专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20140053300A1 METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE 审中-公开
标题翻译：单体植物细胞系生成方法
公开(公告)号：US20140053300A1
公开(公告)日：2014-02-20
申请号：US14115629
申请日：2011-06-07
申请人： Janina Kirchhoff , Stefan Schillberg , Andreas Schiermeyer , Helga Schinkel , Rainer Fischer
发明人： Janina Kirchhoff , Stefan Schillberg , Andreas Schiermeyer , Helga Schinkel , Rainer Fischer
IPC分类号： C12N15/82
摘要： The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.
摘要翻译：本发明提供了从异源植物细胞群体产生单克隆植物细胞系的方法，包括以下步骤：（a）提供异源植物细胞群; （b）从所述异源植物细胞群体制备原生质体; （c）通过使原生质体的制备物流式细胞分选来分离单个原生质体; （d）分离的单一转化的原生质体的再生直到在饲养细胞材料存在下共培养形成微菌落; （e）从饲养细胞材料中除去微菌落并培养微菌落直到形成单克隆植物细胞系。

2. 发明申请

US20130108662A1 PEPTIDES USEFUL AS CELL-PENETRATING PEPTIDES 审中-公开
标题翻译：肽可用作细胞穿刺肽
公开(公告)号：US20130108662A1
公开(公告)日：2013-05-02
申请号：US13557706
申请日：2012-07-25
申请人： Roland Brock , Rainer Fischer , Mariola Fotin-Mleczek , Hansjoerg Hufnagel , Norbert Windhab
发明人： Roland Brock , Rainer Fischer , Mariola Fotin-Mleczek , Hansjoerg Hufnagel , Norbert Windhab
IPC分类号： C07K14/47
CPC分类号： C07K14/47 , A61K31/713 , A61K39/385 , A61K47/644 , A61K51/088 , A61K2039/53 , C07K14/79
摘要： A peptide having an amino acid sequence containing at least eight consecutive amino acids of the human lactoferrin protein or of the bovine lactoferrin protein. The peptide is suitable as a cell-penetrating peptide. A complex of the peptide and a cargo molecule non-covalently bound to the peptide. The cargo molecule may be a nucleic acid, an amino acid, a peptide, a protein, a carbohydrate, a lipid, or a small molecule. A method of penetrating or transfecting a cell using the complex.
摘要翻译：具有含有人乳铁蛋白蛋白质或牛乳铁蛋白蛋白质的至少8个连续氨基酸的氨基酸序列的肽。该肽适合作为细胞穿透肽。肽和与肽共价结合的货物分子的复合物。载体分子可以是核酸，氨基酸，肽，蛋白质，碳水化合物，脂质或小分子。使用复合物渗透或转染细胞的方法。

3. 发明申请

US20130000591A1 PIN BOSSES OF A PISTON FOR AN INTERNAL COMBUSTION ENGINE 有权
标题翻译：内燃机活塞密封件
公开(公告)号：US20130000591A1
公开(公告)日：2013-01-03
申请号：US13513303
申请日：2010-12-02
申请人： Harald Boekle , Gottfried Schnaitmann , Rainer Fischer , Ralf Braig , Helmut Mueller
发明人： Harald Boekle , Gottfried Schnaitmann , Rainer Fischer , Ralf Braig , Helmut Mueller
IPC分类号： F02F3/00
CPC分类号： F16J1/16 , B23P15/10 , F02F2200/06 , Y10T29/49249 , Y10T29/49266
摘要： The invention relates to pin bosses (1) of a piston (2) for an internal combustion engine, each comprising a pin bore (3) for supporting a piston pin (14). In order to reduce the tangential stresses in the zenith area of the pin bores and thus to extend the service life of the piston, the inner areas (16) of the pin bores (3) as viewed in the direction of the bore axis (13) have areas on both sides of the zenith (4) that have a greater distance from the bore axis (13) than the zenith (4) of the pin bore (3), and the outer areas (17) of the pin bores (3) as viewed in the direction of the bore axis (13) are transversely oval relative to the piston axis (7).
摘要翻译：本发明涉及一种用于内燃机的活塞（2）的销凸台（1），每个都包括用于支撑活塞销（14）的销孔（3）。为了减小销孔的顶点区域中的切向应力，从而延长活塞的使用寿命，从孔轴线（13）的方向观察时，销孔（3）的内部区域（16））在天顶（4）的两侧具有距钻孔轴线（13）比销孔（3）的天顶（4）更远的距离，以及销孔（2）的外部区域（17）如图3所示，从孔轴线13的方向观察，相对于活塞轴线7是横向椭圆形的。

