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1. 发明申请

US20090124008A1 PEPTIDES FOR USE IN CULTURE MEDIA 审中-公开
标题翻译：用于文化媒体的肽
公开(公告)号：US20090124008A1
公开(公告)日：2009-05-14
申请号：US11835975
申请日：2007-08-08
申请人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Bruce Wayne Erickson , Ann Hart Erickson
发明人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Bruce Wayne Erickson , Ann Hart Erickson
IPC分类号： C12N5/06 , C12N5/02
CPC分类号： C07K7/06 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C40B30/06
摘要： The present invention provides peptides libraries which are useful for rapid identification of biologically active compounds. The invention further provides peptides which include cell-growth affecting peptides and peptides which enhance or inhibit production of cellular proteins. Many of the peptides of the invention may be produced in large quantity by recombinant techniques and formulated in culture medium to produce the desired effect on cultured cells and tissues. Certain of the libraries of the invention and the peptides identified in them are particularly useful in concatemer-based recombinant expression methods.
摘要翻译：本发明提供了可用于快速鉴定生物活性化合物的肽文库。本发明还提供了包括增强或抑制细胞蛋白产生的细胞生长影响肽和肽的肽。本发明的许多肽可以通过重组技术大量生产并配制在培养基中以对培养的细胞和组织产生所需的效果。本发明的某些文库和其中鉴定的肽在基于连接体的重组表达方法中特别有用。

2. 发明授权

US5863736A Method, apparatus and computer program products for determining quantities of nucleic acid sequences in samples 失效
标题翻译：用于确定样品中核酸序列数量的方法，装置和计算机程序产品
公开(公告)号：US5863736A
公开(公告)日：1999-01-26
申请号：US862905
申请日：1997-05-23
申请人： Perry D. Haaland
发明人： Perry D. Haaland
IPC分类号： G01N33/50 , C12M1/00 , C12N15/09 , C12Q1/68 , C12P19/34 , G06F17/21
CPC分类号： C12Q1/6851
摘要： Methods for determining quantities of nucleic acid sequences in samples include the steps of amplifying a plurality of known quantities of a nucleic acid sequence in respective calibration samples and an unknown quantity of a nucleic acid sequence in a test sample, in parallel, during a time interval. These samples may be amplified using an isothermal amplification method such as Strand Displacement Amplification (SDA), or a thermal cycling amplification method such as Polymerase Chain Reaction (PCR), for example. Indicia of the quantities of a nucleic acid sequence being amplified in the calibration and test samples are then measured using conventional techniques, at measurement points in the time interval. Steps are then performed to determine for a first potential cutoff level, a corresponding first set of time points in the time interval at which the indicia of the quantities of a nucleic acid sequence being amplified in each of the calibration samples equal the first cutoff level. This step is then repeated for each of a number of different potential cutoff levels so that respective sets of time points in the time interval can be obtained for each potential cutoff level. According to a preferred aspect of the present invention, a step is then performed to determine, relative to a statistical criterion, which of the sets of points in the time interval better satisfies the statistical criterion against the known quantities of a nucleic acid sequence in the calibration samples. A quantity of a nucleic acid sequence in the test sample is then determined based on the set of points determined to better or best satisfy the statistical criterion. The accuracy of the determination can also be improved using a relatively large number of potential cutoff levels.
摘要翻译：用于确定样品中核酸序列数量的方法包括以下步骤：在时间间隔期间并行扩增多个已知量的各校准样品中的核酸序列和测试样品中未知量的核酸序列。这些样品可以使用例如Strand Displacement Amplification（SDA）等等温扩增方法或者例如聚合酶链式反应（PCR）等热循环扩增方法进行扩增。然后在时间间隔的测量点使用常规技术测量在校准和测试样品中扩增的核酸序列的量的标记。然后执行步骤以确定第一潜在截止水平，即在每个校准样本中放大的核酸序列的量的标记等于第一截止水平的时间间隔内相应的第一组时间点。然后对于多个不同的潜在截止电平中的每一个重复该步骤，使得可以针对每个潜在截止电平获得时间间隔中的各组时间点。根据本发明的优选方面，然后执行步骤以相对于统计标准确定时间间隔中的哪组点更好地满足统计标准中的核酸序列的已知量校准样品。然后基于确定为更好或最好地满足统计标准的一组点来确定测试样品中的一定数量的核酸序列。也可以使用相对大量的潜在截止水平来提高确定的准确度。

