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    • 2. 发明授权
    • Molecular beacons
    • 分子信标
    • US07671184B2
    • 2010-03-02
    • US11575875
    • 2005-09-22
    • Robert HaenerSimon Langenegger
    • Robert HaenerSimon Langenegger
    • C07H21/02C07H21/04C12Q1/68B01L3/00C07H21/00
    • C12Q1/6816C12Q2563/107C12Q2525/301
    • The invention relates to a molecular beacon in the form of a hairpin oligonucleotide or oligonucleotide analogue comprising a first nucleotide sequence containing two or more aromatic or heteroaromatic ring systems P able to form an excimer or exciplex; a second sequence (the loop) consisting of an oligonucleotide probe able to hybridise with a target polynucleotide; and a third sequence containing one or more aromatic or heteroaromatic ring systems X, wherein at least one aromatic ring system X interacts with two aromatic ring systems P of the first sequence inhibiting excimer or exciplex formation. The invention further relates to a method for detecting the presence of a target polynucleotide using such a molecular beacon, and to a kit comprising a molecular beacon of the invention for use in this method.
    • 本发明涉及发夹寡核苷酸或寡核苷酸类似物形式的分子信标,其包含能够形成准分子或激酶复合物的含有两个或多个芳族或杂芳族环系统P的第一核苷酸序列; 由能够与靶多核苷酸杂交的寡核苷酸探针组成的第二序列(环) 和含有一个或多个芳族或杂芳族环系X的第三序列,其中至少一个芳族环系统X与第一序列的两个芳环系统P相互作用,抑制受激准分子或激元形成。 本发明还涉及使用这种分子信标检测靶多核苷酸的存在的方法,以及包含用于该方法的本发明的分子信标的试剂盒。