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1. 发明授权

US07741461B2 Nucleic acid probes and methods for detecting clinically important fungal pathogens 有权
标题翻译：核酸探针和检测临床重要真菌病原体的方法
公开(公告)号：US07741461B2
公开(公告)日：2010-06-22
申请号：US10967254
申请日：2004-10-19
申请人： Terry Smith , Majella Maher , Cara Martin , Geert Jannes , Rudi Rossau , Marjo Van der Weide
发明人： Terry Smith , Majella Maher , Cara Martin , Geert Jannes , Rudi Rossau , Marjo Van der Weide
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6895
摘要： The current invention relates to the field of detection and identification of clinically important fungi. More particularly, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cryptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.
摘要翻译：本发明涉及临床重要真菌的检测和鉴定领域。更具体地说，本发明涉及源自rDNA的内部转录间隔区（ITS）区域的物种特异性探针，用于检测真菌种类，例如白色念珠菌，副伤寒假念杆菌，热带假丝酵母，假丝酵母，假丝酵母，光滑假丝酵母，念珠菌都柏林，曲霉，黄曲霉，构巢曲霉，烟曲霉，新型隐球菌和卡氏肺囊虫，以及使用所述探针的方法。

2. 发明申请

US20100120121A1 Process for typing of HCV isolates 失效
标题翻译： HCV分离株的分型方法
公开(公告)号：US20100120121A1
公开(公告)日：2010-05-13
申请号：US11826099
申请日：2007-07-12
申请人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
发明人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
IPC分类号： C12N7/01 , C07H21/00
CPC分类号： C12Q1/707
摘要： The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.
摘要翻译：本发明涉及用于对先前鉴定为HCV阳性的生物样品中存在的任何HCV分离物进行基因分型的方法，以及根据与其它HCV分离株的同源性百分比对所述分离物进行分类的方法，包括以下步骤：如果需要的话，核糖核苷酸或脱氧核糖核苷酸可以在合适的变性下与至少一个约10至约40个核苷酸的探针接触，所述探针易于与位置延伸的结构域延伸的区域杂交。 291到由其cDNA序列表示的HCV分离物之一的5'非翻译区的-66位的核苷酸，其中所述编号的位置开始于编码HCV多蛋白的开放阅读框的第一个ATG密码子，或与所述探针与上述探针互补，检测可能在所述探针和核苷酸序列之间形成的复合物 HCV分离株被鉴定。

3. 发明申请

US20100099081A1 Method for Typing HLA Alleles 失效
标题翻译：分类HLA等位基因的方法
公开(公告)号：US20100099081A1
公开(公告)日：2010-04-22
申请号：US12102281
申请日：2008-04-14
申请人： Ilse de Canck , Guy Mersch , Rudi Rossau
发明人： Ilse de Canck , Guy Mersch , Rudi Rossau
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C07K14/70539 , C12Q2600/156 , C12Q2600/16
摘要： The present invention relates to the typing of HLA alleles. The sequence of exon 2 and exon 3 of the alleles HLA-B*3913, HLA-B*1406 and HLA-B*51 new and of exon 2 of the alleles HLA-DRB1*0820, HLA-DRB1*04 new and HLA-DRB4*01 new are disclosed. The present invention relates to methods for typing of said alleles. According to a preferred embodiment, said typing comprises the following steps: i) amplifying a relevant fragment of said alleles using at least one suitable pair of primers; ii) hybridizing the amplification product of step i) to at least one probe that specifically hybridizes to a target region comprising one or more polymorphic nucleotides in said relevant fragment; iii) determining from the result of step ii) the absence or presence of said alleles in the sample. The present invention further provides primers and probes to be used in said methods for typing. A diagnostic kit comprising said primers and probes is also part of the present invention.
摘要翻译：本发明涉及HLA等位基因的分型。等位基因HLA-DRB1 * 0820，HLA-DRB1 * 04新和HLA的等位基因HLA-B * 3913，HLA-B * 1406和HLA-B * 51新和外显子2的外显子2和外显子3的序列 -DRB4 * 01新发布。本发明涉及所述等位基因的分型方法。根据优选实施方案，所述分型包括以下步骤：i）使用至少一对合适的引物扩增所述等位基因的相关片段; ii）将步骤i）的扩增产物杂交至至少一种与所述相关片段中包含一个或多个多核苷酸的靶区域特异性杂交的探针; iii）从步骤ii）的结果确定样品中不存在或存在所述等位基因。本发明还提供了用于所述打字方法的引物和探针。包括所述引物和探针的诊断试剂盒也是本发明的一部分。