3. 发明授权

US06216049B1 Computerized method and apparatus for analyzing nucleic acid assay readings 有权
标题翻译：用于分析核酸测定读数的计算机化方法和装置
公开(公告)号：US06216049B1
公开(公告)日：2001-04-10
申请号：US09196123
申请日：1998-11-20
申请人： Harry Yang , Daniel L. Schwarz , Christopher M. Embres , Richard L. Moore , Perry D. Haaland , Paula V. Johnson
发明人： Harry Yang , Daniel L. Schwarz , Christopher M. Embres , Richard L. Moore , Perry D. Haaland , Paula V. Johnson
IPC分类号： G05B1302
CPC分类号： G06F19/20
摘要： A computerized method and apparatus are disclosed for analyzing numerical data pertaining to a sample assay comprising at least one biological or chemical sample. The data include a set of data pertaining to each respective sample, with each set of data including a plurality of values each representing a condition of the sample at a given time. The method and apparatus assign a respective numerical value to each of the data values, mathematically combine the numerical values to generate a total value, compare the total value to a threshold value, and control the system to indicate whether the sample has a predetermined characteristic based on a result of the comparison. Prior to calculation of the sample value, filtering, normalizing and other correcting operations can be performed on the data to correct anomalous values in the data which could adversely affect the accuracy of the results. The method and apparatus perform the described functions by representing the data values as points on a graph having a vertical axis representing the magnitudes of the values and a horizontal axis representing a period of time during which readings of the sample were taken to obtain the data values, identifying points on the graph having an anomalous characteristic, and correcting the anomalous points to produce a corrected plot of points on the graph, with each of the points of the corrected plot representing a magnitude of a corresponding one of the values. An area value is then calculated which represents an approximate area between at least a portion of the corrected plot of points on the graph and the horizontal axis. The area value is compared to a threshold value to determine whether a certain condition exists in the sample to which the set of data pertains.
摘要翻译：公开了一种用于分析与包含至少一种生物或化学样品的样品测定有关的数值数据的计算机化方法和装置。数据包括与每个相应样本相关的一组数据，每组数据包括多个值，每个值表示在给定时间的样本的状况。该方法和装置为每个数据值分配相应的数值，数学地组合数值以产生总值，将总值与阈值进行比较，并且控制系统以指示样本是否具有基于预定特征的比较的结果。在计算样本值之前，可以对数据执行滤波，归一化和其他校正操作，以纠正数据中可能对结果准确性产生不利影响的异常值。该方法和装置通过将数据值表示为具有表示值的大小的垂直轴的图形的数据值和表示采样的读数以获得数据值的时间段的水平轴来执行所述功能识别具有异常特征的图上的点，并且校正异常点以产生曲线图上的点的校正图，其中校正曲线的每个点表示对应的一个值的大小。然后计算面积值，其表示曲线图上的点的校正曲线的至少一部分与水平轴之间的近似面积。将面积值与阈值进行比较，以确定在该组数据所属的样本中是否存在特定条件。

