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    • 8. 发明申请
    • Method of immobilizing a protein to a zeolite
    • 将蛋白质固定在沸石上的方法
    • US20060240567A1
    • 2006-10-26
    • US10534162
    • 2003-11-07
    • Stanley BrownRune WendelboSanne Nygaard
    • Stanley BrownRune WendelboSanne Nygaard
    • G01N33/558
    • C07K17/14C07K14/001C07K19/00C07K2319/00
    • The present invention relates to a polypeptide tag and a method using said polypeptide tag sequence for immobilizing a protein on a microporous material, said microporous material is selected from the group consisting of zeolite or similar solid surfaces whereby loss of activity of said protein is less than 10% of the initial activity prior to immobilization, the method comprising the steps of: 1. Selecting a polypeptide tag capable of binding to the surface, 2. Immobilizing said protein by the steps of: attaching said polypeptide tag to the protein, and binding said polypeptide tag to the solid surface where step (a) and (b) is performed simultaneously or sequentially and when performed sequentially, the order of step (a) and (b) is random, subject to the limitation that the polypeptide tag does not consist only of histidine residues.
    • 本发明涉及多肽标签和使用所述多肽标签序列将蛋白质固定在微孔材料上的方法,所述微孔材料选自沸石或类似的固体表面,由此所述蛋白质的活性丧失小于 10%的固定前的初始活性,所述方法包括以下步骤:1.选择能够结合表面的多肽标签; 2.通过以下步骤固定所述蛋白质:将所述多肽标签连接到蛋白质上,并结合 所述多肽标签到固体表面,其中步骤(a)和(b)同时或顺序地进行,并且当顺序进行时,步骤(a)和(b)的顺序是随机的,但受限于多肽标签不 仅由组氨酸残基组成。