318079
申请日：2010-04-27
申请人： Naoyuki Yamamoto , Tatsuo Kurosawa
发明人： Naoyuki Yamamoto , Tatsuo Kurosawa
IPC分类号： G01N27/447
CPC分类号： G01N27/44704
摘要： A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups.
摘要翻译：本发明的主题是提供一种在分析物是蛋白质或化合物的电泳中使用内标物质的测定方法。本发明涉及通过电泳进行分析物的测定方法，其特征在于，通过使用分子内具有3个以上阴离子基团的化合物I的组合作为内标物质（1）来鉴定分析物的峰和其中1〜3个所述化合物I的阴离子基团被阳离子基团取代的化合物II，或（2）分子中具有3个以上阳离子基团的化合物III和化合物IV的组合，其中1〜所述化合物III的3个阳离子基团已被阳离子基团取代。

3. 发明申请

US20090226346A1 DISPENSING DEVICE 审中-公开
标题翻译：分配设备
公开(公告)号：US20090226346A1
公开(公告)日：2009-09-10
申请号：US12442436
申请日：2007-09-21
申请人： Takahiro Miyato , Tatsuo Kurosawa , Tomohisa Kawabata , Masayoshi Hayashi
发明人： Takahiro Miyato , Tatsuo Kurosawa , Tomohisa Kawabata , Masayoshi Hayashi
IPC分类号： B01L3/02
CPC分类号： G01N35/1016 , G01N21/11 , G01N35/028 , G01N2035/1034
摘要： A dispensing device discharges a liquid into a well of a microchip, which includes the well having an opening at the upper end, and a microchannel in fluid communication with the lower end of the well. The well has a bottom portion formed by an annular step portion projecting inward at the lower end. The dispensing device includes: a nozzle having a tip opening for suctioning and discharging a liquid therethrough; a pump for supplying suction pressure and discharge pressure to the nozzle; moving unit for causing relative movement of the nozzle at least in depth direction of the well; and control unit for causing the moving unit to cause the relative movement of the nozzle until the tip opening reaches the bottom portion, and then causing the liquid to be discharged such that the liquid first contacts a bottom surface and/or an inner circumferential surface of the bottom portion.
摘要翻译：分配装置将液体排放到微芯片的井中，该微芯片包括在上端具有开口的井以及与井的下端流体连通的微通道。井具有由在下端向内突出的环状台阶部形成的底部。分配装置包括：喷嘴，其具有用于吸入和排出液体的尖端开口; 用于向喷嘴提供吸入压力和排出压力的泵; 移动单元，用于至少在井的深度方向上引起喷嘴的相对运动; 以及控制单元，用于使所述移动单元引起所述喷嘴的相对运动，直到所述尖端开口到达所述底部部分，然后使所述液体排出，使得所述液体首先接触到所述液体的底表面和/或内周面底部。

4. 发明申请

US20090223012A1 LIQUID SUCTION DEVICE 审中-公开
标题翻译：液体吸收装置
公开(公告)号：US20090223012A1
公开(公告)日：2009-09-10
申请号：US12442480
申请日：2007-09-21
申请人： Daisuke Hibe , Tatsuo Kurosawa , Tomohisa Kawabata , Masayoshi Hayashi
发明人： Daisuke Hibe , Tatsuo Kurosawa , Tomohisa Kawabata , Masayoshi Hayashi
IPC分类号： A47L9/02
CPC分类号： G01N35/1016 , G01N35/028 , G01N2035/00158 , G01N2035/1034
摘要： A liquid suction device used to suction a liquid contained in a well of a microchip which includes the well and a microchannel in fluid communication with the well. The liquid suction device includes: a suction nozzle having a tip opening at a tip thereof and being connected to piping at a rear end thereof, the suction nozzle suctioning the liquid through the tip opening; a pump connected to the piping, the pump supplying a suction pressure to the suction nozzle; and a driving unit for moving the suction nozzle relative to the well. In liquid suction device, the suction nozzle is moved by the driving unit until the tip opening contacts a bottom surface of the well while the suction nozzle applies a suction force produced by the suction pressure, thereby enabling suction of the liquid from the well and/or the microchannel.
摘要翻译：用于抽吸包含在包括井的微芯片的井中的液体的液体抽吸装置和与井流体连通的微通道。吸液装置包括：吸嘴，其顶端具有尖端开口，并在其后端与管道连接，吸嘴通过尖端开口吸液; 连接到所述管道的泵，所述泵向所述吸嘴提供吸入压力; 以及用于相对于井移动吸嘴的驱动单元。在液体抽吸装置中，吸嘴由驱动单元移动，直到尖端开口接触到井的底表面，而吸嘴施加由吸入压力产生的吸力，从而能够从井和/ 或微通道。

