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1. 发明授权

US09932620B2 Methods, devices, and systems of detecting microorganisms 有权
公开(公告)号：US09932620B2
公开(公告)日：2018-04-03
申请号：US13537267
申请日：2012-06-29
申请人： Subramani Sellappan , Andrew Hearn , Salvatore Seminara
发明人： Subramani Sellappan , Andrew Hearn , Salvatore Seminara
IPC分类号： C12M1/34 , G01N21/76 , G01N1/34 , C12Q1/04 , G01N21/77 , G01N1/38 , G01N33/569 , G01N1/02 , G01N1/40
CPC分类号： C12Q1/04 , G01N1/38 , G01N21/76 , G01N21/77 , G01N33/56911 , G01N2001/028 , G01N2001/4061 , G01N2001/4088 , G01N2201/02
摘要： A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer.

2. 发明申请

US20050176034A1 Microfluidics apparatus and methods of use therefor 审中-公开
标题翻译：微流体装置及其使用方法
公开(公告)号：US20050176034A1
公开(公告)日：2005-08-11
申请号：US10992368
申请日：2004-11-18
申请人： John Peeters , Thomas Wiggins , Madhushree Ghosh , Lawrence Bottomley , Salvatore Seminara , Zhiyu Hu , Timothy Seeley , Sebastian Kossek
发明人： John Peeters , Thomas Wiggins , Madhushree Ghosh , Lawrence Bottomley , Salvatore Seminara , Zhiyu Hu , Timothy Seeley , Sebastian Kossek
IPC分类号： B01L3/00 , B81B3/00 , G01N1/00 , G01N27/447 , G01N33/53 , G01N33/543 , G01N37/00 , C12Q1/68 , C12M1/34
CPC分类号： G01N33/54366 , B01L3/5025 , B01L3/502738 , B01L2200/10 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/18 , B01L2400/0487 , B01L2400/0633 , Y10T436/11
摘要： A microfluidics device includes a plurality of interaction cells and fluid control means including i) means for providing to the interaction cells a preparation fluid, and ii) means for providing to the interaction cells a sample fluid, wherein each interaction cell receives a different sample fluid. A plurality of microcantilevers may be disposed in each of the interaction cells, wherein each of the plurality of microcantilevers configured to deflect in response to an interaction involving a component of the sample fluid.
摘要翻译：微流体装置包括多个相互作用单元和流体控制装置，其包括i）向相互作用的单元提供制备流体的装置，和ii）向相互作用的单元提供样品流体的装置，其中每个相互作用单元接收不同的样品流体。多个微悬臂梁可以布置在每个相互作用单元中，其中多个微悬臂梁中的每一个构造成响应于涉及样品流体的一个部件的相互作用而偏转。

3. 发明申请

US20120301907A1 METHODS, DEVICES, AND SYSTEMS OF DETECTING MICROORGANISMS 有权
标题翻译：检测微生物的方法，设备和系统
公开(公告)号：US20120301907A1
公开(公告)日：2012-11-29
申请号：US13537267
申请日：2012-06-29
申请人： SUBRAMANI SELLAPPAN , ANDREW HEARN , SALVATORE SEMINARA
发明人： SUBRAMANI SELLAPPAN , ANDREW HEARN , SALVATORE SEMINARA
IPC分类号： G01N21/76 , C12M1/34 , G01N1/34
CPC分类号： C12Q1/04 , G01N1/38 , G01N21/76 , G01N21/77 , G01N33/56911 , G01N2001/028 , G01N2001/4061 , G01N2001/4088 , G01N2201/02
摘要： A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical, cosmetic, personal care, pharmaceutical, or consumable good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer.
摘要翻译：从可能含有微生物（例如但不限于细菌，真菌，酵母，病毒等）的样品中分离和检测微生物的快速，灵敏的方法。该方法依赖于分离技术以将样品中的细胞与化学技术一起分离和浓缩，从而扩增来自低细胞数量的可检测信号的量，以提供检测微生物的快速和灵敏的方法。该检测方法可以利用：过滤装置; 离心装置; 一个系统; 拭子装置以及包含一个或多个装置和组件的试剂盒，以执行分离和检测可能含有微生物的样品中的微生物的本方法。样品可能是化学，化妆品，个人护理，药物或消耗品的原材料，在制品和/或成品状态，需要在装运到消费者之前对任何污染的微生物进行测试。