4. 发明申请

US20120042416A1 Antibody fusion-mediated plant resistance against Oomycota 审中-公开
标题翻译：抗体融合介导的植物对卵菌的抗性
公开(公告)号：US20120042416A1
公开(公告)日：2012-02-16
申请号：US13264224
申请日：2010-08-05
申请人： Sylvia Schleker , Dieter Peschen , Rainer Fischer , Stefan Schillberg
发明人： Sylvia Schleker , Dieter Peschen , Rainer Fischer , Stefan Schillberg
IPC分类号： A01H5/00 , C12N15/62 , C12P21/02 , C12N15/82 , A01H1/06 , C12N5/10 , A01N37/18 , A01P3/00 , A01N43/90 , C40B40/10 , G01N33/566 , C12Q1/04 , C12N1/21 , C12N1/19 , C12P21/00 , A01N43/50 , C12Q1/68 , C07K19/00
CPC分类号： C12N15/8282 , A01N63/00 , A01N63/02 , C07K14/43563 , C07K14/43581 , C07K14/46 , C07K16/14 , C07K2317/622 , C07K2319/00 , C07K2319/55 , C12N15/8258
摘要： The present invention relates to fusion proteins comprising anti-Oomycotic proteins or peptides linked to an antibody or fragment thereof specifically recognising an epitope of an Oomycota. The invention is also directed to polynucleotides coding for the fusion proteins. The embodiments of the present invention are particularly useful for the protection of plants against Oomycota. The invention therefore comprises transgenic plants expressing the fusion proteins of the present invention.
摘要翻译：本发明涉及包含抗卵母细胞蛋白或与特异性识别卵菌纲表位的抗体或其片段连接的肽的融合蛋白。本发明还涉及编码融合蛋白的多核苷酸。本发明的实施方案特别适用于保护植物免受卵菌感染。因此，本发明包括表达本发明的融合蛋白的转基因植物。

5. 发明申请

US20100035241A1 Isolation Of Allergen-Specific Immunoglobulin Genes From Human B-Cells From Atopy Sufferers 有权
标题翻译：从特应性受试者的人类B细胞中分离过敏原特异性免疫球蛋白基因
公开(公告)号：US20100035241A1
公开(公告)日：2010-02-11
申请号：US11597492
申请日：2005-05-25
申请人： Gernot Achatz , Michael Huhn , Rainer Fischer , Fatima Ferreira , Elke Luger , Michael Stöcker , Stefan Barth , Torsten Klockenbring
发明人： Gernot Achatz , Michael Huhn , Rainer Fischer , Fatima Ferreira , Elke Luger , Michael Stöcker , Stefan Barth , Torsten Klockenbring
IPC分类号： C12Q1/68 , C07H21/00 , C07K16/00 , G01N33/53 , C12N15/63
CPC分类号： C12N15/1034 , C07K16/16 , C07K2317/622
摘要： A process is disclosed which enables the establishing of comprehensive immunoglobulin-specific reaction profiles of subjects with disorders of the immune function by the individual V gene repertoire of Ig-expressing B cells. The process comprises the isolation of B cells from body fluids, the isolation of individual B cells and their genetic material, the amplification of nucleic acids coding the variable regions of an antibody expressed by a B cell, the recombinant preparation of antibodies by expressing the amplificates, and the determination of the binding
摘要翻译：公开了一种方法，其能够通过单克隆抗体表达B细胞的V基因库，建立具有免疫功能障碍的受试者的综合免疫球蛋白特异性反应谱。该方法包括从体液中分离B细胞，分离个体B细胞及其遗传物质，编码由B细胞表达的抗体的可变区的核酸的扩增，通过表达扩增产物的重组抗体制备，并确定绑定

