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1. 发明申请

US20070108058A1 Compositions, methods, devices, and systems for nucleic acid fractionation 审中-公开
$Compositions, methods, devices, and systems for nucleic acid fractionation$
标题翻译：用于核酸分馏的组合物，方法，装置和系统
公开(公告)号：US20070108058A1
公开(公告)日：2007-05-17
申请号：US11373646
申请日：2006-03-10
申请人： Rick Conrad , Patricia Powers , Laith Vincent , Scott Hunicke-Smith
发明人： Rick Conrad , Patricia Powers , Laith Vincent , Scott Hunicke-Smith
IPC分类号： B01D35/06 , C02F1/40 , B03C5/02 , C02F11/00
CPC分类号： G01N27/44704 , C12N15/101 , G01N27/44747
摘要： The present disclosure provides methods, devices, systems and compositions for nucleic acid separation and/or purification. In some embodiments, nucleic acids from about 10 nucleotides to about 150 nucleotides may be separated and/or purified in seconds to minutes. A system for purifying a nucleic acid within seconds to minutes may include: a fractionator having a housing, a first electrode, a second electrode spaced away from the first electrode, and a lower buffer chamber proximal to the second electrode; and a pre-cast gel cartridge having an upper buffer chamber and an elongate polyacrylamide gel, wherein the upper buffer chamber is in fluid communication with one end of the polyacrylamide gel, the lower buffer chamber is in fluid communication with the other end of the elongate polyacrylamide gel, the first electrode is in electrical communication with the upper buffer chamber, and the second electrode is in electrical communication with the lower buffer chamber.
摘要翻译：本公开提供了用于核酸分离和/或纯化的方法，装置，系统和组合物。在一些实施方案中，约10个核苷酸至约150个核苷酸的核酸可在几秒至数分钟内分离和/或纯化。用于在数秒至数分钟内纯化核酸的系统可以包括：具有壳体的分馏器，第一电极，与第一电极间隔开的第二电极和靠近第二电极的下缓冲室; 和具有上缓冲室和细长聚丙烯酰胺凝胶的预制凝胶盒，其中上缓冲室与聚丙烯酰胺凝胶的一端流体连通，下缓冲室与细长的另一端流体连通聚丙烯酰胺凝胶，第一电极与上缓冲室电连通，第二电极与下缓冲室电连通。

2. 发明申请

US20100101954A1 Compositions, Methods, Devices, and Systems for Nucleic Acid Fractionation 审中-公开
标题翻译：用于核酸分馏的组合物，方法，装置和系统
公开(公告)号：US20100101954A1
公开(公告)日：2010-04-29
申请号：US12430279
申请日：2009-04-27
申请人： RICK CONRAD , PATRICIA K. POWERS , LAITH VINCENT , SCOTT HUNICKE-SMITH
发明人： RICK CONRAD , PATRICIA K. POWERS , LAITH VINCENT , SCOTT HUNICKE-SMITH
IPC分类号： C07K1/26
CPC分类号： G01N27/44704 , C12N15/101 , G01N27/44747
摘要： The present disclosure provides methods, devices, systems and compositions for nucleic acid separation and/or purification. In some embodiments, nucleic acids from about 10 nucleotides to about 150 nucleotides may be separated and/or purified in seconds to minutes. A system for purifying a nucleic acid within seconds to minutes may include: a fractionator having a housing, a first electrode, a second electrode spaced away from the first electrode, and a lower buffer chamber proximal to the second electrode; and a pre-cast gel cartridge having an upper buffer chamber and an elongate polyacrylamide gel, wherein the upper buffer chamber is in fluid communication with one end of the polyacrylamide gel, the lower buffer chamber is in fluid communication with the other end of the elongate polyacrylamide gel, the first electrode is in electrical communication with the upper buffer chamber, and the second electrode is in electrical communication with the lower buffer chamber.
摘要翻译：本公开提供了用于核酸分离和/或纯化的方法，装置，系统和组合物。在一些实施方案中，约10个核苷酸至约150个核苷酸的核酸可在几秒至数分钟内分离和/或纯化。用于在数秒至数分钟内纯化核酸的系统可以包括：具有壳体的分馏器，第一电极，与第一电极间隔开的第二电极和靠近第二电极的下缓冲室; 和具有上缓冲室和细长聚丙烯酰胺凝胶的预制凝胶盒，其中上缓冲室与聚丙烯酰胺凝胶的一端流体连通，下缓冲室与细长的另一端流体连通聚丙烯酰胺凝胶，第一电极与上缓冲室电连通，第二电极与下缓冲室电连通。

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