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1. 发明申请

US20090223823A1 METHOD OF SEPARATING PROTEIN, METHOD OF STAINING PROTEIN AND LIQUID PROTEIN-STAINING AGENT AND PROTEIN-STAINING KIT TO BE USED IN THESE METHODS 审中-公开
标题翻译：分离蛋白质的方法，蛋白质和液体蛋白质染色剂的染色方法以及用于这些方法的蛋白质染色试剂盒
公开(公告)号：US20090223823A1
公开(公告)日：2009-09-10
申请号：US11720927
申请日：2005-12-06
申请人： Ichiji Namatame , Yoshinori Ishii , Yoshimasa Saito , Takayoshi Komatsu , Yasuhiro Ogawa , Takashi Shibata , Hideki Kinoshita , Kenji Yokoyama
发明人： Ichiji Namatame , Yoshinori Ishii , Yoshimasa Saito , Takayoshi Komatsu , Yasuhiro Ogawa , Takashi Shibata , Hideki Kinoshita , Kenji Yokoyama
IPC分类号： B01D57/02 , C07K2/00 , G01N33/00
CPC分类号： G01N27/44726
摘要： A method of separating a protein by gel electrophoresis, which comprises a staining step for bringing a protein-containing sample into contact with a staining liquid containing a staining agent, a surfactant and a buffer solution, and an electrophoresis step for subjecting the protein-containing sample after the staining step to gel electrophoresis.According to the present invention, using a staining liquid containing a surfactant such as SDS and the like, a protein-containing sample can be stained in a short time, and can develop color with high sensitivity. In addition, since an excess staining agent migrates earlier than protein by electrophoresis, a washing operation is not necessary. Furthermore, by staining, after the first dimension electrophoresis, with a staining liquid containing a surfactant such as SDS and the like, the second dimension electrophoresis can be performed immediately. As a result, the number of steps can be reduced as compared to conventional protein separation methods, and the separation operation can be simplified. Hence, a method capable of staining and separating a protein conveniently and quickly by electrophoresis can be provided.
摘要翻译：一种通过凝胶电泳分离蛋白质的方法，其包括使含蛋白质的样品与含有染色剂，表面活性剂和缓冲溶液的染色液体接触的染色步骤，以及将含蛋白质的样品染色步骤后进行凝胶电泳。根据本发明，使用含有SDS等表面活性剂的染色液，可以在短时间内对含蛋白质的样品进行染色，可以以高灵敏度显色。此外，由于过量的染色剂通过电泳比蛋白质迁移更早，所以不需要洗涤操作。此外，通过染色，在第一维电泳之后，用含有诸如SDS等的表面活性剂的染色液进行染色，可以立即进行第二维电泳。结果，与常规蛋白质分离方法相比，可以减少步骤数，并且可以简化分离操作。因此，可以提供通过电泳方便快速地染色和分离蛋白质的方法。