4. 发明授权

US6066458A Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates 失效
标题翻译：用于使用标准曲线和扩增率估计来确定样品中核酸序列数量的方法，装置和计算机程序产品
公开(公告)号：US6066458A
公开(公告)日：2000-05-23
申请号：US80589
申请日：1998-05-18
申请人： Perry D. Haaland , James G. Nadeau , Colleen M. Nycz , Cheryl H. Dean , Catherine A. Spargo
发明人： Perry D. Haaland , James G. Nadeau , Colleen M. Nycz , Cheryl H. Dean , Catherine A. Spargo
IPC分类号： C12N15/00 , C12Q1/68
CPC分类号： C12Q1/6851
摘要： Methods for determining quantities of nucleic acid sequences in samples undergoing amplification utilize amplification ratio estimates (R*) in operations to accurately perform absolute quantitation even when amplification factors for the target and control sequences undergoing amplification are different, time dependent or vary as a function of starting concentrations of nucleic acid sequences. These operations also take into account conversion efficiencies associated with the conversion of probes upon generation of target or control amplicons, but do not require the explicit calculation of such efficiencies. The operations also recognize that a preferred R* should be determined based on a preferred statistical criterion to improve quantitation. In addition, the use of standard samples having known starting concentrations of target and control sequences therein may enable accurate absolute quantitation without the explicit calculation of amplification ratio estimates.
摘要翻译：用于确定正在进行扩增的样品中核酸序列数量的方法利用操作中的扩增比率估计（R *）来准确地执行绝对定量，即使目标和经历放大的控制序列的放大因子不同，时间依赖或随着核酸序列的起始浓度。这些操作还考虑了在靶或扩增子产生时与探针转化相关的转化效率，但不需要明确计算这种效率。操作还认识到，优选的R *应基于优选的统计标准来确定，以改进定量。此外，使用具有已知起始浓度的目标和控制序列的标准样品可以使得能够进行精确的绝对定量，而无需显式计算扩增率估计值。

5. 发明授权

US07267983B2 Peptides for use in culture media 失效
标题翻译：用于培养基的肽
公开(公告)号：US07267983B2
公开(公告)日：2007-09-11
申请号：US10841056
申请日：2004-05-07
申请人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Ann Hart Erickson, legal representative
发明人： Bruce Wayne Erickson, deceased
IPC分类号： C12N5/00 , C40B30/40
CPC分类号： C07K7/06 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C40B30/06
摘要： The present invention provides peptides libraries which are useful for rapid identification of biologically active compounds. The invention further provides peptides which include cell-growth affecting peptides and peptides which enhance or inhibit production of cellular proteins. Many of the peptides of the invention may be produced in large quantity by recombinant techniques and formulated in culture medium to produce the desired effect on cultured cells and tissues. Certain of the libraries of the invention and the peptides identified in them are particularly useful in concatemer-based recombinant expression methods.
摘要翻译：本发明提供了可用于快速鉴定生物活性化合物的肽文库。本发明还提供了包括增强或抑制细胞蛋白产生的细胞生长影响肽和肽的肽。本发明的许多肽可以通过重组技术大量生产并配制在培养基中以对培养的细胞和组织产生所需的效果。本发明的某些文库和其中鉴定的肽在基于连接体的重组表达方法中特别有用。

6. 发明授权

US07742877B1 Methods, apparatus and computer program products for formulating culture media 有权
标题翻译：用于制定培养基的方法，仪器和计算机程序产品
公开(公告)号：US07742877B1
公开(公告)日：2010-06-22
申请号：US09359260
申请日：1999-07-22
申请人： Robert L. Campbell , Perry D. Haaland , Douglas B. Sherman , Walter William Stewart, II , Sheila A. Lloyd
发明人： Robert L. Campbell , Perry D. Haaland , Douglas B. Sherman , Walter William Stewart, II , Sheila A. Lloyd
IPC分类号： G01N33/48 , G01N31/00 , G06G7/48 , G06G7/58
CPC分类号： G01N33/502 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/00734 , C12Q1/025 , C12Q1/045 , C40B40/06 , C40B40/10 , C40B40/12 , G01N33/5008
摘要： Methods, apparatus and computer program products are provided for identifying a component of culture medium based on the parameters (e.g., physical, chemical, biological and/or topological characteristics) of compounds from within a compound library. In preferred embodiments, the compound library is a peptide library. Also provided are methods of selecting a compound library from a larger compound space based on whole molecule (i.e., global) parameters of the compounds. Preferably, this method is practiced in conjunction with a method of identifying a component of a culture medium. Further provided are methods of predicting a biological activity of a peptide based on at least one whole molecule parameter of the peptide. This method finds use in methods of drug discovery, identifying components of culture medium, and identifying and/or designing peptides with particular pharmacological or therapeutic activities.
摘要翻译：基于来自化合物库内的化合物的参数（例如物理，化学，生物学和/或拓扑学特征），提供了用于鉴定培养基组分的方法，装置和计算机程序产品。在优选的实施方案中，化合物文库是肽文库。还提供了基于化合物的全分子（即全局）参数从较大化合物空间中选择化合物文库的方法。优选地，该方法结合识别培养基组分的方法来实施。还提供了基于肽的至少一个全分子参数预测肽的生物活性的方法。该方法用于药物发现的方法，鉴定培养基的组分，以及鉴定和/或设计具有特定药理学或治疗活性的肽。

