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1. 发明授权

US09150936B2 Conditioning of biomass for improved C5/C6 sugar release prior to fermentation 有权
标题翻译：调节发酵前改善C5 / C6糖释放的生物量
公开(公告)号：US09150936B2
公开(公告)日：2015-10-06
申请号：US13547508
申请日：2012-07-12
申请人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
发明人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
IPC分类号： C13K13/00 , C13K1/02
CPC分类号： C13K1/02 , C12P2201/00
摘要： Disclosed is an improvement in a conventional process for C5 and C6 sugar recovery from lignocellulosic biomass for fermentation to ethanol, which process including the conventional steps of pretreatment of the biomass with steam at elevated temperature and pressure, collection of C5 sugars from hemicellulose breakdown, cellulose hydrolysis and collection of C6 sugars from cellulose breakdown. The improvement includes conditioning of the biomass prior to the pretreatment step by heating the biomass with steam for a time period between 5 minutes to 60 minutes to achieve a steam treated biomass having a temperature of about 80 to 100° C.; and adjusting a moisture content of the steam treated biomass to about 45% to 80%. An increased recovery of C5 and C6 sugars is achieved compared to the conventional process.
摘要翻译：公开了用于发酵到乙醇的木质纤维素生物质的C5和C6糖回收的常规方法的改进，该方法包括在高温和高压下用蒸汽预处理生物质的常规步骤，从半纤维素分解中收集C5糖，从纤维素分解中收集C5糖从纤维素分解中水解和收集C6糖。改进包括在预处理步骤之前通过用蒸汽加热生物质在5分钟至60分钟之间的时间段来调节生物质，以实现温度为约80至100℃的经蒸汽处理的生物质; 并将蒸汽处理的生物质的水分含量调节至约45％至80％。与常规方法相比，实现了C5和C6糖的增加的恢复。

2. 发明申请

US20130029406A1 TWO STAGE CONTINUOUS PRE-TREATMENT OF LIGNOCELLULOSIC BIOMASS 有权
标题翻译：两阶段连续预处理细菌生物量
公开(公告)号：US20130029406A1
公开(公告)日：2013-01-31
申请号：US13554601
申请日：2012-07-20
申请人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
发明人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
IPC分类号： C12S3/02 , C08B37/00
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16 , Y02E50/343
摘要： Disclosed is a method of pretreating biomass in two pretreatment stages as part of a biofuel production process. The first stage pretreatment is carried out by heating the biomass to a first stage temperature of 140° C. to 180° C. for a first stage time of 30 minutes to 2 hours at a first stage pressure of 105 to 150 psig; and the second stage is carried out by heating the biomass to a second stage temperature of 190° C. to 210° C. for a second stage time of 2 to 10 minutes at a second stage pressure of 167 to 262 psig. The biomass may be initially conditioned prior to the first pretreatment stage by atmospheric steam heating and adjusting the moisture content of the biomass. Hemicellulose and inhibitors (inhibitory compounds) to downstream hydrolysis and fermentation are preferably removed between the first and second pretreating stages, more preferably after each pretreatment stage.
摘要翻译：公开了在两个预处理阶段中预处理生物质的方法，作为生物燃料生产过程的一部分。第一阶段预处理通过在105至150psig的第一阶段压力下将生物质加热至140℃至180℃的第一阶段温度进行30分钟至2小时的第一阶段时间; 并且第二阶段通过在167-226psig的第二阶段压力下将生物质加热至190℃至210℃的第二阶段温度进行2至10分钟的第二阶段时间。生物质可以在第一预处理阶段之前通过大气蒸汽加热和调节生物量的水分含量进行初始调节。优选在第一和第二预处理阶段之间去除下游水解和发酵的半纤维素和抑制剂（抑制化合物），更优选在每个预处理阶段之后。

3. 发明申请

US20100313882A1 FRACTIONATION OF BIOMASS FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION 审中-公开
标题翻译：生物质的分解用于纤维素乙醇和化学生产
公开(公告)号：US20100313882A1
公开(公告)日：2010-12-16
申请号：US12766599
申请日：2010-04-23
申请人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
发明人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
IPC分类号： C13K1/02
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16
摘要： A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.
摘要翻译：定义了一种用于玉米芯和低木质素木质纤维素生物质的连续蒸汽预处理和分馏以产生对酶水解敏感的浓缩纤维素固体物流的方法。通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。生产用于发酵的生物燃料的纤维素衍生的葡萄糖。回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