5. 发明授权

US08580097B2 Isotachophoresis of blood-derived samples 有权
标题翻译：血液样品的等速电泳
公开(公告)号：US08580097B2
公开(公告)日：2013-11-12
申请号：US12709423
申请日：2010-02-19
申请人： Tatsuo Kurosawa , Mitsuo Watanabe , Takuma Ohtsubo
发明人： Tatsuo Kurosawa , Mitsuo Watanabe , Takuma Ohtsubo
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44773 , G01N27/44726
摘要： Methods are provided for forming a complex comprising an analyte (or an analyte analogue) from a blood-derived sample and labeling substances, and separating the complex from excess labeling substances and coexisting substances from the blood-derived sample, in a rapid, simple, convenient, and highly precise isotachophoresis (ITP) process by adding 2-(N-morpholino)ethane sulfonate (MES) salt and/or glutamate salt to the ITP sample. Methods are also provided for measuring the analyte in blood-derived samples with high precision and high sensitivity, based on the amount of the complex separated or the amount of uncomplexed labeling substance-containing molecules.
摘要翻译：提供了用于形成包含来自血液来源样品的分析物（或分析物类似物）和标记物质的复合物的复合物的方法，并且以快速，简单的方式将复合物与来自血液衍生的样品的过量标记物质和共存物质分离，通过向ITP样品中加入2-（N-吗啉代）乙烷磺酸盐（MES）盐和/或谷氨酸盐，方便和高度精确的等速电泳（ITP）方法。还提供了用于以高精度和高灵敏度测量血液来源的样品中的分析物的方法，基于分离的复合物的量或未复合的含标记物质的分子的量。

6. 发明授权

US08518350B2 Reagent container 有权
标题翻译：试剂容器
公开(公告)号：US08518350B2
公开(公告)日：2013-08-27
申请号：US12680093
申请日：2008-09-24
申请人： Tatsuyuki Denawa , Masayoshi Hayashi , Tatsuo Kurosawa , Masahiro Satomura , Mitsuo Watanabe
发明人： Tatsuyuki Denawa , Masayoshi Hayashi , Tatsuo Kurosawa , Masahiro Satomura , Mitsuo Watanabe
IPC分类号： B01L3/00
CPC分类号： G01N35/1002 , B01L3/523 , B01L3/527 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0672 , B01L2300/0861 , G01N35/026 , G01N35/1079 , G01N2035/0436
摘要： A reagent container having a plurality of open holding sections, each holding a reagent and into which a probe is inserted to suck the reagent. An opening of each of the holding sections is sealed with at least one sheet-like seal member. The reagent container includes a container body and a lid having a plurality of hollow piercing sections, formed on a lower surface of the lid, protruding downward for piercing the seal member at each of the openings, and openings, formed in an upper surface of the lid, communicating with piercing sections respectively to allow the probe to be inserted therethrough or withdrawn therefrom.
摘要翻译：一种试剂容器，其具有多个开放保持部，每个保持试剂，插入探针以吸引试剂。每个保持部分的开口用至少一个片状密封件密封。试剂容器包括容器主体和具有多个中空穿刺部的盖，该盖形成在盖的下表面上，向下突出以刺穿每个开口处的密封构件，并且形成在开口的上表面盖分别与刺穿部分连通以允许探针插入其中或从其中抽出。

