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1. 发明授权

US07223566B2 Methods for production of proteins 有权
公开(公告)号：US07223566B2
公开(公告)日：2007-05-29
申请号：US10728766
申请日：2003-12-08
申请人： Deb K Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb K Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/04 , C12P21/06 , C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

2. 发明授权

US08519099B2 Methods for production of proteins 有权
标题翻译：生产蛋白质的方法
公开(公告)号：US08519099B2
公开(公告)日：2013-08-27
申请号：US13006354
申请日：2011-01-13
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

3. 发明授权

US08389681B2 Methods for production of proteins 有权
公开(公告)号：US08389681B2
公开(公告)日：2013-03-05
申请号：US11618725
申请日：2006-12-29
申请人： Deb K. Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb K. Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

4. 发明申请

US20110108420A1 METHODS FOR PRODUCTION OF PROTEINS 有权
标题翻译：生产蛋白质的方法
公开(公告)号：US20110108420A1
公开(公告)日：2011-05-12
申请号：US13006354
申请日：2011-01-13
申请人： DEB K. CHATTERJEE , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： DEB K. CHATTERJEE , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： B01D57/02 , C07K14/195 , C12N9/96 , C07K14/435 , C07K14/57 , C07K14/245
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

5. 发明授权

US06703484B2 Methods for production of proteins 失效
公开(公告)号：US06703484B2
公开(公告)日：2004-03-09
申请号：US09004068
申请日：1998-01-08
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K100
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

6. 发明申请

US20060286633A1 Methods for production of proteins 有权
公开(公告)号：US20060286633A1
公开(公告)日：2006-12-21
申请号：US11329401
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47 , C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

7. 发明申请

US20060269992A1 Methods of production of proteins 有权
标题翻译：蛋白质生产方法
公开(公告)号：US20060269992A1
公开(公告)日：2006-11-30
申请号：US11329418
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

8. 发明授权

US08012715B2 Methods for production of proteins 有权
标题翻译：生产蛋白质的方法
公开(公告)号：US08012715B2
公开(公告)日：2011-09-06
申请号：US11618723
申请日：2006-12-29
申请人： Deb K. Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb K. Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K1/00 , C12P21/04
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

9. 发明申请

US20070190606A1 Methods for Production of Proteins 有权
标题翻译：蛋白质生产方法
公开(公告)号：US20070190606A1
公开(公告)日：2007-08-16
申请号：US11618723
申请日：2006-12-29
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C12N9/02 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子梯度的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。

10. 发明申请

US20070184527A1 Methods for Production of Proteins 有权
公开(公告)号：US20070184527A1
公开(公告)日：2007-08-09
申请号：US11618725
申请日：2006-12-29
申请人： Deb CHATTERJEE , Mary LONGO , Elizabeth FLYNN , Robert OBERFELDER
发明人： Deb CHATTERJEE , Mary LONGO , Elizabeth FLYNN , Robert OBERFELDER
IPC分类号： C12P21/06 , C12N9/02 , C07H21/04 , C12N15/74 , C12N1/21
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

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