6. 发明申请

US20050059053A1 Complex formation for the stabilisation and purification of proteins of interest 审中-公开
标题翻译：用于稳定和纯化目的蛋白质的复合物形成
公开(公告)号：US20050059053A1
公开(公告)日：2005-03-17
申请号：US10895399
申请日：2004-07-21
申请人： Rainer Fischer , Markus Sack , Carmen Vaquero-Martin
发明人： Rainer Fischer , Markus Sack , Carmen Vaquero-Martin
IPC分类号： C07H21/04 , C07K14/705 , C07K16/30 , C12N15/62 , C12N15/82 , C12P21/02 , C12Q1/68 , C12P21/04 , G01N33/53
CPC分类号： C12N15/62 , C07H21/04 , C07K14/70503 , C07K16/3007 , C07K2317/13 , C07K2317/622 , C07K2317/626 , C07K2319/033 , C07K2319/21 , C07K2319/40 , C12N15/8257 , C12N15/8258 , C12P21/02
摘要： A method is described for altering the properties such as the accumulation, the stability and/or integrity, the subcellular localisation, the post-translational modifications, the ability to get purified, and the phase partitioning behaviour of natural or recombinant target proteins expressed in a host organism. The method involves the co-expression of natural or recombinant proteins along with a specific binding partner that sequesters the target recombinant protein into a complex. The binding partner is supplied as a separate protein allowing formation of intermolecular complexes or is fused to the protein of interest, allowing the formation of intramolecular complexes. The binding partner can also be used to alter the subcellular localisation without modifying the sequence or structure of the target protein itself. This can be achieved by either incorporating appropriate targeting signals into the binding ligand, which are then linked to the target protein through complex formation, or complex formation itself may alter the subcellular localisation. The same strategy can be used to provide an affinity tag to facilitate protein purification. The principle of the invention is demonstrated by the coexpression of an unstable antibody and its cognate antigen.
摘要翻译：描述了一种用于改变诸如累积，稳定性和/或完整性，亚细胞定位，翻译后修饰，获得纯化的能力以及表达于天然或重组靶蛋白的相分离行为的性质的方法宿主生物。该方法包括将天然或重组蛋白与特异性结合配偶体共表达，所述特异性结合配偶体将靶重组蛋白质螯合到复合物中。结合配偶体作为单独的蛋白质提供，允许形成分子间复合物或者与感兴趣的蛋白质融合，从而形成分子内复合物。结合配偶体也可用于改变亚细胞定位，而不改变靶蛋白本身的序列或结构。这可以通过将适当的靶向信号并入结合配体中来实现，然后通过复合物形成与靶蛋白连接，或者复合物形成本身可能改变亚细胞定位。可以使用相同的策略来提供亲和标签以促进蛋白质纯化。本发明的原理通过不稳定抗体及其同源抗原的共表达来证明。

7. 发明授权

US3164646A Method and apparatus for the production of bottles and similar hollow bodies from thermoplastic synthetic substances 失效
标题翻译：用于从热塑性合成物质制造瓶子和类似中空体的方法和装置
公开(公告)号：US3164646A
公开(公告)日：1965-01-05
申请号：US15379861
申请日：1961-11-21
申请人： RAINER FISCHER
发明人： RAINER FISCHER
IPC分类号： B29C49/76
CPC分类号： B29C49/76

8. 发明授权

US10480001B2 Method for the generation of a monoclonal plant cell line 有权
公开(公告)号：US10480001B2
公开(公告)日：2019-11-19
申请号：US14115629
申请日：2011-06-07
申请人： Janina Kirchhoff , Stefan Schillberg , Andreas Schiermeyer , Helga Schinkel , Rainer Fischer
发明人： Janina Kirchhoff , Stefan Schillberg , Andreas Schiermeyer , Helga Schinkel , Rainer Fischer
IPC分类号： C12N15/82 , A01H4/00
摘要： The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.