7. 发明申请

US20120046474A1 PYRAZOLE-BASED CYANINE DYE CONTAINING QUATERNARY AMMONIUM CATION 有权
公开(公告)号：US20120046474A1
公开(公告)日：2012-02-23
申请号：US13318089
申请日：2010-04-27
申请人： Naoyuki Yamamoto , Tatsuo Kurosawa , Satoshi Hasaba , Matsuhiro Date
发明人： Naoyuki Yamamoto , Tatsuo Kurosawa , Satoshi Hasaba , Matsuhiro Date
IPC分类号： C07D403/06
CPC分类号： C07D403/06 , C09B23/083
摘要： ProblemProvided is a novel cyanine dye derivative having high fluorescence intensity in a short wavelength region, a structure where a pyrazole skeleton and an indole skeleton are bound to a polymethine chain, and also a controllable migration velocity by further introducing a quaternary ammonium cation into a molecule having said structure.SolutionThe present invention is the invention of a compound represented by the general formula [1] or a salt thereof: [wherein R1 to R6 each independently represent an alkyl group having, as a substituent, a group represented by the general formula [101]: (wherein R101 to R104 each independently represent a hydrogen atom or an alkyl group of C1 to C3 and m represents an integer from 2 to 6, and two or three of R102 to R104 together with a nitrogen atom to which they are bound may form a heterocyclic ammonium cation); or a group derived from a carboxylic acid represented by the general formula [2] which may have an amide bond: —COOR12 [2] (wherein R12 represents a hydrogen atom, an alkyl group of C1 to C10, an alkali metal atom, an organic ammonium ion, an ammonium ion or anion); or an alkyl group which may have, as a substituent, a group derived from a sulfonic acid represented by the general formula [3] which may have an amide bond: —SO3R13 [3] (wherein R13 represents a hydrogen atom, an alkali metal atom, an organic ammonium ion, an ammonium ion or anion); R7 to R10 each independently represent an alkyl group, an alkynyl group, an aryl group, an alkoxy group, an aryloxy group, an alkylsulfonyl group, an arylsulfonyl group, an amino group, a group derived from a carboxylic acid represented by the general formula [2], a group derived from a sulfonic acid represented by the general formula [3], a halogen atom, a hydrogen atom, a hydroxyl group, a cyano group or a nitro group; R11 represents a hydrogen atom, an alkyl group, an alkynyl group or an aryl group, and n represents an integer from 0 to 3, and at least one of R1 to R6 is an alkyl group having, as a substituent, a group represented by the general formula [101]].

8. 发明申请

US20100233035A1 REAGENT CONTAINER 有权
标题翻译：试剂容器
公开(公告)号：US20100233035A1
公开(公告)日：2010-09-16
申请号：US12680093
申请日：2008-09-24
申请人： Tatsuyuki Denawa , Masayoshi Hayashi , Tatsuo Kurosawa , Masahiro Satomura , Mitsuo Watanabe
发明人： Tatsuyuki Denawa , Masayoshi Hayashi , Tatsuo Kurosawa , Masahiro Satomura , Mitsuo Watanabe
IPC分类号： B01L3/00
CPC分类号： G01N35/1002 , B01L3/523 , B01L3/527 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0672 , B01L2300/0861 , G01N35/026 , G01N35/1079 , G01N2035/0436
摘要： A reagent container having a plurality of open holding sections, each holding a reagent and into which a probe is inserted to suck the reagent. An opening of each of the holding sections is sealed with at least one sheet-like seal member. The reagent container includes a container body and a lid having a plurality of hollow piercing sections, formed on a lower surface of the lid, protruding downward for piercing the seal member at each of the openings, and openings, formed in an upper surface of the lid, communicating with piercing sections respectively to allow the probe to be inserted therethrough or withdrawn therefrom.
摘要翻译：一种试剂容器，其具有多个开放保持部，每个保持试剂，插入探针以吸引试剂。每个保持部分的开口用至少一个片状密封件密封。试剂容器包括容器主体和具有多个中空穿刺部的盖，该盖形成在盖的下表面上，向下突出以刺穿每个开口处的密封构件，并且形成在开口的上表面盖分别与刺穿部分连通以允许探针插入其中或从其中抽出。

