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1. 发明授权

US06354242B1 Methods for gamete production in birds 有权
标题翻译：鸟类配子生产方法
公开(公告)号：US06354242B1
公开(公告)日：2002-03-12
申请号：US09533141
申请日：2000-03-23
申请人： Samuel L. Pardue , James N. Petitte , Susan D'Costa
发明人： Samuel L. Pardue , James N. Petitte , Susan D'Costa
IPC分类号： A01K4500
CPC分类号： A01K67/02 , A01K45/00 , A01K45/007
摘要： A method for the production and collection of avian sperm comprises the steps of: providing primordial germ cells from a donor avian species; administering the primordial germ cells to a recipient avian species in ovo; incubating the recipient avian species to hatch; and then collecting sperm of the donor avian species from the recipient avian species. For example, the donor avian species may be a whooping crane, and the recipient avian species may be a sand hill crane. In another example, the donor avian species may be a turkey, and the recipient avian species may be a chicken.
摘要翻译：生产和收集禽精子的方法包括以下步骤：从供体鸟种中提供原始生殖细胞; 将原始生殖细胞在卵内施用于受体禽类物种; 孵化接受禽鸟种; 然后从受体禽类物种中收集供体禽类物种的精子。例如，供体鸟类可能是一个百日鹤，接受禽鸟种可能是沙丘起重机。在另一个例子中，供体鸟类物种可能是一只火鸡，而接受禽鸟种可能是一只鸡。

2. 发明授权

US06691638B2 Methods for gamete production in birds 失效
标题翻译：鸟类配子生产方法
公开(公告)号：US06691638B2
公开(公告)日：2004-02-17
申请号：US10074719
申请日：2002-02-12
申请人： Samuel Pardue , James Petitte , Susan D'Costa , Yonghong Song
发明人： Samuel Pardue , James Petitte , Susan D'Costa , Yonghong Song
IPC分类号： A01K4500
CPC分类号： A01K67/02 , A01K45/00 , A01K45/007
摘要： The present invention provides methods for the production of avian gametes, comprising reducing endogenous primordial germ cells (PGCs) in a recipient bird in ovo and introducing donor primordial germ cells from another bird into the recipient bird in ovo. The present invention further provides a method of increasing the ratio of male offspring from a female bird, comprising reducing endogenous PGCs in the female bird in ovo, introducing donor PGCs from another bird into the female bird in ovo, and incubating the female bird to hatch. The female bird is raised to sexual maturity and bred to produce a plurality of eggs having a higher proportion of male eggs than in the absence of the inventive methods. In some embodiments, the donor PGCs are from a different species than the recipient bird. In preferred embodiments, endogenous PGCs are reduced using busulfan.
摘要翻译：本发明提供了用于生产禽配子的方法，其包括在卵内减少受体鸟中的内源性原始生殖细胞（PGC），并将来自另一鸟的供体原始生殖细胞以卵子形式引入受体鸟。本发明还提供了一种增加雌性雌性雄性后代比例的方法，其包括在卵中减少雌鸟中的内源性PGC，将来自另一只鸟的供体PGC以卵子方式引入雌性鸟，并将雌性鸟孵化至孵化。将雌性的雌鸟升至性成熟期并繁殖以产生比没有本发明方法更高比例的雄性卵的多个卵。在一些实施方案中，供体PGC来自与受体鸟类不同的物种。在优选的实施方案中，使用白消安，还原内源性PGC。

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