9. 发明授权

US08752521B2 Piston 有权
标题翻译：活塞
公开(公告)号：US08752521B2
公开(公告)日：2014-06-17
申请号：US13221498
申请日：2011-08-30
申请人： Ralf Braig , Rainer Fischer , Thomas Hettich , Peter Kleinle , Michael Marquardt
发明人： Ralf Braig , Rainer Fischer , Thomas Hettich , Peter Kleinle , Michael Marquardt
IPC分类号： F02F3/00
CPC分类号： F02F3/02
摘要： A piston for an internal combustion engine may include a piston head and a piston shaft. The piston shaft may have two shaft walls, one arranged on a pressure side and the other arranged on a counter-pressure side. Two box walls may have pin bosses and connect the shaft walls. Each of the box walls may have a first partial wall section and a second partial wall section. The first partial wall section may extend between the pin boss and the shaft wall arranged on the pressure side. The second partial wall section may extend between the pin boss and the shaft wall arranged on the counter-pressure side. A first longitudinal center line of the first partial wall section is may be convergent to a second longitudinal center line of the second partial wall section, wherein the first and second longitudinal center lines—intersect each other outside the piston.
摘要翻译：用于内燃机的活塞可以包括活塞头和活塞轴。活塞轴可以具有两个轴壁，一个布置在压力侧，另一个布置在反压侧。两个箱壁可以具有销凸起并连接轴壁。每个箱壁可以具有第一部分壁部分和第二部分壁部分。第一部分壁部分可以在销凸台和布置在压力侧的轴壁之间延伸。第二部分壁部分可以在销凸台和布置在反压侧的轴壁之间延伸。第一部分壁部分的第一纵向中心线可以会聚到第二部分壁部分的第二纵向中心线，其中第一和第二纵向中心线在活塞外部彼此相交。

10. 发明授权

US08605210B2 Optical module for a cellular phone 有权
标题翻译：用于蜂窝电话的光模块
公开(公告)号：US08605210B2
公开(公告)日：2013-12-10
申请号：US12996909
申请日：2009-09-10
申请人： Sebastien Fabre , Rainer Fischer , Mathias Schulz
发明人： Sebastien Fabre , Rainer Fischer , Mathias Schulz
IPC分类号： H04N5/225
CPC分类号： H04N5/335 , H04N5/2253 , H04N5/2257 , H05K1/182
摘要： The invention relates to an optical module for a cellular phone having an image sensor comprising a light sensitive upper side for detecting and converting optical signals into electrical signals, an imaging device for imaging the optical signals on the image sensor, and a circuit board comprising an upper side and a lower side electrically and mechanically connected to the image sensor. In order to create a particularly flat optical module, for example for integration into a cellular phone, the invention provides that the circuit board has a recess in which the image sensor is disposed such that a lower side of the image sensor is substantially located at the same height as the lower side of the circuit board.
摘要翻译：本发明涉及一种用于蜂窝电话的光学模块，其具有图像传感器，该图像传感器包括用于检测和将光信号转换为电信号的光敏上侧，用于对图像传感器上的光信号进行成像的成像装置，以及包括上侧和下侧电气地和机械地连接到图像传感器。为了创建特别平坦的光学模块，例如用于集成到蜂窝电话中，本发明提供了电路板具有凹部，图像传感器设置在该凹部中，使得图像传感器的下侧基本上位于与电路板下侧相同的高度。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式