4. 发明申请

US20050245443A1 Bacteria strain and bacteriocin produced therefrom 有权
标题翻译：由此产生的细菌菌株和细菌素
公开(公告)号：US20050245443A1
公开(公告)日：2005-11-03
申请号：US10834886
申请日：2004-04-30
申请人： Ismail Fliss , Michel Desbiens , Christophe Lacroix , Imane Tahiri , Regis-Olivier Benech , Ehab Kheadr
发明人： Ismail Fliss , Michel Desbiens , Christophe Lacroix , Imane Tahiri , Regis-Olivier Benech , Ehab Kheadr
IPC分类号： A61K38/00 , A61K38/16 , A61P31/04 , C07K14/195 , C07K14/335 , C12N1/20 , C12N15/31 , C12P21/02
CPC分类号： C07K14/335 , A61K38/00
摘要： The present invention concerns an isolated bacteriocin, Divergicin M35, derived form Carnobacterium divergens M35. It also concerns a composition comprising an effective amount of Divergicin M35 and the use of that bacteriocin to kill or limit the proliferation of a microorganism.
摘要翻译：本发明涉及分离的细菌素Divergicin M35，衍生自分枝杆菌M35。它还涉及一种包含有效量的分枝杆菌素M35的组合物，以及使用该细菌素来杀死或限制微生物的增殖。

5. 发明申请

US20130014749A1 CONDITIONING OF BIOMASS FOR IMPROVED C5/C6 SUGAR RELEASE PRIOR TO FERMENTATION 有权
标题翻译：生物量调节改善C5 / C6蔗糖发酵前的发酵
公开(公告)号：US20130014749A1
公开(公告)日：2013-01-17
申请号：US13547508
申请日：2012-07-12
申请人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
发明人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
IPC分类号： C13K13/00
CPC分类号： C13K1/02 , C12P2201/00
摘要： Disclosed is an improvement in a conventional process for C5 and C6 sugar recovery from lignocellulosic biomass for fermentation to ethanol, which process including the conventional steps of pretreatment of the biomass with steam at elevated temperature and pressure, collection of C5 sugars from hemicellulose breakdown, cellulose hydrolysis and collection of C6 sugars from cellulose breakdown. The improvement includes conditioning of the biomass prior to the pretreatment step by heating the biomass with steam for a time period between 5 minutes to 60 minutes to achieve a steam treated biomass having a temperature of about 80 to 100°C.; and adjusting a moisture content of the steam treated biomass to about 45% to 80%. An increased recovery of C5 and C6 sugars is achieved compared to the conventional process.
摘要翻译：公开了用于发酵到乙醇的木质纤维素生物质的C5和C6糖回收的常规方法的改进，该方法包括在高温和高压下用蒸汽预处理生物质的常规步骤，从半纤维素分解中收集C5糖，从纤维素分解中收集C5糖从纤维素分解中水解和收集C6糖。改进包括在预处理步骤之前通过用蒸汽加热生物质在5分钟至60分钟之间的时间段来调节生物质，以实现温度为约80至100℃的经蒸汽处理的生物质; 并将蒸汽处理的生物质的水分含量调节至约45％至80％。与常规方法相比，实现了C5和C6糖的增加的恢复。