2. 发明授权

US4268614A Method of making printed circuit board 失效
标题翻译：制作印刷电路板的方法
公开(公告)号：US4268614A
公开(公告)日：1981-05-19
申请号：US967388
申请日：1978-12-07
申请人： Tamotsu Ueyama , Yukihiko Wada , Masaji Homma , Hitoshi Aisawa , Takayoshi Komatsu , Tatsuhisa Shibata , Hiroharu Kamiyama
发明人： Tamotsu Ueyama , Yukihiko Wada , Masaji Homma , Hitoshi Aisawa , Takayoshi Komatsu , Tatsuhisa Shibata , Hiroharu Kamiyama
IPC分类号： H05K3/18 , H05K3/42 , G03C5/00
CPC分类号： H05K3/184 , H05K3/42 , H05K3/428 , H05K2203/025 , H05K2203/0565 , H05K2203/0574 , H05K2203/1383
摘要： A novel circuit board in which circuit portions including pad portions, through-hole portions and conductors are formed, at least at the pad portions and the through-hole portions of the circuit, in the vacant portions defined by a cured photopolymeric resin composition and the vacant portions are plated with electroless copper. When only the pad portions and through-hole portions are plated with electroless copper, the conductors are all covered with the resin composition, thereby enabling fears of mechanical and chemical damages to be eliminated. When all the circuit portions are plated with electroless copper, the amount of copper required can be considerably reduced.Such circuit board is prepared by applying onto a laminate with or without copper clad thereon a specific photopolymerizable resin composition, irradiating the resin composition-applied laminate with ultraviolet rays through a pattern mask, treating the thus irradiated laminate with a solvent to remove the unexposed portions of resin composition and applying an electroless copper plating to the unexposed portions. A durable and reliable printed circuit board having a high pecision and high density circuit pattern can be provided very simply, easily at low cost.
摘要翻译：一种新颖的电路板，其中，在由固化的光聚合树脂组合物限定的空位部分中至少在电路的焊盘部分和通孔部分处形成包括焊盘部分，通孔部分和导体的电路部分，空位部分镀有化学镀铜。当只有焊盘部分和通孔部分镀有无电镀铜时，导体都被树脂组合物覆盖，从而可以消除机械和化学损坏的恐惧。当所有电路部分都镀有化学镀铜时，所需的铜的量可以大大降低。通过在具有或不具有铜的层压板上涂布特定的光聚合性树脂组合物，通过图案掩模对紫外线照射树脂组合物施加的层压体，用溶剂处理这样照射的层压体以除去未曝光部分来制备这种电路板的树脂组合物，并对未曝光部分施加化学镀铜。可以非常简单，容易地以低成本提供具有高精度和高密度电路图案的耐用且可靠的印刷电路板。

3. 发明申请

US20050016242A1 Continous hot-rolling facility 审中-公开
标题翻译：连续热轧设备
公开(公告)号：US20050016242A1
公开(公告)日：2005-01-27
申请号：US10497189
申请日：2002-09-30
申请人： Ryurou Kurahashi , Shinji Ueno , Takayoshi Komatsu , Masanori Takahashi , Akio Adachi , Shinji Takaoka
发明人： Ryurou Kurahashi , Shinji Ueno , Takayoshi Komatsu , Masanori Takahashi , Akio Adachi , Shinji Takaoka
IPC分类号： B21B1/26 , B21B13/14 , B21B45/02 , B21B13/08
CPC分类号： B21B1/26 , B21B13/142 , B21B45/0233 , B21B2267/06 , B21B2267/065 , B21B2275/10 , B21B2275/12
摘要： A continuous hot-rolling mill includes an upper rolling unit including a plurality of rolling stands, and a lower rolling unit including a plurality of rolling stands and disposed downstream with respect to the upper rolling unit in a workpiece passing direction in which a workpiece to be rolled is passed. The two or more rolling stands of the lower rolling unit are differential or very-small-diameter rolling stands. Each of the two or more differential or very-small-diameter rolling stands of the lower rolling unit is provided with a drive motor having a capacity greater than that of any one of drive motors included in the rolling stands disposed upstream the differential or very-small-diameter rolling stands. The continuous hot-rolling mill is suitable for producing hot-rolled, fine-grained steel sheets and is excellent in sheet passing abilities for preventing the steel sheet from meandering and for rolling the steel sheet in a desired shape.
摘要翻译：连续式热轧机包括具有多个轧制机架的上部轧制单元和具有多个轧制机架的下部轧制单元，并且在工件通过方向上相对于上部轧制单元的下游设置工件，轧制通过。下轧制单元的两个或多个轧机机座是差速或非常小直径的轧机机架。下轧制单元的两个或更多个差速或非常小直径的轧制机架中的每一个设置有驱动电动机，该驱动电动机的容量大于设置在差速器上方的滚动机架中的任何一个驱动电动机，小直径轧机机架。连续式热轧机适用于生产热轧，细粒度的钢板，并且具有优异的板材通过能力，用于防止钢板曲折和将钢板轧制成所需形状。

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