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1. 发明申请

US20140024040A1 METHODS AND KITS FOR BREAKING EMULSIONS 有权
标题翻译：破乳乳液的方法和工具
公开(公告)号：US20140024040A1
公开(公告)日：2014-01-23
申请号：US14110695
申请日：2012-04-05
申请人： Jeffrey Sabina , Ilya Zlatkovsky , Rachel Kasinskas
发明人： Jeffrey Sabina , Ilya Zlatkovsky , Rachel Kasinskas
IPC分类号： C07H21/00 , C07K1/14
CPC分类号： C07H21/00 , C07K1/145 , C12Q1/6806 , C12Q1/6874 , G01N1/4055 , C12Q2523/32 , C12Q2563/161 , C12Q2535/122
摘要： The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture. In some embodiments, the disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.
摘要翻译：本公开一般涉及用于破坏油包水乳液的方法，系统，组合物和试剂盒，其包括分散在油包水乳液的水相中的一种或多种生物分子。在一些实施方案中，本公开涉及获得包含连续疏水部分和不连续含水部分的第一乳液，所述水性部分具有分散在其中的一种或多种生物分子，通过使第一乳液与包含第二乳液乳液，其中第二乳液包括分散在连续水相中的有机萃取溶剂的不连续相，并离心以分离所得混合物的相。在一些实施方案中，本公开一般涉及用于从油包水乳液中提取生物分子的方法，试剂盒和系统，包括使用破碎溶液破坏包含连续疏水部分中的多个水滴的油包水乳液以产生所得到的含有一种或多种生物分子的反应混合物并操纵所得反应混合物以形成至少两相，其中一相包括含有一种或多种生物分子的水相。

2. 发明授权

US09617296B2 Methods and kits for breaking emulsions 有权
公开(公告)号：US09617296B2
公开(公告)日：2017-04-11
申请号：US14110695
申请日：2012-04-05
申请人： Jeffrey Sabina , Ilya Zlatkovsky , Rachel Kasinskas
发明人： Jeffrey Sabina , Ilya Zlatkovsky , Rachel Kasinskas
IPC分类号： C07H21/00 , C07K1/14 , C12Q1/68
CPC分类号： C07H21/00 , C07K1/145 , C12Q1/6806 , C12Q1/6874 , G01N1/4055 , C12Q2523/32 , C12Q2563/161 , C12Q2535/122
摘要： The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture. In some embodiments, the disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.

3. 发明申请

US20120156728A1 CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING 审中-公开
标题翻译：核酸在固体表面的克隆放大与模板运动
公开(公告)号：US20120156728A1
公开(公告)日：2012-06-21
申请号：US13328844
申请日：2011-12-16
申请人： Bin Li , Kai Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
发明人： Bin Li , Kai Lao , Jennifer O'Neil , Jennifer Kunkel , Kellie Haley , Rachel Kasinskas , Zhaochun Ma , Pius Brzoska
IPC分类号： C12P19/34
摘要： Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
摘要翻译：提供了在载体上产生固定化克隆扩增子的局部群体的新方法。

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