4. 发明申请

US20050175990A1 Method for typing and detecting HBV 有权
标题翻译： HBV的分型和检测方法
公开(公告)号：US20050175990A1
公开(公告)日：2005-08-11
申请号：US10606879
申请日：2003-06-27
申请人： Lieven Stuyver , Rudi Rossau , Geert Maertens
发明人： Lieven Stuyver , Rudi Rossau , Geert Maertens
IPC分类号： C12N15/36 , C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/706
摘要： The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG. 1, and with said probes being applied to known locations on a solid support and with said probes being capable of hybridizing to the polynucleic acids of the sample under the same hybridization and wash conditions, or with said probes hybridizing specifically with a sequence complementary to any of said target sequences, or a sequence where T of said target sequence is replaced by U; and detecting the hybrids formed; and inferring the HBV genotype and/or mutants present in said sample from the differential hybridization signal(s) obtained. The invention further relates to sets of nucleotide probes and possibly primers useful in said methods as well as to their use in a method for typing and/or detecting HBV and to assay kits using the same.
摘要翻译：本发明涉及用于生物样品中HBV的检测和/或遗传分析的方法，包括将所述样品的多核酸与至少两个核苷酸探针的组合杂交，所述组合特异性与选择的突变靶序列杂交从HBV RT pol基因区域和/或选自HBV preCore区域的突变靶序列和/或选自HBV的HBsAg区域和/或HBV基因型特异性靶序列的突变靶序列，其中所述靶序列选自图1。 1，并且所述探针被施加到固体支持物上的已知位置，并且所述探针能够在相同的杂交和洗涤条件下与样品的多核酸杂交，或者与所述探针特异性地与任何与任何或所述靶序列的T被U代替的序列; 并检测形成的杂种; 并从所获得的差异杂交信号中推断存在于所述样品中的HBV基因型和/或突变体。本发明还涉及可用于所述方法的核苷酸探针和可能的引物的集合，以及它们在用于分型和/或检测HBV的方法中的用途以及使用其的测定试剂盒。

5. 发明授权

US06656689B2 Hybridization probes derived from the spacer region between the 16S and 23S rRNA genes for the detection of non-viral microorganisms 失效
公开(公告)号：US06656689B2
公开(公告)日：2003-12-02
申请号：US09863086
申请日：2001-05-22
申请人： Rudi Rossau , Hugo Van Heuverswyn
发明人： Rudi Rossau , Hugo Van Heuverswyn
IPC分类号： C12Q168
CPC分类号： C12Q1/6888 , C12Q1/689
摘要： The invention relates to a probe consisting of at least about 15 nucleotides from the spacer region between rRNA genes of a non-viral organism, particularly prokaryotic organism and more particularly bacteria, and preferably from about 15 nucleotides to about the maximum number of nucleotides of the spacer region and more preferably from about 15 to about 100 nucleotides to be used for the detection of non-viral microorganisms.

6. 发明授权

US5846704A Process for typing of HCV isolates 失效
标题翻译： HCV分离株的分型方法
公开(公告)号：US5846704A
公开(公告)日：1998-12-08
申请号：US256568
申请日：1994-07-18
申请人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
发明人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
IPC分类号： C12N15/09 , C07H21/04 , C12N15/40 , C12Q1/02 , C12Q1/68 , C12Q1/70 , C12N9/34
CPC分类号： C12Q1/707 , C12Q1/6834
摘要： A method of genotyping of HCV isolates using probes targeting sequences from the 5- untranslated region of HCV.
摘要翻译： PCT No.PCT / EP93 / 03325 Sec。 371日期：1994年7月18日 102（e）1994年7月18日PCT 1993年11月26日PCT公布。公开号WO94 / 12670 日期1994年6月9日使用来自HCV的5-非翻译区的序列的探针对HCV分离株进行基因分型的方法。

7. 发明授权

US08859203B2 Method for typing and detecting HBV 有权
公开(公告)号：US08859203B2
公开(公告)日：2014-10-14
申请号：US10606879
申请日：2003-06-27
申请人： Lieven Stuyver , Rudi Rossau , Geert Maertens
发明人： Lieven Stuyver , Rudi Rossau , Geert Maertens
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/04
CPC分类号： C12Q1/706
摘要： The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG. 1, and with said probes being applied to known locations on a solid support and with said probes being capable of hybridizing to the polynucleic acids of the sample under the same hybridization and wash conditions, or with said probes hybridizing specifically with a sequence complementary to any of said target sequences, or a sequence where T of said target sequence is replaced by U; and detecting the hybrids formed; and inferring the HBV genotype and/or mutants present in said sample from the differential hybridization signal(s) obtained. The invention further relates to sets of nucleotide probes and possibly primers useful in said methods as well as to their use in a method for typing and/or detecting HBV and to assay kits using the same.