6. 发明申请

US20120329116A1 PRETREATMENT OF LIGNOCELLULOSIC BIOMASS THROUGH REMOVAL OF INHIBITORY COMPOUNDS 审中-公开
标题翻译：通过去除抑制性化合物预处理细菌生物量
公开(公告)号：US20120329116A1
公开(公告)日：2012-12-27
申请号：US13555645
申请日：2012-07-23
申请人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
发明人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
IPC分类号： D21B1/36 , C12P7/14
CPC分类号： D21B1/36 , C12P2201/00 , D21C1/02 , D21C5/005 , D21C11/0007
摘要： A process for the pretreatment of lignocellulosic biomass is disclosed, which includes the steps of pretreating the lignocellulosic biomass to hydrolyze and solubilize hemicelluloses in the biomass; explosively decomposing the biomass into fibers; and extracting from the resulting solids fraction a liquefied portion of the lignocellulosic biomass before or after explosive decomposition. This removes compounds from the lignocellulosic biomass which are inhibitory to enzymatic cellulose hydrolysis and sugar fermentation to ethanol. For improved economy, the inhibitory compounds are not completely removed. The extraction step is controlled on the basis of the xylose equivalent content in the reaction mixture and the extracting step is discontinued once a xylose equivalent content of 4-8% w/w of xylose in the dry matter of the solids fraction is achieved. This most economically balances the practical need for inhibitory compound removal with the economical need to minimize the costs of the overall ethanol production process.
摘要翻译：公开了一种用于木质纤维素生物质的预处理的方法，其包括预处理木质纤维素生物质以在生物质中水解和增溶半纤维素的步骤; 将生物质爆炸分解成纤维; 并从所得固体部分提取在爆炸性分解之前或之后的木质纤维素生物质的液化部分。这从木质纤维素生物质中除去抑制酶纤维素水解和糖发酵成乙醇的化合物。为了改善经济性，抑制性化合物不能完全去除。提取步骤基于反应混合物中的木糖当量含量进行控制，一旦实现固体部分的干物质中木糖当量含量为4-8％w / w的木糖，就停止萃取步骤。这最经济地平衡了抑制化合物去除的实际需要与经济需要最小化整个乙醇生产过程的成本。

7. 发明授权

US08287651B2 Cellulose pretreatment process 有权
标题翻译：纤维素预处理工艺
公开(公告)号：US08287651B2
公开(公告)日：2012-10-16
申请号：US12560843
申请日：2009-09-16
申请人： Robert Benson , Chris Bradt , Regis-Olivier Benech , Richard Romeo Lehoux
发明人： Robert Benson , Chris Bradt , Regis-Olivier Benech , Richard Romeo Lehoux
IPC分类号： C13K1/02
CPC分类号： C08H8/00 , B09B3/0091 , C10G1/02 , C10G2300/1014 , C10G2300/4006 , C10G2300/4012 , C10G2300/807 , C10L1/02 , C12P2201/00 , Y02E50/16 , Y02P30/20 , Y10T137/0324
摘要： A process for the production of ethanol from biomass is disclosed. The process relates to the pretreatment of corncob biomass and includes the steps of exposing corncob to steam in a reaction vessel at an elevated temperature and reaction pressure for a preselected exposure time. Volatile compounds are continuously vented during pretreatment. The reaction pressure is released for explosive decomposition of the corncob biomass into fibrous solids and condensate. The exposure is preferably carried out at a temperature of 190-210° C. and a pressure of 190 psig to 275 psig and an exposure time of 3 to 10 minutes.
摘要翻译：公开了一种从生物质生产乙醇的方法。该方法涉及玉米芯生物质的预处理，包括以下步骤：将玉米芯在高温和反应压力下在反应容器中暴露于蒸汽中以进行预选的曝光时间。挥发性化合物在预处理过程中持续排出。释放反应压力以将玉米芯生物质炸药分解成纤维状固体和冷凝物。曝光优选在190-210℃的温度和190psig至275psig的压力和3至10分钟的曝光时间下进行。

