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    • 8. 发明申请
    • DESATURASE GENES, ENZYMES ENCODED THEREBY, AND USES THEREOF
    • 去饱和基因,编码的酶,及其用途
    • US20070124837A1
    • 2007-05-31
    • US11621446
    • 2007-01-09
    • Pradip MukerjiSuzette PereiraYung-Sheng Huang
    • Pradip MukerjiSuzette PereiraYung-Sheng Huang
    • A01H1/00C07H21/04
    • C12N9/0083C12P7/6427C12P7/6472
    • Disclosed are isolated polynucleotides encoding an omega-3 desaturase and a delta-12 desaturase, the enzymes encoded by the isolated polynucleotides, vectors containing the isolated polynucleotides, transgenic hosts that contain the isolated polynucleotides that express the enzymes encoded thereby, methods for producing the desaturase enzymes, and method of using the enzymes to make polyunsaturated fatty acids. The isolated polynucleotides are derived from a fungus, Saprolegnia diclina (ATCC 56851). In particular, omega-3-desaturase may be utilized, for example, in the conversion of arachidonic acid (AA) to eicosapentaenoic acid (EPA). Delta-12 desaturase may be used, for example, in the conversion of oleic acid (OA) to linoleic (LA). EPA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
    • 公开了编码ω-3去饱和酶和δ-12去饱和酶的分离的多核苷酸,由分离的多核苷酸编码的酶,含有分离的多核苷酸的载体,含有表达由其编码的酶的分离的多核苷酸的转基因宿主,用于产生去饱和酶的方法 酶和使用酶制备多不饱和脂肪酸的方法。 分离的多核苷酸衍生自真菌Saprolegnia diclina(ATCC 56851)。 特别地,ω-3-去饱和酶可用于例如花生四烯酸(AA)转化为二十碳五烯酸(EPA)。 Δ-12去饱和酶可以用于例如将油酸(OA)转化为亚油酸(LA)。 由此产生的EPA或多不饱和脂肪酸可以加入到药物组合物,营养组合物,动物饲料中,以及其它产品如化妆品中。