9. 发明授权

US08546586B2 Pyrazole-based cyanine dye containing quaternary ammonium cation 有权
标题翻译：含有季铵阳离子的吡唑类花青染料
公开(公告)号：US08546586B2
公开(公告)日：2013-10-01
申请号：US13318089
申请日：2010-04-27
申请人： Naoyuki Yamamoto , Tatsuo Kurosawa , Satoshi Hasaba , Matsuhiro Date
发明人： Naoyuki Yamamoto , Tatsuo Kurosawa , Satoshi Hasaba , Matsuhiro Date
IPC分类号： C07D403/06
CPC分类号： C07D403/06 , C09B23/083
摘要： A cyanine dye derivative having high fluorescence intensity in a short wavelength region, and also a controllable migration velocity by further introducing a quaternary ammonium cation. A compound having general formula [1] or a salt thereof: wherein R1 to R6 each independently represent an alkyl group having, as a substituent, a group represented by the general formula [101]:
摘要翻译：在短波长区域具有高荧光强度的花青染料衍生物，并且通过进一步引入季铵阳离子也可控制迁移速度。具有通式[1]的化合物或其盐：其中R 1至R 6各自独立地表示具有由通式[101]表示的基团作为取代基的烷基：

10. 发明申请

US20100270157A1 ISOTACHOPHORESIS 有权
公开(公告)号：US20100270157A1
公开(公告)日：2010-10-28
申请号：US12709423
申请日：2010-02-19
申请人： Tatsuo Kurosawa , Mitsuo Watanabe , Takuma Ohtsubo
发明人： Tatsuo Kurosawa , Mitsuo Watanabe , Takuma Ohtsubo
IPC分类号： G01N27/447
CPC分类号： G01N27/44773 , G01N27/44726
摘要： Problems to be SolvedThere is provided a method for separating the complex containing the analyte (or the analogue) in the blood-derived sample and the labeling substances, etc., and coexisting substances in a blood-derived sample, rapidly, simply and conveniently and in high precision by a isotachophoresis (ITP); and a measuring method for the analyte in said sample in high precision and in high sensitivity, based on amount of the complex separated or amount of the free labeling substance-containing molecules, which were not involved in formation of said complex.Method for Solving the ProblemA method for separating a complex which comprises, concentrating the following complex, while separating a complex containing an analyte (or the analogue) in the blood-derived sample, the substances which are capable of forming the complex (CFS) with said analyte and the substances which are capable of changing electrophoretic mobility of the analyte (or the analogue), from the free labeling substance-containing molecules (the labeled CFS, the labeled analogue and the analyte-labeled CFS complex) which were not involved in formation of the complex including the labeling substances and the coexisting substances in said sample, by ITP, in the presence of an 2-(N-morpholino)ethane sulfonate (MES) ion and/or a glutamate ion.
摘要翻译：要解决的问题提供一种方法，用于将血源性样品中的分析物（或类似物）和标记物质等的复合物以及血液样品中的共存物质快速，简便地并通过等速电泳（ITP）以高精度进行; 以及基于未参与形成所述复合物的含有分子量或含游离标记物质的分子的量，以高精度和高灵敏度的方式分析所述样品中的分析物。解决问题的方法一种分离复合物的方法，其包括在分离含有来自血液的样品中的分析物（或类似物）的复合物的同时浓缩以下复合物，能够形成复合物（CFS）的物质，所述分析物和能够从未涉及的游离标记物质的分子（标记的CFS，标记的类似物和分析物标记的CFS复合物）改变分析物（或类似物）的电泳迁移率的物质通过ITP在2-（N-吗啉代）乙烷磺酸盐（MES）离子和/或谷氨酸根离子的存在下形成包含所述样品中的标记物质和共存物质的复合物。

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