8. 发明授权

US08835106B2 Method for typing and detecting HBV 有权
标题翻译： HBV的分型和检测方法
公开(公告)号：US08835106B2
公开(公告)日：2014-09-16
申请号：US11802328
申请日：2007-05-22
申请人： Lieven Stuyver , Rudi Rossau , Geert Maertens
发明人： Lieven Stuyver , Rudi Rossau , Geert Maertens
IPC分类号： C12Q1/70 , C07H21/04
CPC分类号： C12Q1/706
摘要： The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG. 1, and with said probes being applied to known locations on a solid support and with said probes being capable of hybridizing to the polynucleic acids of the sample under the same hybridization and wash conditions, or with said probes hybridizing specifically with a sequence complementary to any of said target sequences, or a sequence where T of said target sequence is replaced by U; and detecting the hybrids formed; and inferring the HBV genotype and/or mutants present in said sample from the differential hybridization signal(s) obtained. The invention further relates to sets of nucleotide probes and possibly primers useful in said methods as well as to their use in a method for typing and/or detecting HBV and to assay kits using the same.
摘要翻译：本发明涉及用于生物样品中HBV的检测和/或遗传分析的方法，包括将所述样品的多核酸与至少两个核苷酸探针的组合杂交，所述组合特异性与选择的突变靶序列杂交从HBV RT pol基因区域和/或选自HBV preCore区域的突变靶序列和/或选自HBV的HBsAg区域和/或HBV基因型特异性靶序列的突变靶序列，其中所述靶序列选自图1。 1，并且所述探针被施加到固体支持物上的已知位置，并且所述探针能够在相同的杂交和洗涤条件下与样品的多核酸杂交，或者与所述探针特异性地与任何与任何或所述靶序列的T被U代替的序列; 并检测形成的杂种; 并从所获得的差异杂交信号中推断存在于所述样品中的HBV基因型和/或突变体。本发明还涉及可用于所述方法的核苷酸探针和可能的引物的集合，以及它们在用于分型和/或检测HBV的方法中的用途以及使用其的测定试剂盒。

9. 发明授权

US07258982B2 Process for typing of HCV isolates 失效
标题翻译： HCV分离株的分型方法
公开(公告)号：US07258982B2
公开(公告)日：2007-08-21
申请号：US10822711
申请日：2004-04-13
申请人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
发明人： Geert Maertens , Lieven Stuyver , Rudi Rossau , Hugo Van Heuverswyn
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/707 , C12Q1/6834 , C12Q2563/131 , C12Q2545/101 , C12Q2525/101 , C12Q2531/113
摘要： The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes, detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.
摘要翻译：本发明涉及用于对先前鉴定为HCV阳性的生物样品中存在的任何HCV分离物进行基因分型的方法，以及根据与其它HCV分离株的同源性百分比对所述分离物进行分类的方法，包括以下步骤：如果需要的话，核糖核苷酸或脱氧核糖核苷酸可以在合适的变性下与至少一个约10至约40个核苷酸的探针接触，所述探针易于与位置延伸的结构域延伸的区域杂交。 291到由其cDNA序列表示的HCV分离物之一的5'非翻译区的-66位的核苷酸，其中所述编号的位置开始于编码HCV多蛋白的开放阅读框的第一个ATG密码子，或与所述探针与上述定义的探针互补，检测可能形成在所述探针与H的核苷酸序列之间的复合物 CV分离鉴定。

10. 发明授权

US06811978B2 Detection and identification of pseudomonas species using the 16S-23S rRNA spacer 失效
标题翻译：使用16S-23S rRNA间隔区检测和鉴定假单胞菌
公开(公告)号：US06811978B2
公开(公告)日：2004-11-02
申请号：US09931486
申请日：2001-08-17
申请人： Geert Jannes , Rudi Rossau , Hugo Van Heuverswyn
发明人： Geert Jannes , Rudi Rossau , Hugo Van Heuverswyn
IPC分类号： C12Q168
CPC分类号： C12Q1/689 , C12Q1/6888 , C12Q2600/16
摘要： The present invention relates to 16S-23S rRNA spacer sequences from Pseudomonas species and their use in a method for detection and/or identification of Pseudomonas species. The invention further it relates to a method for detection and identification of at least one Pseudomonas species, or for the simultaneous detection of several Pseudomonas species in a sample, involving the steps of: (i) optionally releasing, isolating and/or concentrating the polynucleic acids present in the sample; (ii) optionally amplifying the 16S-23S rRNA spacer region, or a part thereof, with at least one primer pair; (iii) detecting the presence of a 16S-23S rRNA spacer sequence; and (iv) identifying the Pseudomonas species present in the sample from the nucleic acid(s) detected in the sample.

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