7. 发明授权

US07344884B2 Peptides promoting cell adherence, growth and secretion 有权
标题翻译：促进细胞粘附，生长和分泌的肽
公开(公告)号：US07344884B2
公开(公告)日：2008-03-18
申请号：US11351132
申请日：2006-02-09
申请人： Robert L. Campbell , Mohammad Heidaran , Catherine A. Spargo , Jamie H. Wilkins , Perry D. Haaland
发明人： Robert L. Campbell , Mohammad Heidaran , Catherine A. Spargo , Jamie H. Wilkins , Perry D. Haaland
IPC分类号： C07K7/06 , C12N5/00
CPC分类号： C07K7/06 , A61K38/00 , C07K1/047 , C07K5/06086 , C07K5/0815 , C07K5/0817 , C07K5/1019
摘要： The present invention provides specific peptides identified as having cell adhesion, growth, expression or secretion-enhancing activities. Many of the peptides of the invention may be produced in large quantity by such means as chemical synthesis or recombinant DNA methodology. They may be non-specifically adsorbed, or chemically attached to a surface or, alternatively, formulated in a culture medium to produce the desired effect on cultured cells.
摘要翻译：本发明提供了鉴定为具有细胞粘附，生长，表达或分泌增强活性的特异性肽。本发明的许多肽可以通过化学合成或重组DNA方法等手段大量生产。它们可以非特异性吸附或化学连接到表面，或者配制在培养基中以对培养的细胞产生所需的效果。

8. 发明授权

US07179635B2 Peptides for use in culture media 有权
公开(公告)号：US07179635B2
公开(公告)日：2007-02-20
申请号：US10841121
申请日：2004-05-07
申请人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Ann Hart Erickson, legal representative
发明人： Bruce Wayne Erickson, deceased
IPC分类号： C12N1/20
CPC分类号： C07K7/06 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C40B30/06
摘要： The present invention provides peptides libraries which are useful for rapid identification of biologically active compounds. The invention further provides peptides which include cell-growth affecting peptides and peptides which enhance or inhibit production of cellular proteins. Many of the peptides of the invention may be produced in large quantity by recombinant techniques and formulated in culture medium to produce the desired effect on cultured cells and tissues. Certain of the libraries of the invention and the peptides identified in them are particularly useful in concatemer-based recombinant expression methods.

9. 发明授权

US06759510B1 Peptides for use in culture media 有权
公开(公告)号：US06759510B1
公开(公告)日：2004-07-06
申请号：US09608892
申请日：2000-06-30
申请人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Bruce Wayne Erickson
发明人： Perry D. Haaland , Douglas B. Sherman , Robert L. Campbell , Walter William Stewart , Sheila A. Lloyd , Bruce Wayne Erickson
IPC分类号： C07K700
CPC分类号： C07K7/06 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C40B30/06
摘要： The present invention provides peptides libraries which are useful for rapid identification of biologically active compounds. The invention further provides peptides which include cell-growth affecting peptides and peptides which enhance or inhibit production of cellular proteins. Many of the peptides of the invention may be produced in large quantity by recombinant techniques and formulated in culture medium to produce the desired effect on cultured cells and tissues. Certain of the libraries of the invention and the peptides identified in them are particularly useful in concatemer-based recombinant expression methods.

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