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1. 发明申请

US20100129937A1 METHOD FOR MEASURING HYALURONIC ACID USING HYALURONIC ACID BINDING PROTEIN 审中-公开
标题翻译：用羟丙酸结合蛋白测定羟丙酸的方法
公开(公告)号：US20100129937A1
公开(公告)日：2010-05-27
申请号：US12578576
申请日：2009-10-13
申请人： KYOICHI SUMIDA , KAZUNARI FUJIO , SHINZO KOBATAKE
发明人： KYOICHI SUMIDA , KAZUNARI FUJIO , SHINZO KOBATAKE
IPC分类号： G01N33/543
CPC分类号： G01N33/543 , G01N21/82 , G01N2400/40
摘要： An object of the present invention is to provide a method utilizing a carrier for measuring hyaluronic acid wherein deposition of carrier is less, a storage stability is good and an accuracy in measurement has same high degree as that performed by the conventional reagents, and a reagent kit for the same. Also, the present invention relates to “a method for measuring hyaluronic acid, comprising forming a hyaluronic acid/HABP complex by contacting hyaluronic acid in a sample with HABP, reacting said complex with an anti-HABP antibody-supported carrier, measuring optical change by agglutination product generated by said reaction and calculating the quantity of hyaluronic acid from the measured value” and “a reagent kit for the measurement of hyaluronic acid comprising a reagent comprising a hyaluronic acid binding protein and a reagent comprising an anti-hyaluronic acid binding protein antibody-supported carrier”.
摘要翻译：本发明的目的是提供一种利用载体测量透明质酸的方法，其中载体沉积少，储存稳定性好，测量精度与常规试剂相同程度高，试剂套件相同。另外，本发明涉及“透明质酸测定方法，其包括通过使样品中的透明质酸与HABP接触而形成透明质酸/ HABP复合物，使所述配合物与抗HABP抗体负载的载体反应，通过由所述反应产生的凝集产物和从测量值计算透明质酸的量“和”用于测量透明质酸的试剂盒，其包含透明质酸结合蛋白的试剂和包含抗透明质酸结合蛋白抗体的试剂支持的运营商“。

2. 发明申请

US20070259380A1 Method for Measuring Hyaluronic Acid Using Hyaluronic Acid Binding Protein 审中-公开
标题翻译：透明质酸结合蛋白测定透明质酸的方法
公开(公告)号：US20070259380A1
公开(公告)日：2007-11-08
申请号：US11597191
申请日：2005-05-10
申请人： Kyoichi Sumida , Kazunari Fujio , Shinzo Kobatake
发明人： Kyoichi Sumida , Kazunari Fujio , Shinzo Kobatake
IPC分类号： G01N33/53
CPC分类号： G01N33/543 , G01N21/82 , G01N2400/40
摘要： An object of the present invention is to provide a method utilizing a carrier for measuring hyaluronic acid wherein deposition of carrier is less, a storage stability is good and an accuracy in measurement has same high degree as that performed by the conventional reagents, and a reagent kit for the same. Also, the present invention relates to “a method for measuring hyaluronic acid, comprising forming a hyaluronic acid/HABP complex by contacting hyaluronic acid in a sample with HABP, reacting said complex with an anti-HABP antibody-supported carrier, measuring optical change by agglutination product generated by said reaction and calculating the quantity of hyaluronic acid from the measured value” and “a reagent kit for the measurement of hyaluronic acid comprising a reagent comprising a hyaluronic acid binding protein and a reagent comprising an anti-hyaluronic acid binding protein antibody-supported carrier”.
摘要翻译：本发明的目的是提供一种利用载体测量透明质酸的方法，其中载体沉积少，储存稳定性好，测量精度与常规试剂相同程度高，试剂套件相同。另外，本发明涉及“透明质酸测定方法，其包括通过使样品中的透明质酸与HABP接触而形成透明质酸/ HABP复合物，使所述配合物与抗HABP抗体负载的载体反应，通过由所述反应产生的凝集产物和从测量值计算透明质酸的量“和”用于测量透明质酸的试剂盒，其包含透明质酸结合蛋白的试剂和包含抗透明质酸结合蛋白抗体的试剂支持的运营商“。

3. 发明授权

US4295853A Method for measuring peroxidic substances 失效
标题翻译：过氧化物质测定方法
公开(公告)号：US4295853A
公开(公告)日：1981-10-20
申请号：US974148
申请日：1978-12-29
申请人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
发明人： Shizuka Kasahara , Kazuhiko Yamanishi , Toshiro Hanada , Kuniaki Tokuda , Shinzo Kobatake
IPC分类号： C07D231/48 , G01N31/22 , G01N33/52 , G01N33/92
CPC分类号： G01N33/52 , C07D231/48 , G01N31/228
摘要： Peroxidic substances in a sample, e.g. one or more peroxidic substances in fats and oils or in an organic material, lipoperoxides in a living sample, etc. can be determined, directly in a sample or after extracting the lipoperoxides from a living sample with a solvent, by contacting the peroxidic substances with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color, and measuring degree of coloring colorimetrically.
摘要翻译：样品中的过氧化物质，例如脂肪和油或有机材料中的一种或多种过氧化物质，活体样品中的脂肪过氧化物等可以直接在样品中或在用溶剂从活体样品中提取脂肪过氧化物之后，通过使过氧化物质与在由能够具有两个或多个化合价产生颜色的金属制成的金属化合物存在下的可氧化着色试剂，并以比色测定着色程度。

4. 发明授权

US5273908A Stabilizing method for immuno active substances immobilized on insoluble carrier and its use in preparation of reagent for measuring physiologically active substances 失效
标题翻译：固定在不溶性载体上的免疫活性物质的稳定化方法及其在制备用于测定生理活性物质的试剂中的用途
公开(公告)号：US5273908A
公开(公告)日：1993-12-28
申请号：US659476
申请日：1991-02-25
申请人： Yoshitsugu Sakata , Yoshitaka Hamaguchi , Motoo Goto , Shinzo Kobatake
发明人： Yoshitsugu Sakata , Yoshitaka Hamaguchi , Motoo Goto , Shinzo Kobatake
IPC分类号： G01N33/543 , G01N33/544 , G01N33/551
CPC分类号： G01N33/54393
摘要： An immuno active substance immobilized on a carrier and stabilized by immersing the carrier in a solution of at least one of sugars and proteins can be used for measuring a physiologically active substance even after stored for a long period of time. A preferred embodiment of the invention uses a synergistic mixture of a sugar and a protein as the stabilizing agents.
摘要翻译：固定在载体上并通过将载体浸渍在糖和蛋白质中的至少一种的溶液而稳定化的免疫活性物质即使在长时间储存后也可用于测量生理活性物质。本发明的优选实施方案使用糖和蛋白质的协同混合物作为稳定剂。

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