8. 发明授权

US09255189B2 Ethanol production with two stage continuous steam pre-treatment of lignocellulosic biomass 有权
标题翻译：乙醇生产与两阶段连续蒸汽预处理木质纤维素生物质
公开(公告)号：US09255189B2
公开(公告)日：2016-02-09
申请号：US13554601
申请日：2012-07-20
申请人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
发明人： Frank A. Dottori , Robert Ashley Cooper Benson , Regis-Olivier Benech
IPC分类号： C12S3/02 , C08B37/00 , C08H8/00
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16 , Y02E50/343
摘要： Disclosed is a method of producing ethanol from lignocellulosic biomass, including a two stage steam pretreatment process. The first stage of the steam pretreatment is carried out by heating the biomass with high pressure steam to a first stage temperature of 140° C. to 180° C. for a first stage time of 30 minutes to 2 hours at a first stage pressure of 105 to 150 psig; and the second steam pretreatment stage is carried out by heating the biomass with high pressure steam to raise the biomass temperature to a second stage temperature of 190° C. to 210° C. for a second stage time of 2 to 10 minutes at a second stage pressure of 167 to 262 psig. Hemicellulose and inhibitors (inhibitory compounds) to downstream hydrolysis and fermentation are preferably removed between the first and second pretreating stages, more preferably after each pretreatment stage.
摘要翻译：公开了一种从木质纤维素生物质生产乙醇的方法，包括两阶段蒸汽预处理方法。蒸汽预处理的第一阶段是通过用高压蒸汽将生物质加热至140℃至180℃的第一阶段温度进行，第一阶段时间为30分钟至2小时，在第一阶段的压力 105至150 psig; 并且通过用高压蒸汽加热生物质来进行第二蒸汽预处理阶段，以将生物质温度升至190℃至210℃的第二阶段温度，第二阶段时间为2至10分钟阶段压力为167至262psig。优选在第一和第二预处理阶段之间去除下游水解和发酵的半纤维素和抑制剂（抑制化合物），更优选在每个预处理阶段之后。

9. 发明授权

US08642303B2 Process for alcoholic fermentation of lignocellulosic biomass 有权
标题翻译：木质纤维素生物质酒精发酵工艺
公开(公告)号：US08642303B2
公开(公告)日：2014-02-04
申请号：US12644935
申请日：2009-12-22
申请人： Robert Ashley Cooper Benson , Regis-Olivier Benech
发明人： Robert Ashley Cooper Benson , Regis-Olivier Benech
IPC分类号： C07K1/00 , C12P1/00 , C12P7/00 , C12P7/02 , C12P7/06 , C12P7/10 , D21C1/00
CPC分类号： C12P7/10 , Y02E50/16 , Y02E50/17
摘要： A process for the production of ethanol wherein a hydrolyzed lignocellulosic biomass is fermented in the presence of a stillage residue. The fermentation of cellulosic hydrolysates is improved by adding prior to and/or during fermentation a stillage residue side stream from a corn starch-to-ethanol process as a nutrient source for the yeast organisms used in the fermentation. Stillage residues from the grain dry mill ethanol producing process, including the whole stillage, wet cake, thin stillage, and/or syrup are added to assist as a nitrogen and nutrient source for the fermentive processes. The stillage residue is produced by any grain-to-ethanol process.
摘要翻译：一种生产乙醇的方法，其中水解的木质纤维素生物质在釜馏残渣存在下发酵。纤维素水解产物的发酵通过在发酵之前和/或发酵过程中添加作为用于发酵的酵母生物的营养源的玉米淀粉 - 乙醇方法的釜渣残留物流进行改进。添加来自谷物干磨乙醇生产过程的残留物，包括整个釜渣，湿饼，薄釜渣和/或糖浆，以辅助作为发酵过程的氮和营养源。釜渣残留物通过任何谷物 - 乙醇方法生产。

10. 发明申请

US20130017589A1 FRACTIONATION OF LIGNOCELLULOSIC BIOMASS FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION 审中-公开
标题翻译：纤维素乙醇纤维素生物质的分解和化学生产
公开(公告)号：US20130017589A1
公开(公告)日：2013-01-17
申请号：US13551087
申请日：2012-07-17
申请人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
发明人： Frank A. DOTTORI , Robert Ashley Cooper BENSON , Regis-Olivier BENECH
IPC分类号： D21B1/36 , C12P7/10
CPC分类号： D21B1/36 , C12P7/10 , C13K1/02 , C13K13/002 , Y02E50/16
摘要： A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.
摘要翻译：定义了一种用于玉米芯和低木质素木质纤维素生物质的连续蒸汽预处理和分馏以产生对酶水解敏感的浓缩纤维素固体物流的方法。通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。生产用于发酵的生物燃料的纤维素衍生的葡萄糖。回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

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