4. 发明申请

US20060121502A1 Microfluidics apparatus for cantilevers and methods of use therefor 审中-公开
标题翻译：用于悬臂的微流体装置及其使用方法
公开(公告)号：US20060121502A1
公开(公告)日：2006-06-08
申请号：US11254348
申请日：2005-10-20
申请人： Robert Cain , Paul Mirer , Salvatore Seminara , Timothy Seeley , Sebastian Kossek , Thomas Wiggins
发明人： Robert Cain , Paul Mirer , Salvatore Seminara , Timothy Seeley , Sebastian Kossek , Thomas Wiggins
IPC分类号： C12Q1/68 , G01N33/53 , G06F19/00 , C12M1/34
CPC分类号： G01N33/54373
摘要： A microfluidics device includes a plurality of interaction cells and fluid control means including i) means for providing to the interaction cells a preparation fluid, ii) means for providing to the interaction cells a sample fluid, wherein each interaction cell receives a different sample fluid, and iii) means for thermal control. A plurality of cantilevers may be disposed in each of the interaction cells, the cells or chambers formed by a cartridge bottom and top that form the device, wherein each of the plurality of cantilevers is configured to deflect in response to an interaction involving a component of the sample fluid. The cantilevers in each cell are attached to a reference plane that controls for environmental factors or non-analyte deflections.
摘要翻译：微流体装置包括多个相互作用单元和流体控制装置，其包括i）向相互作用的单元提供制备流体的装置，ii）向相互作用的单元提供样品流体的装置，其中每个相互作用单元接收不同的样品流体，和iii）用于热控制的装置。多个悬臂可以设置在每个相互作用单元中，由形成该装置的盒底部和顶部形成的单元或室，其中多个悬臂中的每一个被配置为响应于涉及一个样品液。每个电池中的悬臂连接到控制环境因素或非分析物偏转的参考平面。

5. 发明授权

US06926864B2 Microfluidics apparatus and methods for use thereof 失效
标题翻译：微流体装置及其使用方法
公开(公告)号：US06926864B2
公开(公告)日：2005-08-09
申请号：US10054760
申请日：2001-11-13
申请人： John P. Peeters , Thomas Wiggins , Madhushree Ghosh , Lawrence A. Bottomley , Salvatore Seminara , Zhiyu Hu , Timothy Seeley , Sebastian Kossek
发明人： John P. Peeters , Thomas Wiggins , Madhushree Ghosh , Lawrence A. Bottomley , Salvatore Seminara , Zhiyu Hu , Timothy Seeley , Sebastian Kossek
IPC分类号： B01L3/00 , B81B3/00 , G01N1/00 , G01N27/447 , G01N33/53 , G01N33/543 , G01N37/00 , G01N15/06 , G01N27/00 , G01N30/96 , G01N33/00 , G01N33/48
CPC分类号： G01N33/54366 , B01L3/5025 , B01L3/502738 , B01L2200/10 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/18 , B01L2400/0487 , B01L2400/0633 , Y10T436/11
摘要： A microfluidics device includes a plurality of interaction cells and fluid control means including i) means for providing to the interaction cells a preparation fluid, and ii) means for providing to the interaction cells a sample fluid, wherein each interaction cell receives a different sample fluid. A plurality of microcantilevers may be disposed in each of the interaction cells, wherein each of the plurality of microcantilevers configured to deflect in response to an interaction involving a component of the sample fluid.
摘要翻译：微流体装置包括多个相互作用单元和流体控制装置，其包括i）向相互作用的单元提供制备流体的装置，和ii）向相互作用的单元提供样品流体的装置，其中每个相互作用单元接收不同的样品流体。多个微悬臂梁可以布置在每个相互作用单元中，其中多个微悬臂梁中的每一个构造成响应于涉及样品流体的一个部件的相互作用而偏转。

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