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    • 8. 发明申请
    • Method
    • 方法
    • US20050142647A1
    • 2005-06-30
    • US10898775
    • 2004-07-26
    • Paul WassellJorn SoeJorn MikkelsenAnna Kristensen
    • Paul WassellJorn SoeJorn MikkelsenAnna Kristensen
    • C12P7/64
    • C11C1/08C11C3/06C12N9/1029C12P7/6436C12P7/6445C12Y203/01
    • The present invention relates to a method of reducing and/or removing diglyceride from an edible oil, comprising a) admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. Preferably, the diglyceride:glycerol acyltransferase comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S. Furthermore the present invention relates to the use of a diglyceride:glycerol acyltransferase characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol, in the manufacture of an edible oil, for reducing and/or removing (preferably selectively reducing and/or removing) diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.
    • 本发明涉及一种从食用油中还原和/或除去甘油二酯的方法,其包括:a)将食用油与酰基受体底物和甘油二酯:甘油酰基转移酶混合,其中所述甘油二酯:甘油酰基转移酶的特征在于: 在食用油中能够将酰基从甘油二酯转移到甘油。 优选地,甘油二酯:甘油酰基转移酶包含氨基酸序列基序GDSX,其中X是以下氨基酸残基L,A,V,I,F,Y,H,Q,T,N,M或S中的一个或多个 此外,本发明涉及在制造食用油中特征在于在食用油中能够将酰基从甘油二酯转化为甘油的酶的甘油二酯:甘油酰基转移酶的用途,用于还原和/或 从所述食用油中除去(优选选择性还原和/或除去)甘油二酯,以及所述酶在制备包含用于改善所述食品的结晶性能的食用油的食品中的用途。