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    • 7. 发明授权
    • Introduction of modifying agents into skin by electroporation
    • 通过电穿孔将改性剂引入皮肤
    • US5911223A
    • 1999-06-15
    • US695367
    • 1996-08-09
    • James C. WeaverTani ChenChristopher CullanderRichard GuyRobert S. LangerThomas E. ZewertUwe PliquettRita VanbeverMark R. Prausnitz
    • James C. WeaverTani ChenChristopher CullanderRichard GuyRobert S. LangerThomas E. ZewertUwe PliquettRita VanbeverMark R. Prausnitz
    • A61B17/00A61N1/32A61B19/00
    • A61N1/0424A61N1/325A61B2017/00765A61N1/0428Y10S607/901
    • A method of modifying epidermis for transport of a material by electroporation includes applying to epidermis an agent that, upon entry into the epidermis, will modify the epidermis to thereby cause and altered rate of transport of a material across the epidermis. Typically, the altered rate will be an increased rate of transport. The epidermis is electroporated, whereby at least a portion of the modifying agent enters the electroporated epidermis, thereby modifying the epidermis to cause an altered rate of transport of a material across the epidermis. In another embodiment, the modifying agent can modify the epidermis to enable measurement and/or monitoring of physiological conditions or change within or beneath the epidermis. The modifying agents can also be employed to facilitate discharge of fluids from within an organism, such as by providing pathways for discharge of fluids from a tumor. Examples of modifying agents include: oxidizing agents; reducing agents; particles, such as optical indicator beads or beads that include drugs to be released into tissue; electrically-charged particles or molecules; etc. Materials that can be transported by the method of the invention include, for example, proteins, nucleic acids, electrically charged molecules or particles, microorganisms suitable for immunization, etc. Also, tissues other than skin can be employed in the method of the invention.
    • 通过电穿孔来改变用于运输材料的表皮的方法包括向表皮施加一种在进入表皮时将改变表皮从而引起和改变材料穿过表皮的运输速率的试剂。 通常,改变率将是增加的运输速度。 表皮被电穿孔,由此至少一部分改性剂进入电穿孔表皮,从而改变表皮,导致材料穿过表皮的转运速率。 在另一个实施方案中,修饰剂可以修饰表皮以使得能够测量和/或监测生理条件或在表皮内或下表面的变化。 还可以使用改性剂来促进从生物体内排出流体,例如通过提供从肿瘤排出流体的途径。 改性剂的实例包括:氧化剂; 还原剂; 颗粒,例如包括要释放到组织中的药物的光学指示剂珠粒或珠粒; 带电粒子或分子; 可以通过本发明的方法传输的材料包括例如蛋白质,核酸,带电分子或颗粒,适合免疫的微生物等。另外,皮肤以外的组织可以用于 发明。